| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,465,154 | C→T | L25F (CTC→TTC) | gspM → | Type II secretion system protein GspM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,465,154 | 0 | C | T | 92.3% | 27.0 / ‑6.1 | 13 | L25F (CTC→TTC) | gspM | Type II secretion system protein GspM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/7); minor base A (1/0); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
GGAGGCGAACTATAGCCAGAAGCAAAAGTCGCTGACCTTAAAAATGAGTGCTAAGAGTGAAGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGG‑AAGCAAAACGTGAGAATTTGGCTATCA > NC_000913/3464917‑3465358 | ggAGGCGAACTATAGCCAGAAGCAAAAGTCGCTGACCTTAAAAATGAGTGCTAAGAGTGAAGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGtttttt < 2:93703/250‑1 (MQ=255) cAGAAGCAAAAGTCGCTGACCTTAAAAATGAGTGCTAAGAGTGAAGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGCTCTCTCGTTCGGCGTTTTTTTTT‑TGCTTgggggggt > 1:205025/1‑246 (MQ=255) aaGAGTGAAGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGCTCTCTCGTTCTGGGTTTTTTGTT‑GGGTTGGGGGGATAAAAACGAAAGATACCTACATTGCAGAACATCAggcc > 1:100912/1‑248 (MQ=255) aaGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACgcgc < 1:144212/251‑1 (MQ=255) atcgTTTCTTTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTaaga < 2:117267/251‑1 (MQ=255) tAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGTTCGTTGCGGCACTGGCGGATCTCTCCATCCGCGTTTTTTTTTTGGTTTGGGGGGTTAAAACC‑ATAAATCACTACCTTGCACGGAATTAGTTCCCCCCGC‑A‑AAAAATTAAAAAGGaaattcaaa > 2:170915/1‑240 (MQ=255) aaaaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCTTCGGCGGTTTTTTTT‑TGCTTGGGGGGGTAAAACCCATAGATACCTACCTTTCCGAGCATCCGGCCCCCCCGC‑ACAAAATTTAAAAGGGACTCCAAATG‑AAGCAGGATCCAGCGAGCcccccaggt > 1:44339/1‑246 (MQ=255) aaaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGGGTTTTTTTTT‑TGGTTGGGGGGGTAAAAACGACAGATAACTCCATTGCAGAGCATCAGTCCCACGCGCCA‑AAAAATAAAAAAGG‑CATCAAAAGGATGCAGGATCAAGCGAGAACCCCTgggt > 1:87323/1‑249 (MQ=255) aGGAACTCATGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAat < 1:221796/251‑1 (MQ=255) aTGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGG‑AAGCAAAACGTg < 1:235192/251‑1 (MQ=255) aaaTCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGTATGCAGGAt < 2:199414/156‑1 (MQ=255) aTCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGTTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGGCATCCTGCGCTTACGCAGCCCATCAAAAAGAGCCTTCTCGAGG‑AAGCAAAACGTGAGAATTTGGCTATCa < 2:152633/251‑1 (MQ=255) tCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGGTCTCTCCTTCGGCGTTTTTTTTT‑GGGTTTGGGGGGTAAAACCGATAGATACCTACATTGTAGAGCATCAGGCCCCCGCGC‑A‑AAAAAATAAGAAGGAAATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCCTCAAAAATATCCTTCTCGAGGGAAGAAAAAcagagaaatttgttatcac > 2:145440/1‑233 (MQ=255) | GGAGGCGAACTATAGCCAGAAGCAAAAGTCGCTGACCTTAAAAATGAGTGCTAAGAGTGAAGCTAATATCGATCGTTTCTGTGAGTTAACACAGTCCTGGCTTCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTT‑GGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGC‑A‑AAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGG‑AAGCAAAACGTGAGAATTTGGCTATCA > NC_000913/3464917‑3465358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |