Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 229,552 C→T S129F (TCC→TTC)  dkgB → methylglyoxal reductase DkgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913229,5520CT93.8% 37.8 / ‑3.5 17S129F (TCC→TTC) dkgBmethylglyoxal reductase DkgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/10);  minor base G (0/1);  total (5/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01

CAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCC  >  NC_000913/229315‑229774
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              
cAGAAAGTGGCGTGACACGTCATGAACTCTACATCTCCACTAAAATCTGGATTGAAAATTTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGACAGCCTGCACAAATTGCGTACCGACTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGCGATCGGTATTTTCAACTTCACGATc                                                                                                                                                                                                                   >  2:228900/1‑251 (MQ=255)
     aGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGtt                                                                                                                                                                                                              <  1:119166/251‑1 (MQ=255)
            tGCCACGTCATGAACTCTACATCCCCACTAAAATCTGGATTGACAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAATAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGTCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGa                                                                                                                                                                                                        >  1:192519/1‑250 (MQ=255)
            tGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTg                                                                                                                                                                                                             >  1:16636/1‑245 (MQ=255)
            tGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTg                                                                                                                                                                                                             <  2:16636/245‑1 (MQ=255)
                                    ccacTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCACGTCTGCAAGAGAGCCTGCAAAAATTGCGTACCGATTATGCTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCt                                                                                                                                                                               >  1:140973/1‑251 (MQ=255)
                                     cacTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTg                                                                                                                                                                              <  2:10449/251‑1 (MQ=255)
                                                             cAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGAt                                                                                                                                                        <  2:198996/249‑1 (MQ=255)
                                                                                              gtgcctgcaaaaatggatggccGATTATGTTGATCTGACGCTAATCCACTGGCCGGCCCCAAACGATGAAGTCTCTGTTGAAGAGTTTATTCAGGCGCTGCTGGAAGCCAAAAAACAATTGCTGACGCGTGAGATCGGTATTAGCAACTTCACGATCCCGTTGATGGGAAAAGGGATTGCTGGTGTTTGGGCTGAAAAGATCGCTACGAACTAGATTGAACTTTCGCCTTATCTGCAGATCCGAAAAGTgg                                                                                                                     <  2:247209/231‑1 (MQ=255)
                                                                                                     cAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTg                                                                                                              <  1:244879/251‑1 (MQ=255)
                                                                                                            tGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCTGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTaa                                                                                                        <  1:30667/250‑1 (MQ=255)
                                                                                                                                                    gTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGt                                                               <  1:203475/251‑1 (MQ=255)
                                                                                                                                                                 gAAGTCTCTGTTGAAGAGTTTAAGCAGGCGCTTATGGAAACCAAAAAACAAGTGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTAAAAACATCGCTACTAACCAGATTGAACGCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTAAGGTAAGGCCCt                                                  <  2:171905/251‑1 (MQ=255)
                                                                                                                                                                           ttGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATg                                          >  1:147933/1‑249 (MQ=255)
                                                                                                                                                                              aaGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGtt                                     <  2:151796/251‑1 (MQ=255)
                                                                                                                                                                                                       aGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAAcaca            <  2:94461/251‑1 (MQ=255)
                                                                                                                                                                                                                 cAAGGGCTGACGCGTGAGATCGGTATTTACAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTTCTTCCTATATGACGCTGGCGTATGGTAAGGCCATGAAAGATGAGGTTATTGCGCGTATCGCAGCTAAACACAATGCGACTcc  <  1:235266/251‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              
CAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCC  >  NC_000913/229315‑229774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑