Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,829,887 G→A A293A (GCC→GCT)  astA ← arginine N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,829,8870GA96.4% 78.4 / ‑5.2 30A293A (GCC→GCT) astAarginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (7/20);  total (10/20)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGG  >  NC_000913/1829641‑1830124
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
tgccttgccATAACCCCTCGCCCGATACCGGAGTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTGCTCTGCGCCCAGGCGCACCAGACGAACGCTATCCCCGGCTTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTGCATTAGCg                                                                                                                                                                                                                                              <  2:145254/250‑1 (MQ=255)
tgccttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCg                                                                                                                                                                                                                                              <  1:106037/250‑1 (MQ=255)
     ggccttatcgccTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTTGCCCTTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACTAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATGTTCATTAGAGACcagg                                                                                                                                                                                                                                        <  2:180639/241‑1 (MQ=255)
                                             gcgATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCAGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCa                                                                                                                                                                                               <  2:171897/251‑1 (MQ=255)
                                                      ccTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAGCACCCCGCGGAAATGGGGATAATTTTCATTAGCGACCCGGCAGGCTGGGAAATCGCCCTGCGCAGGATGc‑ccctttgcccactcccccc                                                                                                                                                                                      >  2:207802/1‑234 (MQ=255)
                                                       cTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTTGCGACCCGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCCTCCACTTccccc                                                                                                                                                                                       >  2:47477/1‑246 (MQ=255)
                                                                 aTCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAGCACCCCGCGGAAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCCGCCGACtttt                                                                                                                                                                            >  1:242524/1‑250 (MQ=255)
                                                                              tAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCCCGGTTGCCGGATCGGTACGCACCAGCCCCACGCGGAAATGGGGATAATTTTCCTTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCCGGCTGC‑CCTTCTGCCCCTTCCCCCACCCCACTTTTACGGAATgcgcgcc                                                                                                                                                              >  2:75/1‑250 (MQ=255)
                                                                                                   tttCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCa                                                                                                                                         <  2:240730/250‑1 (MQ=255)
                                                                                                        cctcTGCGCACAGGCGCTCCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGc                                                                                                                                      <  2:9726/248‑1 (MQ=255)
                                                                                                                    ggCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCa                                                                                                                       <  1:236618/251‑1 (MQ=255)
                                                                                                                              actaaCGCGAGCCCCGGCGAGGCATTTGAGGGCATCCAGTTGTGCGGCGGATCAAAAT‑AAACGCCCGGTTGCCGGGTCTGTAAGCACCAGGAACCCGCGGAAATGGCGATAATTTTCATTAGCGACCCGGCACGCTGGGAAATCGTCCTGTGTAGGC‑GCACCTTCTGCCAATTCCACGCCCCCACTTTTACAGATTGCG‑ACACCGCCGTCGCCGTCACACTCACGCGTCCGCCCCCCGTCACAGAtttctc                                                                                                             >  2:34961/4‑246 (MQ=255)
                                                                                                                                       aTCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTAc                                                                                                    <  1:223134/251‑1 (MQ=255)
                                                                                                                                                 gggCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaa                                                                                          <  2:50958/250‑1 (MQ=255)
                                                                                                                                                         gAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttctt                                                                                  <  1:161014/251‑1 (MQ=255)
                                                                                                                                                            ggCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGTGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGTGCAC‑GCGGTCGATGTCACACTCCAGCGTCGGCCCACCCTCAAAGCTGTCGATATAGTTAAGGGAGCGAAAACCttcttttt                                                                               >  1:130705/1‑249 (MQ=255)
                                                                                                                                                                      tgtgCGGCGG‑TTAAAATCAAACGCTCGGTTTCCGGGTCGGTACGCACCAGCCCCCCGCGGAAATGGTGATAATTTTCATTCGCGACCCGGCAGGCTGGGAAATCCCCCTTCCCCCGCTTC‑CCTTCTGCCCCTTCCCCCAGCCGACTTTTACGGATTGCGCGCAC‑CCCGTCGGTTTCACCCTCCAGCGTCGTCCCCCCGTCAAAGATGTCGATATAGTTTCGGTAGCGCAAACCTTCTTTCTCCAGcccccc                                                                     >  1:107473/1‑246 (MQ=255)
                                                                                                                                                                                     aaTCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCgg                                                       <  1:205701/251‑1 (MQ=255)
                                                                                                                                                                                                     gCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTg                                       <  1:231526/251‑1 (MQ=255)
                                                                                                                                                                                                      ccGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGa                                      <  2:159780/251‑1 (MQ=255)
                                                                                                                                                                                                           tCGGTACGCACCAGCCCCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGCTGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGAGAAAACCTTCTTTCTCCAACACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTgcacgat                                 >  2:209200/1‑245 (MQ=255)
                                                                                                                                                                                                           tCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGAt                                 >  1:174974/1‑251 (MQ=255)
                                                                                                                                                                                                              gTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCGCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGAc                              <  2:242524/251‑1 (MQ=255)
                                                                                                                                                                                                              gTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGAc                              <  1:99082/251‑1 (MQ=255)
                                                                                                                                                                                                                         caccacGCGTAAATGGTGATAATTTTCATGAGCGACCAGGCAGGCTGGGAAAGCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTGTGCGGATGTACCTGACCGATGACGTCCTGGGCtt                   <  2:126138/251‑1 (MQ=255)
                                                                                                                                                                                                                         caccacGCGGAAATGGTGATAATTTTCATCAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCtt                   <  1:28485/251‑1 (MQ=255)
                                                                                                                                                                                                                              cgcgGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGGACCTGACCGATGACGGCCTGGGCTTCCTgg              <  2:79770/251‑1 (MQ=255)
                                                                                                                                                                                                                                          gATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTTCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg  <  2:127136/251‑1 (MQ=255)
                                                                                                                                                                                                                                                tttCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg               >  2:43852/1‑232 (MQ=255)
                                                                                                                                                                                                                                                tttCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg               <  1:43852/232‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
TGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGG‑TTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGC‑CCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCAC‑GCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGG  >  NC_000913/1829641‑1830124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑