Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,329,7430GT96.5% 94.8 / NA 29D261Y (GAT→TAT) dacBpeptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (14/14);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCA  >  NC_000913/3329515‑3329987
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
ggTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGTGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAt                                                                                                                                                                                                                                <  1:157979/251‑1 (MQ=255)
    atatGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATcccc                                                                                                                                                                                                                            >  2:183848/1‑248 (MQ=255)
        ggCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCGGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGGTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGa                                                                                                                                                                                                                        <  1:36130/251‑1 (MQ=255)
            tttATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCg                                                                                                                                                                                                                     <  1:179656/250‑1 (MQ=255)
                  cgcgTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCgggaaact                                                                                                                                                                                                              >  1:102301/1‑245 (MQ=255)
                              tattaCCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAAAACTGCTGCGCCAGAc                                                                                                                                                                                                  >  2:208424/1‑251 (MQ=255)
                                   ccccGTTACGATGTTCAGCCAGGGACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAgg                                                                                                                                                                                             <  2:199836/251‑1 (MQ=255)
                                             aTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCTCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAAcc                                                                                                                                                                                   <  1:120847/251‑1 (MQ=255)
                                                                               aggccgacgctaattACTGCGAACTGGATGTCGTGCCAGTCGACCTGATCCGCTTTCCGCTTACGAGATCCCGGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTACAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGAGTAGTTAAAACAGGCGGGTATCTCCTTGAGCGGCACGCAGCGGCGCCAGACTCAGATTAACGTACCGGGAGCGTGATTTGCCAGTTAACAGTCGGcccc                                                                                                                                                  <  2:111760/236‑1 (MQ=255)
                                                                                tGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACCGCTGCGCCCGACTCAGGGTAACGAACCTGGGACGGTAGTTGCCAGTAAACAGTCGGCCCCGc                                                                                                                                                >  1:91760/1‑251 (MQ=255)
                                                                                           aaTACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTg                                                                                                                                     <  1:125211/251‑1 (MQ=255)
                                                                                                        ggATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGGAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGc                                                                                                                        >  1:70198/1‑251 (MQ=255)
                                                                                                            gtggtgCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGaa                                                                                                                    <  1:62621/251‑1 (MQ=255)
                                                                                                              ggtgCAAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGaaa                                                                                                                   <  1:163886/250‑1 (MQ=255)
                                                                                                                 gCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATTACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGGACGGGAGTTGACAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTTAGATTATGCTGAAAAAgg                                                                                                               >  2:245305/1‑250 (MQ=255)
                                                                                                                   cAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACCGCTGCCCCCGACTCAGGTTAACGAACCTTGAACGGTAATTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGcgtaaaaaatcg                                                                                                             >  2:180132/1‑240 (MQ=255)
                                                                                                                                ccGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCg                                                                                                <  1:47741/251‑1 (MQ=255)
                                                                                                                                        cGCTGACGGGTGGCCTGACACAACGTTCTGAGCCGCTACCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCGGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACg                                                                                        <  2:177227/251‑1 (MQ=255)
                                                                                                                                              cGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTTTGCTGAAAAAGTCGGACAACATGATCGCCGATACGGttttt                                                                                  >  1:153460/1‑250 (MQ=255)
                                                                                                                                                  aTGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGGTAACGCAACTGGGACGGGAGTTGCCCATACACACTCGGCCCCGCCGCCCGATATGCTTAAGGTTATGTTGAAAAAGTCGGGCAACATGCTCGCCGATACGGTCTTCCGcc                                                                              >  2:76042/1‑250 (MQ=255)
                                                                                                                                                          cacaACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCCGGTTAACGAAACTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCCGCACGCACTGCTTAAGATTATGCTGAAAAAGACGGACAAAATGATCGCCGCTACGGTTTTTCGCATGATAGGc                                                                      >  2:164165/1‑251 (MQ=255)
                                                                                                                                                               cGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATgc                                                                 <  2:144594/251‑1 (MQ=255)
                                                                                                                                                                         cGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACATTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATTATCGCCGGTACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAt                                                       <  1:229156/251‑1 (MQ=255)
                                                                                                                                                                                                            aGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAAAACTGCTGCGCCAGACTCAGGGTAACGAACCTGGAAAGGGAGGTGCCCGTAAACAGTCGGGCCCGCTGCACGATCTGCTTAAGATTTTGCTGAAAAAATCGGACAACATGATCGCCGATACGGTTTTCCGCCTGATAGGCCCTTCGCGCCTCAATGTGCCTTGAACATGGCGGGCCGGGTGGGGCGcccc                    >  2:55261/1‑249 (MQ=255)
                                                                                                                                                                                                                     ggTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGaaa                                                                                                                   <  2:50277/147‑1 (MQ=255)
                                                                                                                                                                                                                     ggTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAAAGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGaaa                                                                                                                   >  1:50277/1‑147 (MQ=255)
                                                                                                                                                                                                                          aaTTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCCGGGCGGACGCCGTGCGTCAGATCCttc      >  1:210002/1‑249 (MQ=255)
                                                                                                                                                                                                                             tCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGCGCGGGACAATGCTGCGCCAAACTCAGGGTAACGAACCTGGAACGGGAGTTTCCAGTAAACCGTCGGCCCCGCCGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGGCAACATGAACGCCGGTACCGTTTTCCGCATGATAGGCCCTGCGCGCTTCAATGTTCCTGGAAACTGGCGGGCCGGGTCgggaggcggtcggtagatgctcgccc   >  2:41794/1‑227 (MQ=255)
                                                                                                                                                                                                                                gAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCa  <  1:208424/249‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
GGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTGCGCCA  >  NC_000913/3329515‑3329987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: