Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,878	G→A	Q176* (CAG→TAG)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,878	0	G	A	97.4%	120.8 / NA	39	Q176* (CAG→TAG)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/11);  minor base C (1/0);  total (28/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACT  >  NC_000913/483644‑484120                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   tCACTTTCAGCAGGATTTTGCCGAAATCTTCAGTAGAGGTCAGACGCGTCTGAGCCATAATCGAGGAGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGGTCATCCAGATACGCATCGCGTACTGTGAACCGAACACCTAAAAATCACCCACGccc                                                                                                                                                                                                                                    >  2:290572/1‑251 (MQ=255)        cggcagTATTTTGCCGATCTCTTCAGAAGAGGTCAGACGCGTCTGAGAAATAATAGGGGCGTTAAGCTGTTGCCTTTTCCCGGGGGGCGTCCCCCCGGGCGGCCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTAc                                                                                                                                                                                                                             <  1:95946/249‑1 (MQ=255)                    gCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCAt                                                                                                                                                                                                                 >  1:8163/1‑251 (MQ=255)                     ccGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATACTAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCCGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAACGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGcctc                                                                                                                                                                                                                >  1:398684/1‑248 (MQ=255)                            ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCat                                                                                                                                                                                                          >  1:395702/1‑250 (MQ=255)                                aGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGAGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTcg                                                                                                                                                                                                     >  1:340716/1‑251 (MQ=255)                                                gTCTGCGCACTAATCGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGCACCACCGAGCTGACCCGCCGCAACCTCGGCGGTCTGCGCTTTGCTGGCGGTTTTGGCATCAACCGGCGTTAGCCGGAAATTCTTCAACACCTTCGGGTTCATCCAGATACGCATCGCGTACTGATAACCCGACACATCAACATCACCCACCCCCGGCGTACCGGTGATGGCCTCTTTCATTTTCTCCGCCACGTatttcgg                                                                                                                                                                                     >  2:363399/1‑245 (MQ=255)                                                              agagGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAAACGAACAACTAAACCTCCCCCACGCCCCACGTACGGCTGAGGGCATCTTTCATATTAGCCGCCCCGTAGTCGgaggaatactcccgcg                                                                                                                                                                       >  2:343408/1‑238 (MQ=255)                                                                  gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGGTAGCTGGAATTTGTTCCGCCCATTCGGGTTCATCCAGATACGCATCGCGGACTGTGAACCGTACAACTAAACCTCACCCACGCCCGACGTACGGCCGGTGGCATCTTTCCTATTCGCCGCCACTTAGGCGGAGATATCCTCCTGCGTCAt                                                                                                                                                                   >  2:202812/1‑251 (MQ=255)                                                                                gCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCggggg                                                                                                                                                     >  1:257748/1‑248 (MQ=255)                                                                                             ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCCTTCGGGTTCATCCAGCTACGCATCGCGTACTGTGCACCGAACAACTAAACATCACCCACGCCCTACGTACGGCTGATGGCATCTTTCATATTCGCAGCCACGTAGTAGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGGTAACGCCgcc                                                                                                                                        >  2:327545/1‑249 (MQ=255)                                                                                             ggcgTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGGTCATCCAGATACGCCTCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCAGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGAc                                                                                                                                        >  1:142567/1‑251 (MQ=255)                                                                                                   ccaccGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCAt                                                                                                                                  >  1:63410/1‑251 (MQ=255)                                                                                                   acaccggagtcacacgccgtagccTGGGCGGTGTGCGTTTTGATGGCGGTAATGACTGCAACCGGTGTTAGCTGGAAGTGGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACGGTGAACCGAACAACTAAACATCACCCATGCCCTACGTTCGGCTGATGGCATCTTTCATATGCGCCGCTCCGTAGACGGAGATATCCTCCTGCTTCATGGGGCCATCGGTGTTGAGAACGCCGACCACCAt                                                                                                                                  <  2:353130/229‑1 (MQ=255)                                                                                                       cGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCCACCCGCGTTAGCTGGAATTTGTTCCGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACCTCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACCGAGTCGGAGATATCCGCCTGCGACATGGTGCCATCGGTGTTGATACAGCAGACAACAATCa                                                                                                                                >  2:235831/1‑249 (MQ=255)                                                                                                           cTGACCCGCCGAAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGGTAGCTGGACTTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGCACTGTGAACCGAAACACTAAACATCACCCCCGCCCGACGTACGGCCGATGGCATCTTTCATATTCCCCGCCACGTCGACGGAGCTATCCTCCTGCGTCATGGGTCCCTCGGGGTTGGTACCGCCGACAAccctccggcagc                                                                                                                          >  2:389723/1‑241 (MQ=255)                                                                                                             tccccGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCGTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAgc                                                                                                                          <  1:306093/247‑1 (MQ=255)                                                                                                                     gcAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGgatg                                                                                                                 >  1:328936/1‑250 (MQ=255)                                                                                                                     gcAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGGTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCCTCTTTCATATTCGCCGCACCGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACACCCCTCAGGAAGCTGCGGgatg                                                                                                                 >  2:429288/1‑250 (MQ=255)                                                                                                                            gggCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTtct                                                                                                           <  1:323719/249‑1 (MQ=255)                                                                                                                                     gcgcTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTc                                                                                                   <  1:351273/248‑1 (MQ=255)                                                                                                                                        cTTTGATGGCGGTAATGACATCAACCGGCGTTAGCGGGAATCTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGCACACCTAAACATCACCCACGCCCGCCGTACGGCTGATGGCATATTTCATATTCGCCGCCACGTAGTCGGAGATATCATCCTGCGTCATGGTGCCATCGGTGTTAATAAAGCCGCCAACCATCAGGAAGCTGCTGGACGCTTTCTCAACGCTCACCCCt                                                                                             >  1:33616/1‑251 (MQ=255)                                                                                                                                          ttGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCtt                                                                                            <  1:155645/250‑1 (MQ=255)                                                                                                                                          ttGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTtg                                                                                           >  1:327202/1‑251 (MQ=255)                                                                                                                                                   gTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAAc                                                                                  <  2:395702/251‑1 (MQ=255)                                                                                                                                                                  ggCGTTAGCTGGAATTTGTTCAGCTCATCCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGAGCCATCGGAGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGCACTTCTTGCGgcagcac                                                                   >  2:149760/1‑250 (MQ=255)                                                                                                                                                                            gtaatgtggccAGCTCTTTCGGGTTCATCCAGATACGCATCGCGTACTGTGATCCGAACAACTAAACATCGCCCACGCCCTACGTACGGCTGATTGCATCTTTCATATTCTCCTCCACGTAGTCGTAGAGATCCTCCTGCGTCATGGTGCCATCGGTGTTTATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGTCATCg                                                           <  2:246940/239‑1 (MQ=255)                                                                                                                                                                                     tCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt                                                >  1:273949/1‑251 (MQ=255)                                                                                                                                                                                         cTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGCACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt                                            >  2:435274/1‑251 (MQ=255)                                                                                                                                                                                           cATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTc                                          >  1:395999/1‑251 (MQ=255)                                                                                                                                                                                              tCGGGTTCATCCAGATACGAATCGCGTACTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTACAGTTTGTTCTGc                                       >  2:447908/1‑251 (MQ=255)                                                                                                                                                                                               cggggtaaTCCAGATACGCATCGCGTCCTGTGAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGACGCCACGTAGTCGGAGATATCATCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAAACATCAGGAAGCTGCTGGCTGATTTCTCAACGCTAACCCCTTGATGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCCGTTTGTTCTGcc                                      >  2:268485/8‑250 (MQ=255)                                                                                                                                                                                                gggTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTAAACATCGCCCGCGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTTCTGCTGAACATCTTTCGTCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAc                                     <  1:426430/251‑1 (MQ=255)                                                                                                                                                                                                gggTTCATCCAGATAAGCATCGCGTACTGTGAACCGAACAACTACAAATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGCGATATCCTCCTGCGTCAAGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGCTGATTTCTCAACGCTCACCCCTTGCTGATGAACTTCATGAGACAGCAACGGCATCGCAATCTGCAGTTTGTTCTGCAc                                     >  1:369683/1‑251 (MQ=255)                                                                                                                                                                                                                    cgcgTACTGTGAACCGAACAACTAAACAGCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg                 <  1:268485/251‑1 (MQ=255)                                                                                                                                                                                                                            gtgAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt          >  2:380539/1‑250 (MQ=255)                                                                                                                                                                                                                              gAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCACCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAcc       >  1:197208/1‑251 (MQ=255)                                                                                                                                                                                                                              gAACCGAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAcc       >  1:256366/1‑251 (MQ=255)                                                                                                                                                                                                                                   gAACAACTAAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt  <  1:379385/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   TCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGACCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTGAACCGAACAACTGAACATCACCCACGCCCGACGTACGGCTGATGGCATCTTTCATATTCGCCGCCACGTAGTCGGAGATATCCTCCTGCGTCATGGTGCCATCGGTGTTGATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACT  >  NC_000913/483644‑484120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑