Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,252,952	IS2 (–) +5 bp	coding (42‑46/357 nt)	yhaI →	putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	28 (0.690)	27/496	0.4	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	3252952 =	0 (0.000)					coding (42/357 nt)	yhaI	putative inner membrane protein
*	?	NC_000913	= 3187426	NA (NA)	24 (0.590)	24/496	0.5	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 3252956	0 (0.000)					coding (46/357 nt)	yhaI	putative inner membrane protein

CCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187188‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATTTTTTATAGGTAATTTATATAACGACATTGAAATATTTTAAATAAACATTAGTAAATTATTCATTCATCAAC  <  NC_000913/3252956‑3252718                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                CCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCA                                                                                                                                                                                                                                      <  1:279835/250‑1                  AGCAGAGAGCTTCGTGAAAACGTTAAATCGTTACTACATCATTATCATGCCCAAATCAGACGGGTGAGCGGCAGCTAAGAACCGTGTAGAGGCTTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGTGTTATCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCCCAGAGACACCAACATAATTTTTTAATAC                                                                                                                                                                                                                     <  2:143493/250‑1                        GAGCTTAGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGTCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCACCCCGGGAATATCTGCGGCAGCGAGCTTGCACTTGGTTCAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACAAACATAATTTTTTAATcaggtcaa                                                                                                                                                                                                               >  2:365029/1‑242                          GCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAACCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACTGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATA                                                                                                                                                                                                            <  2:340103/251‑1                                        ATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCGTAAAAA                                                                                                                                                                                              <  1:118939/251‑1                                                          AGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCATTAATTAAA                                                                                                                                                                              >  1:396246/1‑249                                                                       AACCAGACGGGTTAACGGCAGCAAATAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCT                                                                                                                                                                >  1:363986/1‑250                                                                                              AAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGACATCCGCATCGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGAGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTATATTACGGGTATTGTGTAAATGCT                                                                                                                                         >  1:133500/1‑250                                                                                               AGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTAT                                                                                                                                       <  1:203895/251‑1                                                                                                         AGAGGCGTTCGAGCATTATAACGAATGGCATCAGCATAGTGCGCTGGGTTATCGCTCGACACGGGAATATCTGCGGCAGCGGGCTTGAAATGGGTTAAGTGATACCAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGAACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTT                                                                                                                             >  2:42353/1‑251                                                                                                                TTCGAGCATTATAACGAATGGCATCCGCATAGTTCGCTTGGTTATCGCTCGCCACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGATAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAATAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCTTATCTTTCTTTTT                                                                                                                       <  2:175677/250‑1                                                                                                                 ttcAGCATTATAACGGATGGCATCCGCATATTGCGCTTGGTTATCGCTTGCCACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATATTTTTGTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGTTGAAAGTCTATTCCGGGTATTGTTGAAATGCTATTCGTAGCTTTCTTTTTC                                                                                                                      <  2:279406/247‑1                                                                                                                                      ATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATA                                                                                                <  1:33436/251‑1                                                                                                                                          GCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTA                                                                                            <  2:426850/251‑1                                                                                                                                           CATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCGTGTAATAGGATACGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGAATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTA                                                                                            >  2:345341/1‑250                                                                                                                                              AGTGCGCTGGGTTATCGCCCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGTTCAAATCCACAGATAAACCAACATAATTTTGTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTAGTTCGGGTATTGTGTAAATGCTTTTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGAT                                                                                        <  1:49632/251‑1                                                                                                                                                TGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATA                                                                                       >  1:211052/1‑250                                                                                                                                                             CGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGCTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATT                                                                         >  1:238614/1‑251                                                                                                                                                                    CACGGGAATATCTGCAGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGTACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGTTTATTTTTTATA                                                                  <  2:78539/251‑1                                                                                                                                                                                         GGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATTT                                                                        <  1:364461/224‑1                                                                                                                                                                                         GGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATTT                                                                        >  2:364461/1‑224                                                                                                                                                                                                           GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATT                                                                                                                                                                                 <  1:29688/101‑1                                                                                                                                                                                                           GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATT                                                                                                                                                                                 >  2:29688/1‑101                                                                                                                                                                                                                                     GGGCAAATCCACAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATTTTTTATAGGTAATTTATATAACGACATTGAAATATTTTAAATAAACATTAGTAAATTATTCATTCATCAAC  >  2:363093/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                CCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187188‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGAGAAACCAACATAATTTTTTAATACGGACAAATACCACTGCATAAAAACATCCCTTTAATTAAAAGTGACGAAAGTCTATTCCGGGTATTGTGTAAATGCTATTCGTATCTTTCTTTTTCGGATGTTATAAAGGTTTTGATATTTAAGATAAATACATGGTTATTTTTTATAGGTAATTTATATAACGACATTGAAATATTTTAAATAAACATTAGTAAATTATTCATTCATCAAC  <  NC_000913/3252956‑3252718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.