Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,947	G→T	L14F (TTG→TTT)	gcvR →	putative transcriptional regulator GcvR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,947	0	G	T	100.0%	99.0 / NA	28	L14F (TTG→TTT)	gcvR	putative transcriptional regulator GcvR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/13);  total (15/13)

ATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCC  >  NC_000913/2599707‑2600183                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             aTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATc                                                                                                                                                                                                                                    <  2:192038/251‑1 (MQ=255)   tACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTCTGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTACAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGc                                                                                                                                                                                                                                  >  2:221678/1‑251 (MQ=255)                                gcggcAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTGCTGATTGTTGGATGCATGTTTTTTTTTTGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATCACCAGTTTGACACTGTCATCGCACCATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTc                                                                                                                                                                                                      >  2:176956/5‑250 (MQ=255)                                  gACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCAGCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATg                                                                                                                                                                                                   <  2:78326/251‑1 (MQ=255)                                       tACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagt                                                                                                                                                                                              >  1:47125/1‑251 (MQ=255)                                            cccGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTg                                                                                                                                                                                         <  1:180251/251‑1 (MQ=255)                                                        ggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACAGTATCTGTTGATCACTGCGTTTGGTTCCTATCGCCCTGGAATTTTGATCACCATCACCCGTCATGTCAGTAGGTGCGGCTGTAatat                                                                                                                                                                             <  1:22868/251‑1 (MQ=255)                                                                   gtgCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACa                                                                                                                                                                     <  2:162774/248‑1 (MQ=255)                                                                        aaCAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTg                                                                                                                                                             >  2:201241/1‑251 (MQ=255)                                                                                       ggTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCATGGCGATGCTGGGagaa                                                                                                                                              <  2:13824/251‑1 (MQ=255)                                                                                              ttGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTAAGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGAGCGCCCTGGAATTGTGAACACCATCACCCGGCATGTCAGTAGTCGCGTCTGTAATATTGAAGACAGTCGCCTGGAGATGCTGGGACGAGAGttg                                                                                                                                        <  2:144205/250‑2 (MQ=255)                                                                                                                      gacagaACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGtt                                                                                                               >  1:236102/1‑251 (MQ=255)                                                                                                                       acagaACCGTTCGGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTTTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTc                                                                                                              <  1:130809/251‑1 (MQ=255)                                                                                                                                   tGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCAt                                                                                                  >  2:107783/1‑251 (MQ=255)                                                                                                                                     aTTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTa                                                                                                <  1:155841/251‑1 (MQ=255)                                                                                                                                                   gTTTTTTTTATGCTTTCCTTAAGAACAACTCTCCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCa                                                                                  <  1:189788/251‑1 (MQ=255)                                                                                                                                                              gCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGtt                                                                       >  1:45799/1‑251 (MQ=255)                                                                                                                                                                    cTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCaa                                                                                 >  1:192498/1‑235 (MQ=255)                                                                                                                                                                    cTTAAGAACAACTCACCCCGTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCaa                                                                                 <  2:192498/235‑1 (MQ=255)                                                                                                                                                                       aaGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGc                                                              >  2:242790/1‑251 (MQ=255)                                                                                                                                                                        aGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACCCTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTAAAAGGTGcc                                                             >  1:88127/1‑251 (MQ=255)                                                                                                                                                                             aacTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACt                                                        >  2:91286/1‑251 (MQ=255)                                                                                                                                                                                                     aGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGATACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAgcg                                >  1:2690/1‑251 (MQ=255)                                                                                                                                                                                                       tttGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAgcgc                               >  2:154720/1‑250 (MQ=255)                                                                                                                                                                                                           acacTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGCATTGTGAACACCATCACCCGTCATGTCTGTAGTTTCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGCGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGATTCAACGTTACCGTTTAAAGGTGCCTAACTGGTTCTGTTAATCTTGATGAATCGCacga                           <  1:193265/250‑1 (MQ=255)                                                                                                                                                                                                                gTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCCGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGgcgc                     >  1:74467/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGc   <  2:47125/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCCTGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGCTGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGcc  >  2:244139/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             ATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCC  >  NC_000913/2599707‑2600183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑