Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,661	A→T	intergenic (+503/‑108)	nth → / → dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,661	0	A	T	100.0%	26.7 / NA	10	intergenic (+503/‑108)	nth/dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/5);  total (5/5)

CACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACG  >  NC_000913/1712465‑1712869                                                                                                                                                                                                     |                                                                                                                                                                                                                 cACTAGTAAAACCGCAAAATAGCAGAACATATCGCCGTTAAGCCATCCCCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGtttttt                                                                                                                                                               <  1:179039/248‑1 (MQ=255)          aaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAAcgcg                                                                                                                                                   >  1:82625/1‑251 (MQ=255)           ccttccgatcgccaaaaaatacccccGTTAACCCACCCCCCCCCCCTCAGTGCAGTAAAAAAATCTGCCGTTACGTTTTTTAAAAAATTTACCGCTGGATAACATTCCCCGGATTGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCga                                                                                                                                                  <  2:98705/227‑1 (MQ=255)                      gCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCga                                                                                                                                                  >  1:98705/1‑240 (MQ=255)                            catatggaggTTAAGTCATCAACCCCCTCTCAGAGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACACTGGATAACATTTCCCGGAATGGTTGAATTACCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTa                                                                                                                                  >  2:122095/10‑250 (MQ=255)                                                                                       tttttGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGc                                                                      >  1:80767/1‑251 (MQ=255)                                                                                                    tAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACg                                                         >  1:90476/1‑251 (MQ=255)                                                                                                                                                      atGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATgg       <  2:29363/251‑1 (MQ=255)                                                                                                                                                          aaCAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGaa    <  1:154464/250‑1 (MQ=255)                                                                                                                                                           aCAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGTACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCTGTTTGAACGCTTTCAAACAACCGAAGGCGTGCTATCTCATCTTCTCGATTGAGTGATGAGAACg  <  2:17157/251‑1 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                 CACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCTCAGTTATATGTAACAGATTATTACAAAGGACTTGTCTGAAAGTGCAAGATAGTGAACATTACCTGCCGTTTCCCCTCCCACTATAACAATTGCGCGTATGTTTTTTATACATAACGCGAGAAAGCACCCCCGTTAATATGGGATGTAAAAAAAGAGGTAAAAGTGTCCACTGCAAACCAAAAACCAACTGAAAGCGTCAGTTTGAACGCTTTCAAACAACCGAAGGCGTTCTATCTCATCTTCTCGATTGAGTTATGGGAACG  >  NC_000913/1712465‑1712869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑