Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,298,327	G→A	A131V (GCC→GTC)	napB ←	periplasmic nitrate reductase cytochrome c550 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,298,327	0	G	A	100.0%	74.3 / NA	24	A131V (GCC→GTC)	napB	periplasmic nitrate reductase cytochrome c550 protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/14);  total (10/14)

GCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTG  >  NC_000913/2298089‑2298562                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            gCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGt                                                                                                                                                                                                                                 >  1:123296/1‑251 (MQ=255)            cGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCCCCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGGATCGACCTGCGGTACGTGACATTGcaga                                                                                                                                                                                                                      <  1:186000/251‑1 (MQ=255)             ggTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGcagac                                                                                                                                                                                                                     >  1:16049/1‑251 (MQ=255)             ggTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGcagac                                                                                                                                                                                                                     <  1:190753/251‑1 (MQ=255)                     cTTTTTCCATCCCGGTGTTAACGCCACCCGAGACAAGGATTCCGCCAACACACCCGATAAACAGCCGCGTCCCCAGCGCCCCACCACTAGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGAACAGGCTTACGCTCAGCATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGACGGGGTACA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCACACAGAAATa                                                                                                                                                                                                              >  2:112737/1‑250 (MQ=255)                           ccATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAacgacg                                                                                                                                                                                                        <  2:17873/250‑1 (MQ=255)                           ccATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGAcgc                                                                                                                                                                                                       <  1:30366/251‑1 (MQ=255)                            cATCCCGGTGTTAAAGCCGCCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGAcgcg                                                                                                                                                                                                      <  2:91443/251‑1 (MQ=255)                                                          gATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGc                                                                                                                                                                        >  1:166925/1‑251 (MQ=255)                                                               cgccAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTcc                                                                                                                                                                   >  1:209392/1‑251 (MQ=255)                                                                          ccGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGt                                                                                                                                                        <  1:83481/251‑1 (MQ=255)                                                                            gATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGt                                                                                                                                                        >  1:34557/1‑249 (MQ=255)                                                                                  cagcagCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGa                                                                                                                                                <  1:50751/251‑1 (MQ=255)                                                                                                    cAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAgtgg                                                                                                                              <  2:103165/251‑1 (MQ=255)                                                                                                                ggTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTGCCCATAATAACCTCTTAGTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGAGGGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACATAAATAACGACGCGGAGCCACGTCCTCGGCCACTTTGGCGTGGCGTTCCTTAAAGTTAGTATGATAGAGACGCTGCGCGCGATTGGTGCGATAGCttt                                                                                                                  <  2:120885/251‑1 (MQ=255)                                                                                                                          caTTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTg                                                                                                        <  2:16049/251‑1 (MQ=255)                                                                                                                               ccACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACt                                                                                                   <  2:34557/251‑1 (MQ=255)                                                                                                                                                      gCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTc                                                                            >  2:217689/1‑251 (MQ=255)                                                                                                                                                          aCGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTACCCTTTTGATGGG‑TAAACGGTATTCCCCACGATTCGCGAGGTCTCGACCTCCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCAGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATCGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGACTTGGTCGtt                                                                         >  1:19065/1‑250 (MQ=255)                                                                                                                                                                 gAATGTCCCATAATATCCTCTTATTTCCCGTAACCTTTTGATGGTGTAAA‑GGTATGCCCCACGATTGGCGCGATATCGACCTGCGGTACGTGACATGGCAGACAGAAATAACGGCGCGGAGCCACTGCCGCGCCCGCTTGGCCGTCGCTGTCCATAAAGTGAGTAGGGCTGATACGCGGCTCGCGATTGGTGGGAAAGGGATTGTCACGGGGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATa                                                                 <  1:25417/251‑1 (MQ=255)                                                                                                                                                                     ttCCCATAATAACCTCGTATTTCCCGTAACCGTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACTCCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAAcc                                                              <  2:31917/250‑1 (MQ=255)                                                                                                                                                                                    cgtATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGTGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCgg                                               <  2:123296/248‑1 (MQ=255)                                                                                                                                                                                                       tGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGCTAACCTTCAACGCTATGCGTGATCATTGGCGGCTGATTcc                           >  2:138262/1‑250 (MQ=255)                                                                                                                                                                                                                                 aTTGGCGCGGTATCGACCTGCGGTACGTGACATTGCAGACAGAAATAACGAAGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTg  >  1:19961/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            GCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCTGGGGGTACGCCACCATTTCCACAGGCGCTTAATCAGACCAGGCTTACGGTCAGAATTTCCCATAATAACCTCTTATTTCCCGTAACCTTTTGATGGGGTAAA‑GGTATTCCCCACGATTGGCGCGGTATCGGCCTGCGGTACGTGACATTGCAGACAGAAATAACGACGCGGAGCCACTTCCGCGCCCACTTTGCCGTCGCTGTCCATAAAGTGAGTAGGACTGATACGCGGCGCGCCAGTGGTGCGATAGCTTTCGACACCGTGGCACTGCAAGCAGCGATTGGTATTGGTCGTTACCTGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTG  >  NC_000913/2298089‑2298562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑