Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,157	G→T	P10H (CCT→CAT)	yhjR ←	PF10945 family protein YhjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,157	0	G	T	100.0%	63.6 / NA	19	P10H (CCT→CAT)	yhjR	PF10945 family protein YhjR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/10);  total (9/10)

CCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAATTTCA  >  NC_000913/3695914‑3696399                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ccAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGt                                                                                                                                                                                                                                             <  1:62150/251‑1 (MQ=255)              gcggTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTg                                                                                                                                                                                                                                >  1:206373/1‑251 (MQ=255)                                             gCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATa                                                                                                                                                                                                  >  1:232299/1‑250 (MQ=255)                                                         aTCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTc                                                                                                                                                                                      >  1:74312/1‑250 (MQ=255)                                                                tACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAACCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGACGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGAc                                                                                                                                                                               >  2:160246/1‑250 (MQ=255)                                                                       cttCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATCTCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTa                                                                                                                                                                        >  2:29280/2‑250 (MQ=255)                                                                                           tgttgCGCAAACTCGGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGt                                                                                                                                                    <  1:17145/250‑1 (MQ=255)                                                                                                            cAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCg                                                                                                                                     >  1:27/1‑248 (MQ=255)                                                                                                                                               gTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACg                                                                                                <  1:3882/250‑1 (MQ=255)                                                                                                                                               gTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGAGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACg                                                                                                <  2:193441/250‑1 (MQ=255)                                                                                                                                                    cgTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACTGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAAc                                                                                            >  1:141691/1‑249 (MQ=255)                                                                                                                                                             atatCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTa                                                                                 <  1:25439/251‑1 (MQ=255)                                                                                                                                                                          aaaTATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGAc                                                                    <  2:210749/250‑1 (MQ=255)                                                                                                                                                                                cAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGTCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTaaaag                                                              <  2:202643/251‑2 (MQ=255)                                                                                                                                                                                 aGGCAGTGAAACTGCATGCTTTAACGCCACAATATCATTCTGGAAGATATAGACTCTCGCGGGATCATGCAGAGTATCTGATTCATTGTTATTCATTTTGCTTGATCAATATGAAATAACCGTACCGTTCATGCGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGCGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATc                                                             >  2:249720/1‑251 (MQ=255)                                                                                                                                                                                                                                 ataGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAg             <  1:48750/251‑1 (MQ=255)                                                                                                                                                                                                                                    gCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCCCCTGCTAAATCTGATAAGttt          >  1:247572/1‑251 (MQ=255)                                                                                                                                                                                                                                           gcgGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAATTTc   <  2:232299/251‑1 (MQ=255)                                                                                                                                                                                                                                            cgGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAATTTCa  <  1:271/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CCAGGCTAATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAATCTGATAAGTTTTAATTTCA  >  NC_000913/3695914‑3696399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑