Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,641,276	C→T	P139S (CCG→TCG)	dtpB →	dipeptide/tripeptide:H(+) symporter DtpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,641,276	0	C	T	100.0%	94.1 / NA	27	P139S (CCG→TCG)	dtpB	dipeptide/tripeptide:H(+) symporter DtpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

TTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATCCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGAACCCGACTTCCGGCCAATGAGCTTCAGCA  >  NC_000913/3641036‑3641498                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                               tttGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCtt                                                                                                                                                                                                                        <  2:92673/249‑1 (MQ=255)           tGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTc                                                                                                                                                                                                            >  1:110885/1‑251 (MQ=255)                tATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGCTTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCCGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCt                                                                                                                                                                                                       >  1:243942/1‑251 (MQ=255)                   ggCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGtt                                                                                                                                                                                                    <  1:113335/251‑1 (MQ=255)                           ttCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGCCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATg                                                                                                                                                                                             <  1:189289/250‑1 (MQ=255)                                                            ggggACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGCTCAACATCGGCTCGTTGATAGCGTTATCGc                                                                                                                                                           >  2:16039/1‑251 (MQ=255)                                                             gggACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCt                                                                                                                                                          >  1:111726/1‑251 (MQ=255)                                                              ggACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTg                                                                                                                                                         <  1:47234/251‑1 (MQ=255)                                                                       gCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCtgtg                                                                                                                                                <  1:250862/251‑1 (MQ=255)                                                                              tGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCt                                                                                                                                          <  1:86968/250‑1 (MQ=255)                                                                                                   ggCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCCGTc                                                                                                                     >  1:120455/1‑250 (MQ=255)                                                                                                                        cGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTgcggggcgg                                                                                               >  1:134244/1‑251 (MQ=255)                                                                                                                                 gCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTACCCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCGTGATGGCGCATTCACTCTGTTCTATATGTCGATCAACATCGGCTCTTTGATAGCGTTATCGCTGGCCCCTGTGATCGCGGAGAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTa                                                                                      <  1:124483/251‑1 (MQ=255)                                                                                                                                   tACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAAttttt                                                                                    >  2:49494/1‑248 (MQ=255)                                                                                                                                                   gaTTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACa                                                                    <  1:84744/251‑1 (MQ=255)                                                                                                                                                   gaTTTTCAGCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTAc                                                                     <  2:220991/250‑1 (MQ=255)                                                                                                                                                         cATCGCCCTGGGGACTATCGCTTTCGGTAACGGCCTGTTTAAAGATAACCCAGCCAGCTTGCTGTCGAAGTGCTATCCGCAGAAAGATTCGCGGCTTGATGGCGCATTCCCCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCt                                                              <  2:65290/251‑1 (MQ=255)                                                                                                                                                                        tATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTg                                                >  1:72639/1‑250 (MQ=255)                                                                                                                                                                            gCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGa                                           <  2:102739/251‑1 (MQ=255)                                                                                                                                                                                   gTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGt                                    <  1:118499/251‑1 (MQ=255)                                                                                                                                                                                         gCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGaa                              <  1:46479/251‑1 (MQ=255)                                                                                                                                                                                         gCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGa                               >  1:100360/1‑250 (MQ=255)                                                                                                                                                                                         gCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGa                               >  1:210094/1‑250 (MQ=255)                                                                                                                                                                                         gCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGa                               >  1:103317/1‑250 (MQ=255)                                                                                                                                                                                                           cagccagcTTGCTTTCGAAGTGCTAACCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGGTAATTATCGCATTACTGGTTTACATCGCCTGTCGGGGAATGGTGAAAGACATTGGTTCTGAACCCGACTTCCGGCCAAtt            >  2:243094/1‑250 (MQ=255)                                                                                                                                                                                                            agccagcTTGCTTTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGGGAAAGACATTGGTTCTGAACCCGACTTCCGGCCAAtt            >  2:43035/1‑249 (MQ=255)                                                                                                                                                                                                                      cttTCGAAGTGCTATCCGCCGAAAGATTCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCCTTACTGGGTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGAACCCGACTTCCCGCCAATGAGCTTCAGCa  >  1:186396/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                               TTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGAGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCTGATTTTCATCGCCCTGGGGACTATCGCTGTCGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCTATCCGCCGAAAGATCCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTATCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATTACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGAACCCGACTTCCGGCCAATGAGCTTCAGCA  >  NC_000913/3641036‑3641498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑