breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 42,108 G→A intergenic (‑177/‑295) caiT ← / → fixA L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
RA 47,202 C→T intergenic (+64/‑44) yaaU → / → kefF putative transporter YaaU/regulator of KefC‑mediated potassium transport and quinone oxidoreductase
RA 60,647 C→T G873D (GGC→GAC)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 65,588 G→A L65L (CTG→TTG)  polB ← DNA polymerase II
RA 82,705 C→T Q275Q (CAG→CAA leuA ← 2‑isopropylmalate synthase
RA 101,074 C→T P104S (CCG→TCG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 106,736 C→T T60T (ACC→ACT lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 107,182 G→A G209D (GGC→GAC)  lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 122,761 C→T H224Y (CAT→TAT)  pdhR → DNA‑binding transcriptional dual regulator PdhR
RA 159,351 G→A R254R (CGC→CGT gluQ ← glutamyl‑Q tRNA(Asp) synthetase
RA 164,214 C→T P704S (CCT→TCT)  hrpB → RNA‑dependent NTPase HrpB
RA 190,836 C→T intergenic (+237/‑21) rpsB → / → tsf 30S ribosomal subunit protein S2/protein chain elongation factor EF‑Ts
RA 229,552 C→T S129F (TCC→TTC)  dkgB → methylglyoxal reductase DkgB
RA 237,657 C→T S108F (TCC→TTC)  yafT → lipoprotein YafT
RA 239,186 G→A intergenic (‑450/+233) ykfM ← / ← yafV uncharacterized protein YkfM/2‑oxoglutaramate amidase
RA 257,588 C→T C354C (TGC→TGT frsA → fermentation‑respiration switch protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 258,921 IS1 (+) +9 bp pseudogene (246‑254/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
RA 281,675 C→T Q103Q (CAG→CAA yagA ← integrase core domain‑containing protein YagA
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 296,399 G→A A233V (GCT→GTT)  intF ← putative phage integrase
RA 377,492 G→A intergenic (+181/+43) yaiL → / ← frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
RA 385,137 G→A intergenic (‑521/‑95) yaiS ← / → tauA putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
MC JC 498,522 Δ406 bp coding (468‑873/963 nt) hemH → ferrochelatase
RA 549,527 G→T G391W (GGG→TGG)  ylbE → DUF1116 domain‑containing protein YlbE
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 790,832 Δ1 bp coding (198/1047 nt) galT ← galactose‑1‑phosphate uridylyltransferase
JC JC 791,653 IS1 (–) +8 bp coding (396‑403/1017 nt) galE ← UDP‑glucose 4‑epimerase
RA 915,224 C→T intergenic (‑367/+128) lysO ← / ← aqpZ L‑lysine/thialysine exporter/water channel AqpZ
RA 920,904 C→T I186I (ATC→ATT macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 991,161 C→T F180F (TTC→TTT pepN → aminopeptidase N
RA 1,001,251 A→C Q679P (CAA→CCA)  elfC → putative fimbrial usher protein ElfC
RA 1,006,070 C→T T40I (ACT→ATT)  zapC → cell division protein ZapC
RA 1,031,880 C→T intergenic (‑168/‑259) serT ← / → hyaA tRNA‑Ser/hydrogenase 1 small subunit
JC JC 1,034,134 IS30 (–) +2 bp coding (881‑882/1794 nt) hyaB → hydrogenase 1 large subunit
RA 1,057,502 C→T R151R (CGG→CGA torR ← DNA‑binding transcriptional dual regulator TorR
RA 1,148,972 C→T Q72* (CAG→TAG)  fabH → 3‑oxoacyl‑[acyl carrier protein] synthase 3
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,220,316 C→T intergenic (+1115/+1989) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,287,294 C→G noncoding (35/85 nt) tyrV ← tRNA‑Tyr
JC JC 1,293,326 IS1 (–) +9 bp intergenic (‑404/‑193) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,302,204 Δ1 bp coding (1023/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,302,207 +A coding (1026/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,407,078 T→A T240S (ACT→TCT)  dgcM ← diguanylate cyclase DgcM
RA 1,495,346 C→T A20V (GCC→GTC)  ydcJ → DUF1338 domain‑containing protein YdcJ
JC JC 1,545,555 IS5 (–) +4 bp coding (157‑160/957 nt) yddK ← leucine‑rich repeat domain‑containing protein YddK
RA 1,556,573 C→T intergenic (‑293/‑52) bdm ← / → osmC biofilm‑dependent modulation protein/osmotically inducible peroxiredoxin OsmC
JC JC 1,590,490 IS2 (+) +5 bp intergenic (‑489/+360) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,607,310 C→T intergenic (+21/‑36) lsrG → / → tam (4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase/trans‑aconitate 2‑methyltransferase
RA 1,638,483 G→A P62L (CCT→CTT)  cspI ← cold shock‑like protein CspI
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,821,148 G→A P23S (CCA→TCA)  chbC ← N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
RA 1,829,887 G→A A293A (GCC→GCT astA ← arginine N‑succinyltransferase
RA 1,882,226 C→T N105N (AAC→AAT dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,948,964 G→A A520V (GCT→GTT)  aspS ← aspartate‑‑tRNA ligase
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,042,171 G→A intergenic (+146/‑197) zinT → / → yodB metal‑binding protein ZinT/putative cytochrome b561 YodB
RA 2,044,642 T→C intergenic (+18/‑296) asnT → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,136,928 G→A P106S (CCA→TCA)  wza ← outer membrane polysaccharide export protein Wza
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC 2,176,206 (CAGCACTTTGC)1→2 coding (116/1263 nt) gatZ ← putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
RA 2,199,440 G→A intergenic (+2142/‑839) yehF → / → yehI PF05406 family protein YehF/DUF4132 domain‑containing protein YehI
JC JC 2,238,689 IS1 (+) +8 bp coding (594‑601/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
RA 2,298,327 G→A A131V (GCC→GTC)  napB ← periplasmic nitrate reductase cytochrome c550 protein
JC JC 2,320,237 IS2 (+) +5 bp coding (195‑199/1827 nt) atoS → sensor histidine kinase AtoS
JC JC 2,375,828 IS186 (+) +4 bp coding (132‑135/963 nt) menC ← o‑succinylbenzoate synthase
RA 2,413,411 C→T intergenic (‑279/‑59) yfbV ← / → ackA PF04217 family membrane protein YfbV/acetate kinase
RA 2,414,829 A→G E28G (GAA→GGA)  pta → phosphate acetyltransferase
JC JC 2,415,251 IS30 (–) +2 bp coding (505‑506/2145 nt) pta → phosphate acetyltransferase
RA 2,440,756 C→T A284T (GCG→ACG)  fabB ← 3‑oxoacyl‑[acyl carrier protein] synthase 1
JC JC 2,468,298 IS3 (+) +3 bp coding (85‑87/921 nt) yfdH → bactoprenol glucosyl transferase
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,599,947 G→T L14F (TTG→TTT gcvR → putative transcriptional regulator GcvR
RA 2,612,191 G→T G98* (GGA→TGA)  hyfR → DNA‑binding transcriptional activator HyfR
RA 2,785,386 G→A intergenic (‑375/+376) ypjC ← / ← ileY DUF5507 domain‑containing protein YpjC/tRNA‑Ile
RA 2,816,658 G→A P95S (CCC→TCC)  yqaA ← DedA family protein YqaA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,033,927 G→A F416F (TTC→TTT lysS ← lysine‑‑tRNA ligase
RA 3,075,092 C→T R35H (CGC→CAC)  fumE ← fumarase E
RA 3,152,494 G→T A87S (GCG→TCG)  metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
JC JC 3,350,556 IS1 (–) +8 bp intergenic (‑97/‑14) elbB ← / → arcZ low activity glyoxalase ElbB/small regulatory RNA ArcZ
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,398,384 C→T intergenic (‑6/+3) yhdE ← / ← mreD nucleoside triphosphate pyrophosphatase YhdE/cell shape determining protein MreD
JC JC 3,429,986 IS3 (–) +5 bp :: +T coding (34‑38/258 nt) yrdB ← DUF1488 domain‑containing protein YrdB
RA 3,456,451 C→T N25N (AAC→AAT gspD → Type II secretion system protein GspD
RA 3,465,154 C→T L25F (CTC→TTC)  gspM → Type II secretion system protein GspM
RA 3,471,447 C→T A690T (GCG→ACG)  fusA ← elongation factor G
RA 3,496,751 G→A W67* (TGG→TGA nirD → nitrite reductase (NADH) small subunit
RA 3,507,330 G→A L3L (CTG→TTG)  yhfT ← uncharacterized protein YhfT
RA 3,510,905 G→A G24G (GGC→GGT yhfX ← putative PLP‑binding protein YhfX
RA 3,549,497 G→A P192S (CCG→TCG)  malQ ← 4‑alpha‑glucanotransferase
RA 3,560,443 C→G intergenic (+577/+179) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,631,612 IS5 (+) +4 bp coding (976‑979/1623 nt) yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,641,276 C→T P139S (CCG→TCG)  dtpB → dipeptide/tripeptide:H(+) symporter DtpB
RA 3,689,145 G→T T10K (ACG→AAG)  bcsC ← cellulose synthase outer membrane channel
RA 3,696,157 G→T P10H (CCT→CAT)  yhjR ← PF10945 family protein YhjR
RA 3,703,531 G→T Y106* (TAC→TAA dppD ← dipeptide ABC transporter ATP binding subunit DppD
RA 3,707,883 G→A intergenic (‑178/+733) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,888,620 C→T intergenic (+111/‑110) tnaC → / → tnaA tnaAB operon leader peptide/tryptophanase
RA 3,901,161 C→T G354S (GGT→AGT)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,991,903 C→T L251F (CTC→TTC)  cyaA → adenylate cyclase
RA 4,128,813 Δ2 bp coding (142‑143/1161 nt) metB → O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
RA 4,171,788 G→T intergenic (+32/‑269) rrfB → / → murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,284,602 A→C Y101D (TAT→GAT)  actP ← acetate/glycolate:cation symporter
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,359,314 G→A Q240* (CAG→TAG)  cadB ← lysine:cadaverine antiporter
RA 4,373,898 G→A L113F (CTT→TTT)  yjeJ ← protein YjeJ
RA 4,380,292 2 bp→A coding (225‑226/735 nt) frdB ← fumarate reductase iron‑sulfur protein
RA 4,387,000 G→A L791L (CTG→TTG)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,387,978 C→T A465T (GCC→ACC)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,429,994 G→A G44E (GGG→GAG)  cycA → D‑serine/alanine/glycine/:H(+)symporter
RA 4,436,956 2 bp→TT coding (202‑203/741 nt) cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,503,361 C→T G24E (GGG→GAG)  insG ← IS4 family putative transposase
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,549,037 T→C V77A (GTC→GCC)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,585,480 G→A Q428* (CAA→TAA)  hsdR ← type I restriction enzyme EcoKI endonuclease subunit
RA 4,602,509 C→T W117* (TGG→TAG)  yjjP ← putative succinate exporter YjjP
RA 4,616,669 G→T L133I (CTA→ATA)  yjjI ← DUF3029 domain‑containing protein YjjI
MC JC 4,628,621 Δ6,226 bp ettA[rob] ettA, slt, trpR, yjjX, ytjC, [rob]
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 323451 362203 38753 33 [0] [0] 21 [ykgF]–[lacY] [ykgF],ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,[lacY]
* * ÷ NC_000913 1196298 1211437 15140 5 [4] [4] 5 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1263890 1264273 384 5 [4] [4] 5 hemA glutamyl‑tRNA reductase
* * ÷ NC_000913 1907645 1907878 234 32 [0] [0] 19 yobF/yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
* * ÷ NC_000913 3423887–3424231 3424423–3424239 9–537 5 [4] [4] 5 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3942258–3943393 3943456–3943399 7–1199 5 [4] [4] 5 [rrsC]–[gltU] [rrsC],[gltU]
* * ÷ NC_000913 4166499–4168220 4168506–4168477 258–2008 5 [4] [4] 5 rrsB–gltT rrsB,gltT
* * ÷ NC_000913 4542159 4547064 4906 25 [0] [0] 33 [fimE]–[fimD] [fimE],fimA,fimI,fimC,[fimD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)33 (1.320) 31/492 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 323450 0 (0.000)coding (1113/1428 nt) ykgF putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
* ? NC_000913 = 258675NA (NA)21 (0.840) 19/492 0.4 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 = 56677427 (1.080)11 (0.440) 8/488 1.7 34.5% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 15 (0.600)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 19076440 (0.000)32 (1.280) 29/492 0.1 100% intergenic (‑53/+617) yobF/yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1907879 =0 (0.000)19 (0.760) 18/492 0.5 100% intergenic (‑288/+382) yobF/yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1978503 =NA (NA)33 (1.320) 31/492 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4547065 = 0 (0.000)coding (1970/2637 nt) fimD type I fimbriae usher protein
* ? NC_000913 3583428 =NA (NA)25 (1.000) 23/492 0.2 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4542158 0 (0.000)coding (122/597 nt) fimE regulator for fimA