Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,605,953	G→A	Q301Q (CAG→CAA)	lsrB →	Autoinducer‑2 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,605,953	0	G	A	100.0%	53.4 / NA	18	Q301Q (CAG→CAA)	lsrB	Autoinducer‑2 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCTGGATT  >  NC_000913/1605711‑1606199                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCGGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGt                                                                                                                                                                                                                                                >  1:82607/1‑251 (MQ=255)                    cggccgccCAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAg                                                                                                                                                                                                                             <  1:142548/249‑1 (MQ=255)                             aaGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGAc                                                                                                                                                                                                                    <  1:449086/251‑1 (MQ=255)                                                       aaaGTAGCGATTGCAGGACTAAGTACGCCACATGCGATGCGCCCGTCTGTCGAAAGCGGCACGGTGAAAGCATTTGGCCTGTGGGCTGTGGTTCAGCCAGGCAAACTTTCAGTGTATGTCGCGGCTGCATTATTGAAAAAACGATCAATGAAAACGGGCGACAAGCTGGTTATCAAGGGCGTAGGCCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGACGCGGATGGTAATGGCATCgc                                                                                                                                                                                          >  2:195792/1‑250 (MQ=255)                                                                   gTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGa                                                                                                                                                                              <  1:305436/251‑1 (MQ=255)                                                                           cattACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGTCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATAGCAAGGGCGTAGTTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGTGCTATGACTACGAATCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTg                                                                                                                                                                       <  1:189375/247‑1 (MQ=255)                                                                                               gcCCGTATGTAGAACGCGGAACGGTGAAAGAATTTGGACTGCGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGAGGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCACCCAGCGTTCAGGGCTATGACTACGAAGCGGATGGGAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAACGAGAataac                                                                                                                                                  >  2:380073/1‑249 (MQ=255)                                                                                                   gTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGc                                                                                                                                               >  2:215691/1‑250 (MQ=255)                                                                                                       gTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTACAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAGCGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGGCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACGGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATa                                                                                                                                          <  1:210474/251‑1 (MQ=255)                                                                                                                                                  ttCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCTCGTGATATTCAACAAAGAGAATATCTGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCa                                                                                                <  2:264175/250‑1 (MQ=255)                                                                                                                                                  ttCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGc                                                                                                 >  1:342982/1‑249 (MQ=255)                                                                                                                                                             aaaaTTGCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGAAAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTAGCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCGAATACGATTTCGGATGTGCATTGGTTAACCTTAGTAAGTTATGGAAGATTTAGACGat                                                                                    <  2:185961/251‑1 (MQ=255)                                                                                                                                                                         tATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGt                                                                        >  1:242010/1‑251 (MQ=255)                                                                                                                                                                            gTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCGGATGTGCAGTACTGAACCGGAGTAAGTTAGGGCAGATTTATACGATATTAAAGATGGTaa                                                                      <  2:270247/250‑1 (MQ=255)                                                                                                                                                                               gcgGATGCATTATTGAACAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTCGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCACATACGATTTCTGATGTGCCTTACTTAACCGGAGTAAGTTATGGCGGATTTAGACGATATTAAAGATggaaa                                                                      >  2:52247/1‑244 (MQ=255)                                                                                                                                                                                                                     cGACAAGCTGGATATCACGGGCGTAGGTCAAGTTGAAGTCTCGCCAACCAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCACAAAAATATCCCTTTTAc                            >  1:175864/1‑251 (MQ=255)                                                                                                                                                                                                                              ggATATCAAGGGCGTAGGTCAAGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAgg                   <  1:215691/251‑1 (MQ=255)                                                                                                                                                                                                                                               gTCAAGTTGAAGTCTCGCCAAAAAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCTGGAtt  >  1:75031/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCGACAAGCTGGATATCAAGGGCGTAGGTCAGGTTGAAGTCTCGCCAAACAGCGTTCAGGGCTATGACTACGAAGCGGATGGTAATGGCATCGTACTGTTACCGGAGCGCGTGATATTCAACAAAGAGAATATCGGCAAATACGATTTCTGATGTGCATTACTTAACCGGAGTAAGTTATGGCAGATTTAGACGATATTAAAGATGGTAAAGATTTTCGTACCGATCAACCGCAAAAAAATATCCCTTTTACCCTGAAAGGTTGCGGTGCGCTGGATT  >  NC_000913/1605711‑1606199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑