Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,510,905	G→A	G24G (GGC→GGT)	yhfX ←	putative PLP‑binding protein YhfX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,510,905	0	G	A	100.0%	82.0 / NA	24	G24G (GGC→GGT)	yhfX	putative PLP‑binding protein YhfX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/15);  total (9/15)

CACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAAT  >  NC_000913/3510680‑3511147                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cacaGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTgcgg                                                                                                                                                                                                                           >  1:94773/1‑251 (MQ=255)                                      ttGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCtt                                                                                                                                                                                       <  1:50953/250‑1 (MQ=255)                                         tAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTAc                                                                                                                                                                                   >  1:48068/1‑251 (MQ=255)                                                                gTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTc                                                                                                                                                             <  2:202062/250‑1 (MQ=255)                                                                       aaTGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCtt                                                                                                                                                     >  2:72702/1‑251 (MQ=255)                                                                          gCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGTCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTTCAAACATCTTGTTGCCTCGTTTCTGTTCCTTGaa                                                                                                                                                  <  1:235278/251‑1 (MQ=255)                                                                                      tCCGCCAGCCACGGATTACGACCAAACTGCTATGTCATCAGATACATTTCAATGCCGTAAAGCCGCGCCGTCTCAATCATCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTTCTGCCACAGGCTTAGTGCGGCGGAAATCAGCTCCGGGTTCTGCCGTTTCTATGCTTCTACAAACATCATGTTGCCTCGTTTCGGTTCCTGGAAGTAAATATTCa                                                                                                                                       <  2:235015/250‑1 (MQ=255)                                                                                        cGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAttt                                                                                                                                    <  1:244428/251‑1 (MQ=255)                                                                                         gccagccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCTACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAtttt                                                                                                                                   <  2:153720/251‑1 (MQ=255)                                                                                          cctgccaCTGATTACGACCACACTGCGTGGTTATTAGATACAGTGCAATGCCGTAAAGCCGCGCCGTCTCAATCATCCGCTTATCGTATTCCAGTATTGGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCAGAGGATTAGTGCTGCGGAAATCAGCGGCGGGGTCTGGCGTTTCAATGCTACTACAAACTTCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCagtt                                                                                                                                    <  2:234551/246‑4 (MQ=255)                                                                                                       ccGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAgg                                                                                                                     <  2:8382/250‑1 (MQ=255)                                                                                                           ccAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgt                                                                                                                 >  1:33431/1‑251 (MQ=255)                                                                                                            cAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCCCCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgtg                                                                                                                <  1:71026/251‑1 (MQ=255)                                                                                                            cAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAACCAGCGCCGTATCAATCAGCCGCTTACCCTTTCCCAGTATCTGATCAACGCCGATCACCCAGCTGCACGGGGCGATCTTACCCTGCTGCCACCGGCTTAGAGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgtg                                                                                                                >  1:11313/1‑251 (MQ=255)                                                                                                                      ggTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGa                                                                                                       <  1:109934/250‑1 (MQ=255)                                                                                                                                tACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGTGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGTATATTTAGGTTGTGCGACGTGGATTTGTCggg                                                                                              >  2:174263/1‑249 (MQ=255)                                                                                                                                  cAGTTCAATGCAGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCt                                                                                          <  1:249165/251‑1 (MQ=255)                                                                                                                                        aaTGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAAc                                                                                    <  2:15341/251‑1 (MQ=255)                                                                                                                                                          cGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGTCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCGGTTCCTTGAATTAAATAGTCATTTTTTTGAATATTGAGGTTGTGCGACGTGGATTGGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccat                                                                  <  1:153051/251‑1 (MQ=255)                                                                                                                                                                                               tCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCcttct                             >  1:129747/1‑251 (MQ=255)                                                                                                                                                                                                        aTCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACggg                    >  1:205038/1‑251 (MQ=255)                                                                                                                                                                                                         tCACCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGt                   >  1:3482/1‑251 (MQ=255)                                                                                                                                                                                                           aCCCAGCTGTCCGGGGCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTa                 <  1:99763/251‑1 (MQ=255)                                                                                                                                                                                                                          gCGATCTTACCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAAt  <  1:72702/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAAT  >  NC_000913/3510680‑3511147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑