Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,207	+A	coding (1026/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,207	1	.	A	100.0%	73.3 / NA	22	G342G (GGT→GGA)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (13/9);  total (13/9)

TAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGT‑TACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGA  >  NC_000913/1301994‑1302406                                                                                                                                                                                                                       |                                                                                                                                                                                                        tAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTc                                                                                                                                                                    >  2:175551/1‑251 (MQ=255)           ccgtgcGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACTTTGATCCATACCTGGGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATg                                                                                                                                                          <  2:129021/246‑1 (MQ=255)                           aGAAGCTGAAAAAAGAGACCCCGGCCGAAGTTCACGTTAATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAg                                                                                                                                          <  1:27345/251‑1 (MQ=255)                               gCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaa                                                                                                                                       >  1:37927/1‑250 (MQ=255)                               actaaaaaaaaaGAACCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCATCCAGAATGGTTTGGCTGGAGCCAGGaaa                                                                                                                                      <  1:213108/247‑1 (MQ=255)                                                  gACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGa                                                                                                                    >  1:200146/1‑250 (MQ=255)                                                     gAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGaaaa                                                                                                                 >  1:118187/1‑250 (MQ=255)                                                          tCACGTTGATCCATACCTGTGCACTTACTATTCCGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCCCTGAAACTAGGTATGGACCGCGATATCATTGTTACTAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAActgc                                                                                                           >  1:156367/1‑251 (MQ=255)                                                               ttGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGACTGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGAGAAAAAACTgctggct                                                                                                      >  2:241724/1‑251 (MQ=255)                                                                     cATACCTTTGCACTTACTATACCAAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCg                                                                                                <  1:230983/251‑1 (MQ=255)                                                                     cATACCTGTGCACTTACTATTACGAAATTAAAAACCAGAAACCGCCATTCAACGATGTGCGTGTGAGTACCGCACTGAAACTAGGCATGGACAGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCg                                                                                                >  2:86790/1‑251 (MQ=255)                                                                          cTGTGCACTTACTAGTAAGAAATTAACAACCAGCAACAGCCATTCAACGCTGCGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGtt                                                                                            >  2:238887/1‑250 (MQ=255)                                                                            gtgCACTTACTATTACGAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTtat                                                                                          >  2:211890/1‑250 (MQ=255)                                                                                            aaaataaaaaacCAGAAACCTCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGtt                                                                         <  2:35426/242‑1 (MQ=255)                                                                                               aTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGAc                                                                      >  1:210251/1‑251 (MQ=255)                                                                                                     aacCAGAAACCGCCATTCAACGAGGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACGCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCaa                                                                <  1:163089/251‑1 (MQ=255)                                                                                                                      cAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCt                                               >  2:2400/1‑251 (MQ=255)                                                                                                                          gATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGa                                           <  2:186578/251‑1 (MQ=255)                                                                                                                                     gcgtACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTGTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCAGCAACCTGTTGTATAACACCTCCGATCGGCATaaa                                 <  2:30440/250‑1 (MQ=255)                                                                                                                                     gcgtACCGCACTGAAACTAGGTATGGACCGCGATATCAATGTTAATAAAGTGAACGCGCAGGGCAACATGCCCGCCTA‑GGTATCCACTCCAACGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGTGCCAGGCAAAACGTAACGACGACACGACAAAACTGCTGGCTGAAGCGGGTTCTACCGCAGACAAACCGTCGACCATCAACCTGTTGTATAACACCGCAGATCTGCAtcaa                                 >  1:151245/1‑247 (MQ=255)                                                                                                                                                                  gcgATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTgg   <  2:59910/251‑1 (MQ=255)                                                                                                                                                                      tatCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGga  >  1:207860/1‑248 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                        TAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGT‑TACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGA  >  NC_000913/1301994‑1302406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑