Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,901,161	C→T	G354S (GGT→AGT)	bglH ←	carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,901,161	0	C	T	100.0%	65.3 / NA	19	G354S (GGT→AGT)	bglH	carbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

TGTTTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGC  >  NC_000913/3900928‑3901400                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                tGTTTCGTAGTTATATATATCGGTACCGAAGGAGTAAACAATCGCGTTAGCGACAAGGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGCCTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGGTGAAGCCCCTTTTATCAAATTTCTGc                                                                                                                                                                                                                                 <  2:224452/250‑1 (MQ=255)    ttCGTAGCTATATATCTCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGACGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGtt                                                                                                                                                                                                                              >  2:194116/1‑250 (MQ=255)          gCTATATATATCGTTACCGAAGGAGTAAACAATCCAGCTAGCTACAATGAAATGACCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGCAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGAtg                                                                                                                                                                                                                       >  1:225744/1‑251 (MQ=255)               atatatCGTTAACGAAGGAGTAAACAATCGCGTTAGCTACAATGAACTGATCGACAATCTAGGCTTCGCCCTGCGAAGTAAGACGTACAGCTGTTCCGCCGGTGTGATCCCCATAATAAAGACCATTAAAGGTGGCAAATGGACTTGCGCCAGCATAACGGCCAAAGTTCCTGGCGATAGAGTTATTCGAGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGca                                                                                                                                                                                                                   >  1:159469/1‑249 (MQ=255)                                          tCGCGTTAGCTACAATGAAATGATCGCCAATATCGGCTTCACCCTGCGAAGTCCGAAGTACCGCTGTTCCGCCGGTGCGATCACCATAATCACGACCCTTACAGGTGGCACATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGACTTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCata                                                                                                                                                                                        >  1:241338/1‑250 (MQ=255)                                                        atgaaatgaTCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATc                                                                                                                                                                         <  1:232829/251‑1 (MQ=255)                                                         tgaaatgaTTGCCAATATAGTCTTCGCCCTGTGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCCGTCATAATACCCGTTATTATc                                                                                                                                                                         <  2:118547/250‑1 (MQ=255)                                                                     cAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCtttt                                                                                                                                                            >  1:125277/1‑251 (MQ=255)                                                                     cAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGAGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCtttt                                                                                                                                                            >  1:31083/1‑251 (MQ=255)                                                                         ataGGCTTCGCCCTGCGAGGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCg                                                                                                                                                        <  1:249083/251‑1 (MQ=255)                                                                                 cGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCtt                                                                                                                                                >  2:60804/1‑251 (MQ=255)                                                                                   cccTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTc                                                                                                                                              <  1:237045/251‑1 (MQ=255)                                                                                           aGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCg                                                                                                                                      >  2:210004/1‑251 (MQ=255)                                                                                                                      gtgATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAgg                                                                                                           >  2:8050/1‑251 (MQ=255)                                                                                                                                                                         aTAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAAtggtattgg                                                        <  1:52455/251‑1 (MQ=255)                                                                                                                                                                                  aaaGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTg                                                                                                                                                                      >  2:42060/1‑132 (MQ=255)                                                                                                                                                                                  aaaGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTg                                                                                                                                                                      <  1:42060/132‑1 (MQ=255)                                                                                                                                                                                             cGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTAGTGGTATTAATCTGCTgtttgttt                                    <  2:246502/251‑1 (MQ=255)                                                                                                                                                                                                                                tttaaGCCACTTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATCCCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTcgc  <  2:77065/247‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                TGTTTCGTAGCTATATATATCGTTACCGAAGGAGTAAACAATCGCGTTAGCTACAATGAAATGATCGCCAATATAGGCTTCGCCCTGCGAAGTCAGACGTACCGCTGTTCCGCCGGTGTGATCACCATAATAACGACCATTAAAGGTGGTAAATGGACTTGCGCCAGCATAACGGCCAAAGTTACTGGCGATAGAGTTATTCGCGACCAGGAAGGAGAATTCGTTGAAGCCACCTTTATCAAATTTCTGCGTTAAAGATGTGCCAAACATCCAGGTATCTTTCCAGTCATAATACCCGTTATTATCCTGATTATCTTTTTCCGATGCGCTTTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGC  >  NC_000913/3900928‑3901400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑