Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,294	C→G	noncoding (35/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,294	0	C	G	100.0%	34.6 / NA	13	noncoding (35/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/6);  total (7/6)

AGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287061‑1287498                                                                                                                                                                                                                                          |                                                                                                                                                                                                             aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGCGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGCAAGGGATGGTGGGGGGGGAAGGATTCGAACCTTCGTAGTCGATGACGGCAGATTTAGAGTCTTCTCCCttttgc                                                                                                                                                                                             >  2:128437/1‑248 (MQ=18)        gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGACGCTTCTCATCCTTCCCCGCATGGGCAGCATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAATGTGCTGGTGGTGGGGGAAGGATTCGAACCTTCGCAGTCGATGACGGCAGTTTTAGAGTCTGCTCCCTTTGGCCGCGCgg                                                                                                                                                                                      >  2:144495/1‑251 (MQ=18)           gggggggAAGGATTACTCAGCGCTGCGCTCTTCGCCCGTCGGGTCGTTGCCTGGGGCAACGCTCTGTCGCTGTCGCTCGAGTCGAACCTTGTTCGAAGCTTCTCATCGTTCCCCGCGTGGTCAGAATATTTAATTGCGGACTCGCTGGCGAGCTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCGCGGGaa                                                                                                                                                                                   <  2:33307/249‑1 (MQ=18)                         aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCGAAGCTTCACATCCGTCCACGCATGGGCAGAATATTTAATTGCGGAGGCGTTGGAAAGTTCGGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGt                                                                                                                                                                      <  2:33049/250‑1 (MQ=21)                                 cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGGAATTCaact                                                                                                                                                             >  1:66944/1‑249 (MQ=255)                                           cGCCCGTCGGGGCGCTGCGTGCGTCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGTATGGGCAGAATATTTAATTGCGGATGCGTTTGGAAGTTCAGTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTaa                                                                                                                                                   <  2:241191/251‑1 (MQ=25)                                                     gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGAt                                                                                                                                         <  1:101348/251‑1 (MQ=255)                                                                   cAACGCTCTCGCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGGAGTTCAGGGACTTATGAAAGTTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAg                                                                                                                           <  2:12825/251‑1 (MQ=255)                                                                                 tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGtcgct                                                                                                             >  1:80061/1‑251 (MQ=255)                                                                                                                                  ggCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAACGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGGAATTCATTTTTTGAGGTAATGCTTGAGATGGTGGTGGGGGACGGATTATTCGACATTTCGCTCCTCACCCTTGGGGTCGTTGCCTGTGACAAGGTTCTCTCCCttt                                                            >  2:196010/1‑251 (MQ=25)                                                                                                                                    cAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGAAATTCATATTTTGAGGCATTCCTTGATATGGTGGGGGGGGAAGGATTATTCGTCTCTTCGCTCCTCACCCTGCGGGCCGTTGGCTGTGGCAACGTTCTTTCCCTTTct                                                          >  1:186848/1‑250 (MQ=25)                                                                                                                                                               gAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTtc                               <  1:228796/251‑1 (MQ=255)                                                                                                                                                                                            ggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGGAATTCAAATTTTGAGGTAATGCTTGAGATGGGGGAGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGGTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGGGTGAAAACTTTc  >  1:141584/1‑251 (MQ=38)                                                                                                                                                                                                                                          |                                                                                                                                                                                                             AGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTC  >  NC_000913/1287061‑1287498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑