Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,037	T→C	V77A (GTC→GCC)	fimH →	type 1 fimbriae D‑mannose specific adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,037	0	T	C	100.0%	43.6 / NA	14	V77A (GTC→GCC)	fimH	type 1 fimbriae D‑mannose specific adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

AATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTAT  >  NC_000913/4548807‑4549281                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaTGAAGCGATTTATTCCCCTGTTTCCTGTCCTTCTGATGGGCTGGTCGTTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCgg                                                                                                                                                                                                                                  <  1:134979/251‑1 (MQ=255) aaTGAAAGGATTTATTACCCTGTTTGCTGTACTGTTGATGGGCGGGTCGGTAATGGCCGTGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCgg                                                                                                                                                                                                                                  <  2:86022/251‑1 (MQ=255)   tgaaacccgTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTTGCTGAGCCCATGTTTATGAAAACCTTTCGCCCGTCGGGGATGTGTGGCAAAACCTTGCCGTGGATCTTTTCTCGCAAATATTTTGCCATAACTATTATCCTGGAACCATTTCAGACTATGCCACACTGCAACGAGACTccgcc                                                                                                                                                                                                                                >  2:165228/9‑247 (MQ=255)                 ccccTGTTTGCTGTACTGCTGATGGGCGGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGtt                                                                                                                                                                                                                  <  1:39918/250‑1 (MQ=255)                                                                       ggTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTAGCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTac                                                                                                                                                            <  1:55170/250‑1 (MQ=255)                                                                        gaaaaaCCGCCAATGGTACCGCTAGCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTacc                                                                                                                                                           <  2:159115/249‑1 (MQ=255)                                                                           aaaCCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTaccacc                                                                                                                                                        <  1:84409/251‑1 (MQ=255)                                                                                                                                                                                      aCGCAAATCTTTTGCCATAAAGATTATCCGGCAACCAATCAAGACTATGCCACCCCGCAACGAAGCTCGGCATATAGCGCCGTGCTACCTAATTTATCCGGGAACGTCAAATACAGTGGAAATAGCTATCCATTTACCACCACAAGCGGAACACCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGACTGTGAGAAGTGCGGGCGGGGTAGCGATTAAAGCTgg                                             >  1:119679/1‑251 (MQ=255)                                                                                                                                                                                              cTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGCAACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTCGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTa                                      >  1:39468/1‑250 (MQ=255)                                                                                                                                                                                                tttGCCATAACGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAAtt                                   >  2:76581/1‑251 (MQ=255)                                                                                                                                                                                                      aTAACGATTATCCGGAAAACATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTACTACCACCAGCGAAAAGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGGGGCGCTTTATTTGACGCCTGTGAGCAGGGCGGGCGGGGTGGCGGTTTAAGCTTGCCCATTAATTGCCGTg                             >  2:105033/1‑251 (MQ=255)                                                                                                                                                                                                         aCGATTATCCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCCGTGCtt                          >  1:189495/1‑251 (MQ=255)                                                                                                                                                                                                                     aaaCCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGGGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAg              <  1:227247/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGGTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGGGGCGCTTTTTTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGGTTAAAGCTGGGTCATTAATTGCCGGGCTTATTTTGCGAAAGACCAACAACtat  >  2:57372/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGCAAATCTTTTGCCATAACGATTATCCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACTAT  >  NC_000913/4548807‑4549281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑