Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,330	G→A	L3L (CTG→TTG)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,330	0	G	A	100.0%	65.5 / NA	20	L3L (CTG→TTG)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

GAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCC  >  NC_000913/3507097‑3507567                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              gagTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCCTACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCtt                                                                                                                                                                                                                              >  2:183992/1‑251 (MQ=255)                     gcCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATc                                                                                                                                                                                                           >  1:134012/1‑250 (MQ=255)                              tCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGa                                                                                                                                                                                                  <  1:64125/250‑1 (MQ=255)                                         gagaTCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCATGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCTCCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTGATTTCAGATGGTTAATCAGCATCGGAATCGCGTGGTCg                                                                                                                                                                                      <  2:141267/251‑1 (MQ=255)                                          agaTCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGAAGCCGTCATGAAAAACAGCCGCCGAGCGATGGGCCAGAAGCGATGTCATACCCGTCCGGCACGCCACCACGATAATCTGGAACTACAAATCCATAGTTCTGCCTTTTATTTCAGTCTGTTAAACAGGATCGGAAACGCGTGTTCg                                                                                                                                                                                      >  2:79212/1‑250 (MQ=255)                                                                                  agcaaTACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCAGCGGAATCGGGTGATCGACGTGCTCAACGGAAAGGCCAAACGCCGCTTTTCCTTCAGc                                                                                                                                             <  1:125255/247‑1 (MQ=255)                                                                                   gCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCg                                                                                                                                            <  1:48849/251‑1 (MQ=255)                                                                                       tACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTACTTCAGCGATc                                                                                                                                         >  1:38870/1‑250 (MQ=255)                                                                                                                ataGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGACGAGCGATGCGCTAGAAGCGATGTCATAACCGTCAGGCACGCCACCACGATAATCTGAAGATACAAATCCATTGGCGTGCCGTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGc                                                                                                               >  1:47603/1‑251 (MQ=255)                                                                                                                          aTCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGTCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACTTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCt                                                                                                     <  1:102298/251‑1 (MQ=255)                                                                                                                          aTCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGAGGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCAATTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCt                                                                                                     >  2:217787/1‑251 (MQ=255)                                                                                                                             aGTTGCGGCAGGAGCGGGCGGATGCCGTCCTGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCTTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGt                                                                                                  >  2:174506/1‑251 (MQ=255)                                                                                                                                tGCGGCCGGAACGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTCCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGttt                                                                                                <  2:54807/250‑1 (MQ=255)                                                                                                                                        gATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGt                                                                                       <  2:47466/251‑1 (MQ=255)                                                                                                                                                  aTGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCATTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                             <  2:123682/251‑1 (MQ=255)                                                                                                                                                  aTGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                                             >  1:173647/1‑251 (MQ=255)                                                                                                                                                                                                                      cacGATAATCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGt          >  2:49361/1‑250 (MQ=255)                                                                                                                                                                                                                             aTCTGAATATACAAATCCATAGTCGTGCATTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGACAAACGCCACTTTTCATTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTAAACGCGCCGATGTAATAATcc  >  1:86601/1‑251 (MQ=255)                                                                                                                                                                                                                              tCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACt                                                                                                                                                        <  1:210028/100‑1 (MQ=255)                                                                                                                                                                                                                              tCTGAATATACAAATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACt                                                                                                                                                        >  2:210028/1‑100 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              GAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCACCGGTATTACACGCGCCGATGTAATAATCC  >  NC_000913/3507097‑3507567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑