| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,869,314 | G→A | S120S (TCG→TCA) | yeaH → | DUF444 domain‑containing protein YeaH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,869,314 | 0 | G | A | 96.9% | 99.6 / ‑3.7 | 32 | S120S (TCG→TCA) | yeaH | DUF444 domain‑containing protein YeaH |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (14/17); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01 | |||||||||||
GTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACA > NC_000913/1869076‑1869555 | gTTCGGTGACGGACGTCGTCAGCGGCGAATCCGTATCCATTCCCACTGAAGATATTAGCGAACCTATGTTTCATCAGGGGCGTGGCGGTCTGCGCCTCCGCGTGCATCCGGGCAATGACCATGTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTTGCGGCGGAGGTTCCGGCAGTGGTCAGTGCCAGGCCAGCCAGGATGGTGAAGGTCATGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTAt < 1:429706/251‑1 (MQ=255) tCGGTGACGGACGTGGACAGCGGCGAATCCGTATCCAGTCCCACGGAAGATATTAGCGAACCGATGTTGCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTTGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGGCAGTATTAATTTGTCTTTCAGATTTCAAAAGATGAGTATc < 2:93836/250‑1 (MQ=255) cGGTGACTGACGTCGACAGCGGCGAAGCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCAGCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGTCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCtt < 1:400007/251‑1 (MQ=255) gacGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTg < 2:113338/249‑1 (MQ=255) aaTCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGAGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGcc < 2:89695/251‑1 (MQ=255) gTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAAGGACCATTGCGGCCAGAACGACCGAATTGAACGGCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGTTGAAGGTCAGGATGAATTTGTCGTTCAGATTTCAAAAGAGGAGTATCTCGATCGGCTCTTTGAAGATTTGGCCTTAcc < 2:38708/251‑1 (MQ=255) aCGGAAGAGATTAGCGAACCGATGTTTCATCAGGGGGGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACa < 2:433747/251‑1 (MQ=255) cGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCCTTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGAAGAGTATATTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACa > 2:453695/1‑250 (MQ=255) aGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAAcc < 1:409488/251‑1 (MQ=255) aatTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCGCCGCGTGCATCCGGGCAATGACCATGTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCTGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGGCAGGATGAAGTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTGTGAAGATTTGGCCTTACCGAATCTGAATCAAATCcaac < 2:128539/250‑1 (MQ=255) ttAGCGAACCGATGATTCATCAGGGGCGTGGCGGTCTCCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGGGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGGGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACcaaaa > 2:110701/1‑248 (MQ=255) aGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCaacaac < 2:330802/250‑1 (MQ=255) gcaCCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCCCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAAAGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCCGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAACCAAAACCAACAACGcc > 1:33237/3‑250 (MQ=255) gAACCGATCTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGGCCCCAGGGTGGCGGCGGAGGGTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTGCAAAAGATGAGTATCTTGATCTGCTCTTTGAATATTTGGCCTTACCGAATCTTAAACAAATCCATCAACGCCa < 2:438762/251‑1 (MQ=255) taatcaGGGGCGTGGCGTGCCGCGCCACCTGGTGCAACCGGGCAATGGCCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGTGGAGGTTCCGGCAGTGGTCAGTGCCAGGCCATCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACATCTCTAGCTGACCGaa < 2:267006/248‑1 (MQ=255) gTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATCACCCTTTCGTCCACAACGACCGACTTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCCGGGTGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCAt > 1:21555/1‑251 (MQ=255) ggTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCTTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAGCCAACAACGCCAGCTGACCGAATATAAAACGCATcgggc < 1:160009/251‑1 (MQ=255) gcgTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATCCCGCt > 1:438625/1‑251 (MQ=255) tGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGAGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTCCCGGcc > 2:301546/1‑250 (MQ=255) cATTTCATCCCGAACGACCGAATTGAACGTACCCCGGGTGGCGGCGGAGCTTCCGGCAGTGGTCAGACCCAGGCCAGCCAGGCTGGTGAAGGTCAGGATAAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACACCGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATCCCGCTAAAGACGTTCCGGcccaaat > 2:284492/1‑246 (MQ=255) cGTCCAGAACGGCCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCg < 1:333514/251‑1 (MQ=255) gTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGt < 2:104365/251‑1 (MQ=255) aTTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCa < 1:260959/232‑1 (MQ=255) aTTGAACGTCCACAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCAAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGATGACCGAATATAAAACGCATCGGGCGGGATATACCGCTAACGGCGTTCCGGCCAATTTCa > 2:260959/1‑232 (MQ=255) ggTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTgg > 1:417938/1‑250 (MQ=255) agatCAGGATGAATTTGTCTTGCAGATTTCAAAAAATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGATGACCGAATATAAAACGCATAGGGCGGGTTATACCGCTAAAGGAGATCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGAACAGCCATGAAAGCAGGAAAGCAACGGGAAcaccatgtacggaaag > 2:326926/4‑233 (MQ=255) aGGATGAATTTGTCTGTCAGATGTCAAAAGATGAGGATCTTGAGCTGCTCTTTGAAGATTTGGCCTTACCGAAGCTGAAAGAAACCCAACAGCGTCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGGTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCTCAGACATGACTTCAGGCAAGCGGCGGGAACTTCATGCACGGGAAGAGAATTTg < 2:294808/251‑1 (MQ=255) ggATGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTgg > 2:272103/1‑251 (MQ=255) aTGAATTTGTCTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATCTAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGcc > 1:183581/1‑251 (MQ=255) cTTTCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAAAGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCCTGCACTGGCAGAGAATTTGGACATCCTCAga > 1:356081/1‑249 (MQ=255) tttCAGATTTCAAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCa > 1:41520/1‑250 (MQ=255) cAGATTGCAAAAGATGAGTATCTTGATCTTCTCTTTGAAGAATTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATTCCGCTAACTGCGTGCCGGCCAATATCTGCGGTGTGCGTTCATTGCAGAACTCAGTGGCGCGACGCTCAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACa < 2:428551/250‑1 (MQ=255) | GTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACA > NC_000913/1869076‑1869555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |