Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 549,527 G→T G391W (GGG→TGG)  ylbE → DUF1116 domain‑containing protein YlbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913549,5270GT96.2% 76.6 / NA 26G391W (GGG→TGG) ylbEDUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (14/11);  minor base A (1/0);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGT  >  NC_000913/549287‑549770
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    
ggAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCTGGCAGATTgg                                                                                                                                                                                                                                            >  1:101338/1‑251 (MQ=255)
                           ggCGTAACGCGCTTTGGCGGTGCGGGCGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCTGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATGTTCAGGGCGCATGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTgg                                                                                                                                                                                                                  <  2:169036/250‑1 (MQ=255)
                                     gcTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCTTGCCGATTGGCGCAGGCACCGTGCGGGGACCCCCGGCG‑GGCtttta                                                                                                                                                                                                       >  1:123357/1‑248 (MQ=255)
                                            cGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGCAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCAGGCCGATGTGCCCGGGCACCGCGCGGGCAccccctgggtgctctgaactggcgg                                                                                                                                                                                                >  2:8532/1‑228 (MQ=255)
                                             ggTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCt                                                                                                                                                                                              <  2:37544/251‑1 (MQ=255)
                                                  gggTGGCATGGAAGCGGCAAGAGCGGGATCTGAAGAGATGGCGGAAAGTGACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGAACCGGGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAg                                                                                                                                                                                         <  2:89/251‑1 (MQ=255)
                                                         aTGGAAGCGGCAAGAGCGGTATCTGAAGAAATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCTGGGAGATTGGGGCAGGACCCCAGCGGGCACCGCTGGGG‑TGGTTTGAAAAggggggggacaccatggg                                                                                                                                                                                  >  2:79166/1‑235 (MQ=255)
                                                                          ggTATCTGAAGAGATGGCGGAAATTTACCGTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCAGTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGTTATTg                                                                                                                                                                 <  2:140083/251‑1 (MQ=255)
                                                                              tCTGAAGAGATGACGGAAATCTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGCGGCAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTATCACAGATTTGCGCACGCACCGTGCCGGCACCCCTGGGG‑TGCTTTGTACCACCGCCGGCAGCACTCGTTGCAAGCATAGGTATTGGTTg                                                                                                                                                             >  2:249809/1‑251 (MQ=255)
                                                                                 gAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAgg                                                                                                                                                          <  1:15292/251‑1 (MQ=255)
                                                                                          gCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGGATTGGTTGAGGAACGCGCa                                                                                                                                                  >  1:15629/1‑250 (MQ=255)
                                                                                           cGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAAt                                                                                                                                                <  1:9002/251‑1 (MQ=255)
                                                                                                        ggAACGCAATATGCAGGTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTTAGGAACGCGCCATGACGATcatccag                                                                                                                                   <  2:41/250‑2 (MQ=255)
                                                                                                                atatGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCCGATTGGGGCAGGCCCCGGGCGGGCACCGCTGGCG‑GGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGGATTGGGTGAGGAACGCGCAATGACGATCATCCATCCTCTGCt                                                                                                                           >  1:92895/1‑251 (MQ=255)
                                                                                                                          gCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCTGGCAGATTGGCGCAGGCACCGGGCGGGCACCCCTGGCG‑GGCTTTTAACAGGCGCTGGGAGCACTGGCTGAAAGCAAGGGGATTTGGTGAGGAACGCGCAATGACGCTCATCCATCCTCTGCTTGCCagtagt                                                                                                                 >  1:219737/1‑251 (MQ=255)
                                                                                                                             gATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGAGTGGCGCAGGCCCCCTGCGGGCCCCCCTGGCG‑GGCTTTGAAAAGGCGCTGGGAGCACTGGCTGAAAGCATGGGGATTGGGTGGGGGAAGCGCAATGACGCTCCTCCATCCTCTGGTTGCCcgtaggagg                                                                                                              >  2:51741/1‑247 (MQ=255)
                                                                                                                                  cAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCCCCGTGCGGGCACCGCTGGCG‑GGCTTTGAACAGGCGCTGGGAGCACTGGCTGAAAGCCTGGGTATTGGGTGAGGGACGCGCAATGGCGATCATCCATCCTCTGCTTGCCcgtaggaacggacc                                                                                                         >  1:171972/1‑242 (MQ=255)
                                                                                                                                    aGCTGGGATTATCAGGGCGCGGGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGAATGGCGCGGGCAACGTGCGGGGCCCGCTGGGG‑GGCTTTGTACCGGCGCGGCAATCACTGGGTGAAAACATGGGGATTGGTTGCGGGACACGCGAGGACGAGCATCCCTCCTCTGCtttcacggagtagcgccccg                                                                                                        >  2:165296/1‑232 (MQ=255)
                                                                                                                                        gggcttttCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCGTGCCAGTAGTAGCGCACCGAAtt                                                                                                    <  2:59254/246‑1 (MQ=255)
                                                                                                                                        gggATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAAtt                                                                                                    <  1:177139/250‑1 (MQ=255)
                                                                                                                                                          gCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTAGCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGt                                                                                  <  2:229372/250‑1 (MQ=255)
                                                                                                                                                              ggggCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATATGGAAGATTGGGGCAAGCAACGTGCGGGCACCGCTGGCGGTG‑GTTGAACAGGCGCCTGAAGCACTGGCCGAAAGCATGGGGATTGGTTGAGGAACAAGCAAAGACGATCATCCATCCTCTGCTTGCCCGTCATAGCGCCACGAATTATTGCCAGTCCTGGGGGTTTGCg                                                                             >  1:150925/1‑251 (MQ=255)
                                                                                                                                                                 gCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCCGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAAAAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGCATTATCGCCAGTCCTGGCGGTTAGCgggg                                                                          >  1:34354/1‑250 (MQ=255)
                                                                                                                                                                                      aGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCCGGAAGCACTGGCTGAAAGCATGGGGCTTTGTTGAGGAACGCGCAAAGACGATAATCCATCCTCTGCTTGCCCGGAGTAGCGCACCGAATTATCCCCCATCCTGGCGGTTAGCgggggggtgggggggggggggtta                                                      >  1:37252/1‑229 (MQ=255)
                                                                                                                                                                                                                  cccGGGATCGCCCATAAAGAGGCGGGGATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAAGTAGCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGTGCGATTAACCTGATGACGGAAAGCGGAGAACTTTTaa                         <  2:200066/250‑1 (MQ=255)
                                                                                                                                                                                                                                         ggggATCTGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGGGTGTGGCGGCGGGCGGTTAACCTGATGACGGGAAGCGGCGAAATGTTAACGTTGCCTCGTCAggggaggggg  >  2:219616/1‑244 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    
GGAGGTGCGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCG‑TGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGGGAGTGTGGCGGCGGGCGATTAACCTGATGACGGAAAGCGGCGAACTGTTAACGTTGCATCGTCAGGGTAGTGGT  >  NC_000913/549287‑549770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑