Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,447	C→T	A690T (GCG→ACG)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,447	0	C	T	100.0%	95.7 / NA	29	A690T (GCG→ACG)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

GCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTG  >  NC_000913/3471220‑3471686                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                gCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTcc                                                                                                                                                                                                                           >  1:51632/1‑250 (MQ=255) gCCAGTACGGTGGTGATTGCAACGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCGTCAAGTACGGCCTGAGCAACGTTACGCGGCGTTTCATCATACTTCAGAAATTCCa                                                                                                                                                                                                                          <  2:98323/251‑1 (MQ=255)             gtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTatgatg                                                                                                                                                                                                              <  2:79184/251‑1 (MQ=255)                       cGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCttt                                                                                                                                                                                                    <  2:111832/251‑1 (MQ=255)                                   ttttACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACg                                                                                                                                                                                        >  1:237450/1‑251 (MQ=255)                                                     ggCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATc                                                                                                                                                                       <  2:66168/250‑1 (MQ=255)                                                                               ggTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAg                                                                                                                                            <  1:166128/251‑1 (MQ=255)                                                                                  tttGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGt                                                                                                                                         >  2:170268/1‑251 (MQ=255)                                                                                    ggTACGTTCAAATTGTTCTGTAGACACGGCTATATTCCTGACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACACTTAGGCGTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCGTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACtgccgcgtcg                                                                                                                                       <  2:55156/250‑10 (MQ=255)                                                                                                        tAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACtt                                                                                                                    <  1:26783/250‑1 (MQ=255)                                                                                                                  catatTCCTTACTATAGCGCTCTCTCCTTCAGGAGAGAGCACGGGCCTTTGGTATTATCCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACATTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCTGACAGCGGTACTTCAGCGTGGGTCTTAACGCCAGTAACTTCAGATTCCTg                                                                                                          >  2:4650/2‑250 (MQ=255)                                                                                                                   atatTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGa                                                                                                         <  2:25296/250‑1 (MQ=255)                                                                                                                       tCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCATGACCttt                                                                                                    >  2:10/1‑251 (MQ=255)                                                                                                                             cTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGCATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCAt                                                                                              >  1:234133/1‑251 (MQ=255)                                                                                                                                    gCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAGTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGGTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCacg                                                                                        <  2:199564/250‑1 (MQ=255)                                                                                                                                          cccTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACgg                                                                                 <  1:61593/251‑1 (MQ=255)                                                                                                                                              tCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTc                                                                              >  2:239372/1‑250 (MQ=255)                                                                                                                                                      gagCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCg                                                                     <  2:40300/251‑1 (MQ=255)                                                                                                                                                                     gTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCgg                                                        >  2:248653/1‑249 (MQ=255)                                                                                                                                                                        ttAACCATTAGGCTTATTTACCACGTGCTTCAATTACGGCTTGAGCACCGTTACTCGGCGTTTCATCATACTTCCGGACTTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGTCCTTTGAGCATACCACGACGACGGCTCAAGTAACCGATAACGTCACCGGCGTtc                                                   >  2:156546/1‑251 (MQ=255)                                                                                                                                                                                             cactggCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAAGAATTCCATAGTGTATGATGCACGACCGTTGGTCAGAGAACTCAGCTGAGTTTCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTTGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATACCGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCa                              <  2:84648/247‑1 (MQ=255)                                                                                                                                                                                             cACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCCCGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTAAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTc                               >  1:157894/1‑250 (MQ=255)                                                                                                                                                                                              aCGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCGGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCa                              >  1:201379/1‑250 (MQ=255)                                                                                                                                                                                                   cTTCAATTACGGCCTGAGCAACGTTACTCGTCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTc                        >  1:225352/1‑251 (MQ=255)                                                                                                                                                                                                        aTTACGGCCTGCGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGAt                   >  2:247320/1‑251 (MQ=255)                                                                                                                                                                                                             gtccTGAGCAACGTTAGTCTGCGTTTCAACATACTGTAGGAACTCCATTGTGTATGATGCACGCCCTTTGGTCAGATAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCTGTACTTCAGCGTTGATCTTAACGTGATTATCTTCAGATTCCTGACCTTTGAGCATACCCCTTCGACTTTTCAAGTCACCGCTATCGTCACCGGTTTGCTCTTCCGGAGTTTCTACTTAAACCTTCATGATCGGCt              <  2:10897/249‑1 (MQ=255)                                                                                                                                                                                                                 tGAGCAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCa            >  1:203076/1‑249 (MQ=255)                                                                                                                                                                                                                     cAACGTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGa      <  2:237450/251‑1 (MQ=255)                                                                                                                                                                                                                         gTTACTCGGCGTTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTg  >  1:134387/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAATTACGGCCTGAGCAACGTTACTCGGCGCTTCATCATACTTCAGGAATTCCATAGTGTATGATGCACGACCTTTGGTCAGAGAACGCAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTG  >  NC_000913/3471220‑3471686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑