Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,705	C→T	Q275Q (CAG→CAA)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,705	0	C	T	100.0%	61.1 / NA	20	Q275Q (CAG→CAA)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

AATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCG‑TGGGTATGTACGGAGATAATGGCTT  >  NC_000913/82464‑82943                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aaTTGTCTAAATATTATTCACTTGCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTGTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGATTGTGCGAATGCGCCGCTGCCAACAATGGCTGTGTTTGCCGGGATCGGCATATTACAAATTTGGctaact                                                                                                                                                                                                                                         <  1:249659/251‑1 (MQ=255)   ttGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGATTCACCGCCGCAAGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGctaacta                                                                                                                                                                                                                                        >  1:31792/1‑250 (MQ=255)                   cACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCat                                                                                                                                                                                                                       >  1:216983/1‑251 (MQ=255)                        tctttATACCCAATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGCTCAGACCCATCGATTCTGGCGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATAtctc                                                                                                                                                                                                                  >  1:99411/1‑251 (MQ=255)                                          tCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCggg                                                                                                                                                                                                >  2:66685/1‑250 (MQ=255)                                              tGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGGTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTACTGGTGATTAATGGCGgtgggg                                                                                                                                                                                            >  1:167503/1‑248 (MQ=255)                                                     tttCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGgtgggggcggtgg                                                                                                                                                                                     >  2:147114/1‑241 (MQ=255)                                                              cgcACGCCCCGACCGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATTGCGGTGTGGACGTTGAGAATAT‑CCt                                                                                                                                                                           <  1:9026/251‑1 (MQ=255)                                                                               agGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGGGGACGTTTAGGATATTCTTTACGAAttttattgcc                                                                                                                                                           >  2:52769/1‑245 (MQ=255)                                                                                                                     tCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGGACAGTTTCCGGCACGCTc                                                                                                                    >  1:109563/1‑251 (MQ=255)                                                                                                                     tCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCt                                                                                                                     <  1:77920/250‑1 (MQ=255)                                                                                                                             cATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCCGGGAACAGTTTCCGGCACGCTCGCCGATcc                                                                                                            >  1:120059/1‑251 (MQ=255)                                                                                                                                                           cACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCAc                                                                              <  2:1666/251‑1 (MQ=255)                                                                                                                                                             cGCCATCCTGGTGTATACCGGAGGAGTTTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGTTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCTCCTTCCACCTGGCGTGCACCg                                                                            <  2:230742/251‑1 (MQ=255)                                                                                                                                                                                        gtgCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTcc                                                  <  2:55406/250‑1 (MQ=255)                                                                                                                                                                                                        gCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAAt                                 <  1:49260/251‑1 (MQ=255)                                                                                                                                                                                                                    tttgtttgCCGGGATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCCGGCCCAAATCGGCG‑TGGGTAt                    >  1:104191/1‑251 (MQ=255)                                                                                                                                                                                                                                gATCGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTTCGAACTTTGATTGCAATGATGACTTCTTCCAGGGAACAGTTTTCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGGGAGTTTACCACCGCCACGCCCCAATCGTCGGTGGGttgtcagggggta          >  2:90452/1‑238 (MQ=255)                                                                                                                                                                                                                                   cGGCATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCG‑TGGGTATGTACGGAGATAATg      <  1:66685/250‑1 (MQ=255)                                                                                                                                                                                                                                      cATATTACAAATTTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCG‑TGGGTATGTACGGAGATAATGGCtt  <  1:147114/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATAT‑CCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCG‑TGGGTATGTACGGAGATAATGGCTT  >  NC_000913/82464‑82943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑