Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	95.0%	53.4 / NA	20	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/10);  minor base G (0/1);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTG  >  NC_000913/696255‑696715                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCATCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCa                                                                                                                                                                                                                      >  2:121382/1‑250 (MQ=255)                gTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCGCTT‑ACa                                                                                                                                                                                                     <  2:194088/251‑1 (MQ=255)                   gTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAg                                                                                                                                                                                                   <  1:216854/250‑1 (MQ=255)                      gCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGGGCACttcaaagggac                                                                                                                                                                                                >  2:228486/1‑242 (MQ=255)                                aTTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGaa                                                                                                                                                                                     <  2:80989/251‑1 (MQ=255)                                    ccGGATGCGTGCCACCCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAAACGGGCGCACTTTACT‑GGTAAGCGTCTTGAATaa                                                                                                                                                                                  >  2:157114/1‑250 (MQ=38)                                            gTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAAt                                                                                                                                                                                >  1:192457/1‑244 (MQ=255)                                            gTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAAt                                                                                                                                                                                <  2:192457/244‑1 (MQ=255)                                               ccAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCGGGTGCACTT‑ACAAGGTAAGCGCCTTTAATAAATTGGCTGGGGt                                                                                                                                                                      >  2:167921/1‑251 (MQ=255)                                                       aGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                                                                                                                              <  2:37714/251‑1 (MQ=255)                                                                                                     gaggtgtCAGCTTCGAATTCGACATTGAATGAACGCAGAAAAGAAAAACGGTCGCCGAAGCGAGCTTTGTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGTTGCCTTACCGATTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                                                                                <  2:5700/248‑1 (MQ=38)                                                                                                      aCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGACGCGCGATTTTTTACTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGACGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAAGGCCGGAATCAGCAACCGGTGCCTTACCTCTTgg                                                                                                               >  1:159398/1‑251 (MQ=37)                                                                                                                           cATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtt                                                                                          <  1:225819/251‑1 (MQ=255)                                                                                                                            atttgatgaaCGCAGACAAGCAAAACGCTCGCGGAAGCGAGCTTTTTTAATTTGGCTGTGGTACGAGGATTCGAACCTCGGAATGCCAGAATTAGAATCCGGTGCCTTCCCGATTGGATATACCCCAACTGGGTGCACTT‑ACGAGGTAGGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtt                                                                                          <  2:137478/245‑1 (MQ=25)                                                                                                                                             aaGCAAAAAGCTCGCGGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCGCTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGGCTTACCTCTTGGCGATACCCCAACAAAATGGTTTTGAATTTGCCGAACata                                                                        <  1:140524/251‑1 (MQ=38)                                                                                                                                               gCAAAAAGCTCGCCGAAGAGAGCTTTTTTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGAATTAGAATCCGGTGCCTTACCGCTTGGCGAAACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATAtt                                                                      <  2:84330/251‑1 (MQ=255)                                                                                                                                                 aaaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGACTGCCGGAATCAGAATCCGGTTCCTTACCGCTTGGCGATACACCAACAAATTGGTTTTGAATTTGCCGAACATATTCg                                                                    >  1:249750/1‑251 (MQ=255)                                                                                                                                                                  gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGGTGCCTTACCGCTTGGCGATACACCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACATCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATCGGTTTTGAATTTGCCGAACATATTAGACACATTCAGAATTtgg                                                   >  1:166306/1‑251 (MQ=38)                                                                                                                                                                                 ggCTGGGGAACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATAAATTCAGAATTTGGAGGCTACGACGGGAt                                    >  1:202107/1‑251 (MQ=255)                                                                                                                                                                                                                   ggAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGAGACATTCAGAATTTGGTGGCTACGACGGGATTCTAACCTGTGACCCCATCATTATGAGGGAtgtg  <  2:158525/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTT‑ACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTG  >  NC_000913/696255‑696715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑