Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,703,531	G→T	Y106* (TAC→TAA)	dppD ←	dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,703,531	0	G	T	100.0%	78.4 / NA	24	Y106* (TAC→TAA)	dppD	dipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/11);  total (13/11)

CACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTAC  >  NC_000913/3703291‑3703761                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       cACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTTTTGCCCCCCTGGTGCACCTTAATCGCTTCCATAAACTGGAAA‑ACCACGGGTTAGCACCGGt                                                                                                                                                                                                                              >  1:111999/1‑251 (MQ=255)                    ggTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCCGTTGCACCTTAATCTCCTCCATAATCTGGAAAACCCA‑GGTTTTGCCCGGGGTAAGGCTGGTCATCGGGTc                                                                                                                                                                                                           >  2:132903/1‑251 (MQ=255)                              cAATCAGCAGTTTTGGCCGACAGGCAATCGACATAGCGATCATCACGAGCTGGCTCATGCCGCCGGCAAGCTGATGCGGGCAAACATCCAGACGCGATGCCGGATCGGGAAAACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGAATTTTGTTTGCGCCCCGATGCCCGTTAATCCCTTCCATAAATTGGGAA‑CCCACGGGTTAACACGGGTTAAGGCTGGGCATTGGGGCCttggagat                                                                                                                                                                                                  >  1:24415/1‑243 (MQ=255)                                              ccGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGcac                                                                                                                                                                                 <  2:89980/251‑1 (MQ=255)                                                                ggcgtgcatcaCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGct                                                                                                                                                               <  2:201880/245‑1 (MQ=255)                                                                      catcaCGCGCTGGATCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTATGTTGCCGCCCTGAGGCACCTTAATCGCTTCCCTAATCTGGAAA‑CCCCCGGTTTAGCACGGGTTAAGGCTGGTCATCGGGGCCTGGAAGAACATCGCCACTTCGGCCCCCACCAGGTTGCGGCGCTCttttt                                                                                                                                                         >  1:170781/1‑251 (MQ=255)                                                                         caCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTGCCGCCCTGATGCACCTTAATCTCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGGAGATCATCGCCACTTCGGCACCCACCAGGGTGCGGCGCTCtttttttt                                                                                                                                                      >  1:87761/1‑248 (MQ=255)                                                                                                   gCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTGTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTa                                                                                                                            <  2:241480/251‑1 (MQ=255)                                                                                                         gCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTcc                                                                                                                      <  1:71926/251‑1 (MQ=255)                                                                                                                  cATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTTCCGCCCTGATGCACCTTAATCGCTTCCATAATATGGAAA‑CCCACGGTTTAGCACGGGGTAAGGCTGGGCATCGGGTCCTGGGAGATCATCGCCACTTTGGCACCCCCCCGGGTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCt                                                                                                             >  1:194070/1‑251 (MQ=255)                                                                                                                     ccAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGcc                                                                                                          <  2:94349/251‑1 (MQ=255)                                                                                                                     ccAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGcc                                                                                                          <  2:71381/251‑1 (MQ=255)                                                                                                                                         ggAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGGTTAGCACGGGGTAAGGCTGGTCATCGGGGCCTGGAAGATCATCGCCACTTCCGCACCCACCAGGTTGCGGCGCTCTTTTTCTTAGATACGCTGCAAATCCTGGCCGGTAAAATCCCGTTTTTTTTCCCTTACGCGGCCCGGATaatc                                                                                      >  1:223416/1‑251 (MQ=255)                                                                                                                                               ccGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATGACGCGGCCCGGATAATCAATCAg                                                                                <  2:167460/251‑1 (MQ=255)                                                                                                                                                       aTTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCACCCGGAAGCACCTTAATCGCTTTCACAATCTTGAAA‑CCCACGGTTTAGCACCGGGTAAGGCTGGTCATCGCGTACTGGAAGATAATCCGCAATTCAGCACCCACCCGGATGAGGACCTCTTTTTTAGAGATACCATGCAAATCCTGGCCGTTAAAATCCAGATTTTGTGCCATTATGAGGCCCGGCAAATAAATCAGcacactat                                                                        >  1:146789/1‑244 (MQ=255)                                                                                                                                                            gcagGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGcc                                                                   <  2:4750/251‑1 (MQ=255)                                                                                                                                                              agGTCAATCGCTCGCTGACGTCGGGTACTTTTGTTGCCGCCCTGAGGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTGGCGGCGCTCTTTGTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCACTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAg                                                                 <  2:231319/251‑1 (MQ=255)                                                                                                                                                               ggTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCTCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACTGTTTAGCACGGGGTAAGGCTGGTCATCGTGTCCGGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCTCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTGTTTCTGCCATTTCGCGTCCCGGATAATCAATCAGCCCCATAATCGCCAGt                                                                <  1:161505/251‑1 (MQ=255)                                                                                                                                                                gTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGTCGACCTGTTCCCCACTAATAGCTTCCCTAATATCGCAA‑CCCACGGTTTAGCCCGGGGTAAGAGTGGCCACCGGGTCCAGGAAGAACATTGCCACTTCGGCACCCCCCCGGTTGCGGGGACCTTTTTTTGAGGGACCCTGCAAATACTGGCCGTTAAACTCCAGGTTTTTTGACATTAATCGGTACGGAGaatcaatccggccaataaacgacagtg                                                               >  1:240837/1‑232 (MQ=255)                                                                                                                                                                       gctcgctGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAAACGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGGCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCCGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGAc                                                        >  1:72342/1‑251 (MQ=255)                                                                                                                                                                                                     cccTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCCCCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAAt                          >  1:160680/1‑251 (MQ=255)                                                                                                                                                                                                          aTGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTAAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATcccc                      >  2:93063/1‑249 (MQ=255)                                                                                                                                                                                                              aCCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCAc                 <  1:57123/251‑1 (MQ=255)                                                                                                                                                                                                                             aTAATCTGGAAA‑CCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTAc  >  1:97821/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       CACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAA‑CCCACGGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTAC  >  NC_000913/3703291‑3703761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑