Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	30.4 / NA	11	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/4);  total (7/4)

TTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATG  >  NC_000913/1652095‑1652567                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             tttCTGTTTATACTGGAGTTCGCCCTGGTGAGATGTGTGCACTTGGATGGGCGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCATCGGGTCATTCATCTTATTAAGCCAACAATCGACGCCCTCCGGAGCTAGATGACATTAAAGAGAATGAGCAAAGAGCATATCATTACTGTTCAACCCAGAGAGAATGGc                                                                                                                                                                                                                                >  1:17161/1‑251 (MQ=255)        ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCAGCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaaca                                                                                                                                                                                                                          <  1:163157/250‑1 (MQ=255)                                                                                 atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTGTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAc                                                                                                                                                <  2:237168/251‑1 (MQ=255)                                                                                                            cGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTTATTCATTTTATTAAGCCAGCAATCGACGCTCTCCGGCGTCAGATGACATTAAAGAGACTGAGCAAAAAGCATATCATTGATGTTTACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGGGACCATTTTACCGTTGACTCAATAAGGCAGATGTgggg                                                                                                                     >  1:211494/1‑250 (MQ=255)                                                                                                                                             aaTCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGAAGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGCGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATACGGCAGATGTGGGACGCAGCGATAAAACGTGACGGACTCCGCCATCg                                                                                    >  1:123004/1‑251 (MQ=255)                                                                                                                                                   gTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATc                                                                              >  1:248793/1‑251 (MQ=255)                                                                                                                                                         catCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGAGGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGTCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATc                                                                         <  2:206864/250‑1 (MQ=255)                                                                                                                                                                                              aGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAACATTTTACCGTTGACTCAATAAGGCAGATGTTGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCt                                   <  1:231709/251‑1 (MQ=255)                                                                                                                                                                                                             gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc                     >  2:94423/1‑250 (MQ=255)                                                                                                                                                                                                                    cAAAGGGCATATCATTCAAGCTCACCTCAGAGCGCATGGCAGAACAGAAAAACAAAAATGCGCCTTTGTTTTTCAACCTGGAGGGTCAGCGAGAGAAAAAACTTATGGTGACGATTTTACCGTTGACTCAATAAGGCAGATTTGTCACGCAGCGATAAAACGTGCCGGACTCCGCCATCCAAATTCATATCAGCCGAGACATACTTATGCCTGCTGGTCGCTGAAAGCAGGTGCTAACACGGGATTtat               >  2:166958/1‑249 (MQ=255)                                                                                                                                                                                                                               tCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATg  >  1:26606/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             TTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATG  >  NC_000913/1652095‑1652567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑