Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,034,134	IS30 (–) +2 bp	coding (881‑882/1794 nt)	hyaB →	hydrogenase 1 large subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 271761	NA (NA)	27 (1.080)	24/490	0.2	100%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 1034135	0 (0.000)					coding (882/1794 nt)	hyaB	hydrogenase 1 large subunit
*	?	NC_000913	1034134 =	0 (0.000)	23 (0.930)	21/488	0.3	100%	coding (881/1794 nt)	hyaB	hydrogenase 1 large subunit
	?	NC_000913	1469296 =	NA (NA)					noncoding (1/1221 nt)	IS30	repeat region

GGGTTGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1034380‑1034134 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACAC  >  NC_000913/1469296‑1469538                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GGGTTGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGATTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTA                                                                                                                                                                                                                                                 <  2:127624/251‑1                                     gGACGCCCGACCTGATCGTTGGGATAGCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAAGCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACA                                                                                                                                                                                                             <  2:199330/250‑1                                      GACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACAT                                                                                                                                                                                                            <  2:239765/251‑1                                      GACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACAT                                                                                                                                                                                                            <  2:129077/251‑1                                            CGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGGTCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTT                                                                                                                                                                                                      <  2:179572/251‑1                                             gatctgcTCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACAAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTG                                                                                                                                                                                                     >  2:216021/8‑251                                              cCCTGATCGTTGGGATATCGATACCAGGCGTGGTCGCCAAACTCCTGCACCGGCTGCGGATCACCCAAATCCACTGGCAGCACATTGGTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGC                                                                                                                                                                                                    <  2:243973/250‑1                                                         GGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTT                                                                                                                                                                                         >  1:49363/1‑251                                                              ATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAACTCCACTGGCAGAACATTGTTGAAGTCGCCGTTAATCACCGAGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACAAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATG                                                                                                                                                                                    >  1:247294/1‑251                                                              ATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCATAAAGACCAGTGTAGATTCAAATGGTCAACGCAACAGTTATATGAAAACATGGGGTAGCGGAGGTCTTTTGAAT                                                                                                                                                                                     <  2:14725/250‑1                                                                                   CAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAG                                                                                                                                                               <  1:172734/251‑1                                                                                         CCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAA                                                                                                                                                          <  1:5817/250‑1                                                                                                 gGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGT                                                                                                                                                 <  2:58565/250‑1                                                                                                       GGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGA                                                                                                                                           <  2:203717/251‑1                                                                                                           CAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACT                                                                                                                                        <  1:134050/250‑1                                                                                                                                      TGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGCCTTTTCTCGCAAAAGACGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGAATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGCGACGCACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGA                                                                                                             >  1:123200/1‑250                                                                                                                                             GCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCG                                                                                                      >  2:10335/1‑250                                                                                                                                                                                                             GGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACC                                     >  1:250727/1‑251                                                                                                                                                                                                                    CGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATG                                >  1:68275/1‑249                                                                                                                                                                                                                            CTGAGAACGCATTTATCAGAAAGACCAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCG                      >  2:80081/1‑251                                                                                                                                                                                                                                   CGCATTTATCAGACAGACCAGTGTAGATTCAATTGGTCCACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGACCAGGCTTCAGTGACATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGT                >  2:101857/1‑250                                                                                                                                                                                                                                          ATCAGAAAGACCAGTGTAGATTAAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGAAGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAGCAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGTATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCAC         <  2:190609/250‑1                                                                                                                                                                                                                                                AAGACAAGTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACAC  >  1:198236/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GGGTTGTACCACGGGTCGGTGATGCCATCGAACGGATGACGCCCGACCTGATCGTTGGGATATCGATACCAGGCGTGGTCGACAAACTCCTGCACCTGCTGCGGATCAACCAAATCCACTGGCAGCACATTGTTGAAGTCGCCGTTAATCACCGCGCCGCCAGGCATCAGCAGACTTTTCTCGCCAAAGTCGTTGGCAATATCCGGGAATGCGCCGTAGCTGAGAACGCATTTATCAGAAAGACCAGtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1034380‑1034134 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACAC  >  NC_000913/1469296‑1469538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.