Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,092	C→T	R35H (CGC→CAC)	fumE ←	fumarase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,092	0	C	T	96.9%	99.7 / NA	32	R35H (CGC→CAC)	fumE	fumarase E
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/11);  minor base A (1/0);  total (21/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AATCCCAGGTCATGTCATCGGCAAA‑TCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCC  >  NC_000913/3074853‑3075337                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aaTCCCAGGTCATGTCATCGGCAAA‑TCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCAGCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTAGCCCCTgg                                                                                                                                                                                                                                            <  1:21227/251‑1 (MQ=255)    cccAGATCATGTCAT‑GGCAAAATCCGGGCGCTTCATCCAGTTCGTTCAGGTAATGCCCCAACTGGAAAAAATGCGTGATATCGGCGTACAACCATTTATTCATTTTCACCAACGCATAAATCAAACGAAGTGCAACATCAATATCGTCAAGCGTGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGAAGGTAGAAACTGGTGTATccccctg                                                                                                                                                                                                                                            >  2:229740/1‑245 (MQ=255)     acaggtaaggtagtCGGCAAA‑TCCGGGCGTTTCATCCTGTACGTTCAGGTAATGCCAGAACGGGGAAAAATGCGTGATATTGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATGAAACGCAGTGCAAGATCAAGATCGGCAAGCGGGGCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGAGCTCTTCATCGTTAAGGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCa                                                                                                                                                                                                                                        <  1:241957/238‑1 (MQ=255)              gtcatCGGCAAA‑TCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTa                                                                                                                                                                                                                                <  1:83891/250‑1 (MQ=255)                                  gTTTCCTCCTGTTCGTTCCGGTACTGCCAGACCTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACCTCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGATTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCACTGGAGCAAAATGGAATGCGCAGTTTTCATAAATg                                                                                                                                                                                                             >  2:230272/1‑250 (MQ=255)                                  gTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGa                                                                                                                                                                                                            <  2:203691/251‑1 (MQ=255)                                                        aaTGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATcc                                                                                                                                                                                      >  1:194513/1‑251 (MQ=255)                                                                   tGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCa                                                                                                                                                                             >  1:94202/1‑249 (MQ=255)                                                                     ggAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACACACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCacgc                                                                                                                                                                          >  1:151219/1‑247 (MQ=255)                                                                     ggAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGc                                                                                                                                                                          >  1:151206/1‑250 (MQ=255)                                                                       aaaaaTGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGcaca                                                                                                                                                                       <  1:244389/251‑1 (MQ=255)                                                                             gCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCAtct                                                                                                                                                                 <  2:197099/251‑1 (MQ=255)                                                                                   tatCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTc                                                                                                                                                           >  2:248787/1‑251 (MQ=255)                                                                                    atCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTc                                                                                                                                                           >  2:101391/1‑250 (MQ=255)                                                                                                      aTCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTc                                                                                                                                        >  1:245903/1‑251 (MQ=255)                                                                                                          aTTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGAt                                                                                                                                    <  1:235737/251‑1 (MQ=255)                                                                                                                cccAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATcc                                                                                                                              >  2:52009/1‑250 (MQ=255)                                                                                                                  cAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCAcc                                                                                                                            >  2:8074/1‑251 (MQ=255)                                                                                                                        aTAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGACACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAAt                                                                                                                      >  1:80480/1‑251 (MQ=255)                                                                                                                                    cAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCt                                                                                                           >  1:152622/1‑250 (MQ=255)                                                                                                                                       tGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtatt                                                                                                        >  1:81610/1‑250 (MQ=255)                                                                                                                                                     cGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTAGCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTgg                                                                                         <  2:81610/251‑1 (MQ=255)                                                                                                                                                        cAAGCGTGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTc                                                                                       <  1:230272/250‑1 (MQ=255)                                                                                                                                                          aGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGACCTCTTCATCGTTATCGCCAAACAGCGACGGCAGCAACTGGTGTATCCCCTGGAGCACAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCcgc                                                                                    >  1:24637/1‑251 (MQ=255)                                                                                                                                                            cGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCgcgc                                                                                  >  1:209934/1‑251 (MQ=255)                                                                                                                                                                     ttGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGAt                                                                           <  2:194513/249‑1 (MQ=255)                                                                                                                                                                     ttGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCGTCTGTCAGGGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGGCCGCGCCGCCGATc                                                                          <  2:214841/250‑1 (MQ=255)                                                                                                                                                                                                              cATCGTTATCGACAAACAGCGACGGCAGAACCTCGTGTATCACCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGAt                                 >  1:235418/1‑250 (MQ=255)                                                                                                                                                                                                                           aaaCAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCt                   >  2:210694/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCAGCGACGGCAGAAACTGGTGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTc                  >  1:81335/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGGCAGAAACTGGTGTATCACCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCg          >  2:142819/1‑251 (MQ=255)                                                                                                                                                                                                                                            aCTGGAGTATCCCCTGTCGCAAAATGGTATGCGCCGTTTTCATAAATGAGAATAAATTGTCCTGTACCTCCAGTTGCACAAGCACATCATCTTCTGTCAGCGTCGCCTTTGGTCTTCCGATAGATCACCTCCAATGCAGGGCGTCTATTATATTACACGTTTGTTCCGCGCCGCCGATGCGTACTGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATTCCGTTCCCCAGCTCCCCGCCCGTCACTccc  >  2:226434/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      AATCCCAGGTCATGTCATCGGCAAA‑TCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGGGAAAAATGCGTGATATCGGCGTACAACCATTTATCCATTTTCCCCAACGCATAAATCAAACGCAGTGCAACATCAATATCGTCAAGCGGGCCGCTTTGGGCGAGTAACGGCTTCACTGCATATTCCACGATCTCTTCATCGTTATCGACAAACAGCGACGGCAGAAACTGGCGTATCCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCC  >  NC_000913/3074853‑3075337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑