Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,898	G→A	L113F (CTT→TTT)	yjeJ ←	protein YjeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,898	0	G	A	100.0%	50.8 / NA	15	L113F (CTT→TTT)	yjeJ	protein YjeJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/8);  total (7/8)

CAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAATCAGCAGGTCGCGCAGTTCCATGACAGACATAAAGAACAACGA  >  NC_000913/4373662‑4374135                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              cAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGAt                                                                                                                                                                                                                                 >  1:57714/1‑251 (MQ=255)             ccATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATGGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGt                                                                                                                                                                                                                      <  2:119800/250‑1 (MQ=255)                    ggTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCgt                                                                                                                                                                                                              <  2:204194/251‑1 (MQ=255)                                             gATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACCTTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTCTCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGc                                                                                                                                                                                      >  1:142153/1‑250 (MQ=255)                                                   cATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGGAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTa                                                                                                                                                                               <  1:244592/251‑1 (MQ=255)                                                                                     gAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTg                                                                                                                                             <  2:135976/251‑1 (MQ=255)                                                                                     gAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTg                                                                                                                                             <  2:201951/251‑1 (MQ=255)                                                                                                              aaTGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATcgcg                                                                                                                    >  1:186582/1‑251 (MQ=255)                                                                                                                   cAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCtt                                                                                                               >  1:105617/1‑251 (MQ=255)                                                                                                                             aTTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTCGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTg                                                                                                     >  1:103541/1‑251 (MQ=255)                                                                                                                                                 atgaatgaTTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCa                                                                                 <  1:58422/251‑1 (MQ=255)                                                                                                                                                                                      tCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAAt                                            >  1:88793/1‑251 (MQ=255)                                                                                                                                                                                       cATTTACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGCTTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAATc                                           >  1:139511/1‑251 (MQ=255)                                                                                                                                                                                                                  cGTCATGTAGCGTTAATACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAATCAGCAGGTCGCGCAGTTCCATgacagac                <  2:181824/251‑1 (MQ=255)                                                                                                                                                                                                                                aaTACGAAGGTAAAATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAATCAGCAGGTCGCGCAGTTCCATGACAGACATAAAGAACAACGa  <  1:177826/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              CAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTGAGGTAATACGTAATGCCAGTTCACGCATTTCCGCATTATTAATTGCATGAATGATTGCTCGCGCCAGCATTTCAATCTGCAACTCATTGACGACTAACTCACATTTGCTACCGTCATGTAGCGTTAATACGAAGGTAAGATTTTCGCCTTGATTATCGGTAAGTTCCAGCGTGTTAACCCGACGATTAATATCGGCATTTTTAAGCTCATCAACCAGAATTTCTGGGATGTTTTCTGCCATTTTCTTAATGACTTTATCGCGAGCTTGCTCATACTGAAGGTGTGCAGCAGCATCCAGTTTATGCTTTTGATGCAGACGACTTTCCAGCGCAATCAGCAGGTCGCGCAGTTCCATGACAGACATAAAGAACAACGA  >  NC_000913/4373662‑4374135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑