Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G96.4% 82.8 / NA 28intergenic (+589/+167)rtcR/glpGDNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (7/20);  minor base T (0/1);  total (7/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560209‑3560696
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
tcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATCGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGcgca                                                                                                                                                                                                                                                 <  1:130991/250‑4 (MQ=255)
      aCCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCa                                                                                                                                                                                                                                           <  1:175713/250‑1 (MQ=255)
           cAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                                                                                                                                     <  1:199096/251‑1 (MQ=255)
           aacggtgtAAGCAATGTTGAGATTTTTGGTGACACGGCGCAATTTGCTGTGATGGGGCGGTGCG‑TGCGTTCCCGCTTCGGGCGTGG‑GGCCGTGAGCGATAAACTGCGTCGTGCCATTGGTGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATTATGGCTCAGGAGCAGGtttt                                                                                                                                                                                                                                     <  2:218003/247‑1 (MQ=255)
                 gtTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCGATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCtacg                                                                                                                                                                                                                               >  1:230495/1‑248 (MQ=255)
                      cAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                          >  1:117030/1‑251 (MQ=255)
                       aacgagcagaTTGGTGATGACAAGGCGAAAATGGCGGGGATTGAGCATGGCG‑GGCGATACCTCTTCCGG‑GTGGGTGCAGATAGGGATAATCGGTGTCGAGCCAGTGGGAGTGTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGtttttttccggcagcg                                                                                                                                                                                                                         <  2:37355/244‑13 (MQ=255)
                             gagaTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                    <  1:232558/250‑1 (MQ=255)
                                      ggTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                                                                                                                                          <  1:111055/251‑1 (MQ=255)
                                                   aaaTTGCGGTGATTGAGTAG‑GCGGTGCGCCACCGCTTCCGGCGGTG‑TGCCGATCTCGATAAACAGCTGCGAGCCATTGGGGAGTGGCTCCGCCTCTGTGCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAGCGGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCATCTCAGTGAGGTCGCGGGGAATAGTCTGCgg                                                                                                                                                                                             <  2:232172/251‑1 (MQ=255)
                                                        gCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGa                                                                                                                                                                                        <  1:101542/251‑1 (MQ=255)
                                                        gCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑GGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                         <  1:126332/250‑1 (MQ=255)
                                                                 ggggcagggcttgcacgaCCGGTTCAGGCGGGG‑TGCCGATATCGATAAACAGCGTGGAGACATGGGGGATTTGGTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                                                                                                                                <  2:261/234‑1 (MQ=255)
                                                                 gAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGA‑GGGAGGGCCCCCGCGCCATGATGGGGCAGGGTTCGGGTTTGCTCCGCCCGCCTATTGAGGGCGCGGGGAAGAATCTGGGGGCTGAAGgaagaatg                                                                                                                                                                              >  1:46895/1‑245 (MQ=255)
                                                                     aGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATAGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACTGGCAGGCAGCGCCGCAGCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGctc                                                                                                                                                                           >  2:104460/1‑251 (MQ=255)
                                                                                    cGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                                                                                                                             <  1:11568/251‑1 (MQ=255)
                                                                                         ccGGCGGGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                                                                                                                                        <  1:154309/251‑1 (MQ=255)
                                                                                                 g‑tgCCGATATCGATAAACCGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                                >  2:186333/1‑251 (MQ=255)
                                                                                                    gCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc                                                                                                                                               <  1:188755/250‑1 (MQ=255)
                                                                                                                       gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                                           >  2:173961/1‑251 (MQ=255)
                                                                                                                                      aTTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTTGTTCGGGCTGGGGGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCAGCGCGATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                            <  2:166447/251‑1 (MQ=255)
                                                                                                                                                          ggCGATGCGCGCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTTTTTCATTta                                                                                          <  2:227135/249‑1 (MQ=255)
                                                                                                                                                                  gcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                                <  2:82996/251‑1 (MQ=255)
                                                                                                                                                                                                 aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                                 <  1:225111/251‑1 (MQ=255)
                                                                                                                                                                                                   cGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGaagc                                               <  2:3973/251‑3 (MQ=255)
                                                                                                                                                                                                                                ggCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa                  <  1:121479/251‑1 (MQ=255)
                                                                                                                                                                                                                                          cagcGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAt        >  1:110085/4‑251 (MQ=255)
                                                                                                                                                                                                                                             cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgtg     <  1:21229/251‑1 (MQ=255)
                                                                                                                                                                                                                                                cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc  <  2:129403/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 
TCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCG‑TGCGCTACCGCTTCCGGCGTGG‑TGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560209‑3560696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑