Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	920,904	C→T	I186I (ATC→ATT)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	920,904	0	C	T	100.0%	65.8 / NA	22	I186I (ATC→ATT)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

GTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGG  >  NC_000913/920665‑921142                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                gTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGAGATTCTGCATCAGcc                                                                                                                                                                                                                                      >  2:178231/1‑250 (MQ=255)    gAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCTGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTgcg                                                                                                                                                                                                                                   <  1:149509/251‑1 (MQ=255)                 tCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGCCAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTg                                                                                                                                                                                                                                     >  2:211213/1‑236 (MQ=255)                 tCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTg                                                                                                                                                                                                                                     <  1:211213/236‑1 (MQ=255)                    aTGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACg                                                                                                                                                                                                                   >  2:155011/1‑251 (MQ=255)                                           cGACTGCTTAGTGCCCAGGAGATGCTGCAACGGCTGGGGCTGGCAGCCCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCACCCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCACCAGCGCGATCGTGGGCATACGTTGATGATCGTCAcacccgagcc                                                                                                                                                                                            >  2:89506/1‑242 (MQ=255)                                                                              ggggctggAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAGGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGaa                                                                                                                                                          >  1:99500/1‑250 (MQ=255)                                                                                               cAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGAACCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAAtt                                                                                                                                        >  2:92522/1‑251 (MQ=255)                                                                                                              cacaGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTccc                                                                                                                         <  1:66425/251‑1 (MQ=255)                                                                                                                        gggTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGaa                                                                                                                >  2:217994/1‑250 (MQ=255)                                                                                                                              tCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGACCGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTg                                                                                                          >  1:222150/1‑250 (MQ=255)                                                                                                                                    aCAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGtt                                                                                                    >  2:1888/1‑250 (MQ=255)                                                                                                                                            cacgcagcgcgggggcatgattgaAGGGTGGTAAGGTATTTCTTGCCGA‑GGACCAACCGGGCGCCCGGGACACCCATTCGGGCGAAGGGGTGATGGCGTTTCTACATCAGCGGCGCGGTGGTGGGCATACGGTGATTATCGCCCCCCCCGATCCGCAGGTCGCTGCTCGGGCCGAGCGGGTGATCGAAATTCGCGCGGGCGAATTTGGGCGCAATCCCCCCGCCATTGAAAAAGTGAATGTTACTGGCggg                                                                                           <  2:173101/230‑1 (MQ=255)                                                                                                                                             cAGCTTCGCGCGGGCATTGATGAACGGTGGTCATGAAATTCTTGCCGGTAAACCACCCGG‑CGCCCGGGACAGCCATTCTGGCAAAGAGGTGATGGCGATTCTGCATCAGCGGCGGGATCGTGGGCATACGGTGATTACCGTCCCCCACGATCCGCAGGTCGCTGCGCAGGCGGGGGGGGTGATCAAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGa                                                                                          <  1:235740/251‑1 (MQ=255)                                                                                                                                              aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGAc                                                                                         >  1:174852/1‑251 (MQ=255)                                                                                                                                               gcatggggcgGGCATTGATGAACGGGGGTCGGGTAATTCTTGCCGATGAACCACCCGG‑CGCACTGGACAGCAATTCTGGGGAAGGGGGGATGGCGTTTCTGCATCAGCGGCGCGATGGGGGGCTGAGGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGGGGGTGATCGAAATTCGCGAGGGCGAAATTGGGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACg                                                                                        <  2:148552/244‑1 (MQ=255)                                                                                                                                                          gCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCtgttgt                                                                             >  1:161369/1‑251 (MQ=255)                                                                                                                                                                atgaGCGGTGGCTAGGAAATTCTTGCAGATGAACCACCCGG‑CGCACTGGACAGCCTTTCTGGCGAAGAGGTGATGGCGATTCTGCATCACCGGCGCGATCGTGGGCAGCCGGAGATTATCGTCCCCCGCGATCCGCAGGTCTCTTCTCGGGCCGATCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCCCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAacac                                                                       <  1:162944/251‑1 (MQ=255)                                                                                                                                                                                     ttggCCGGTGACCCACCCGG‑CGCCCTGGACAGCCATTCGGGCGAAGAGGTGATGCCTTTTCTGCATCAGCGGCGCGTGCGGGGGCATGCGGTGATTATCGTCCCCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGGGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑Gt                                                  <  2:34496/247‑1 (MQ=255)                                                                                                                                                                                                    accGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGGCGGAAACTGTTGTCAAAACCGGGTCTGGGTTGCGGCATGTTTG‑CAGCGGttttt                                   >  2:106523/1‑249 (MQ=255)                                                                                                                                                                                                                 gACAGCCATTCTGGCGAAGAGGTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGCAACTGTTGTCAACACCGTGCCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTTACGAGGCGCTGAc                      >  2:117109/1‑251 (MQ=255)                                                                                                                                                                                                                                     ggTGATGGCGATTCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGGGTCTGGCTGGCGGCA‑GGTTGTCAGCGGTTTTTACGAGGGGCTGACGATGGCCTggcggggggcgg  >  2:182293/1‑245 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                GTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGG‑CGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCAGGCCGAGCGGGTGATCGAAATTCGCGACGGCGAAATTGTGCGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCA‑GTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGCTGG  >  NC_000913/920665‑921142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑