| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,495,346 | C→T | A20V (GCC→GTC) | ydcJ → | DUF1338 domain‑containing protein YdcJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,495,346 | 0 | C | T | 96.3% | 82.5 / ‑6.3 | 27 | A20V (GCC→GTC) | ydcJ | DUF1338 domain‑containing protein YdcJ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/9); minor base G (1/0); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCG > NC_000913/1495115‑1495587 | gttTGATTATGATAACTTGATTGCAAAATATTACTAACAATTAAAGCAGTTATGTTACAGCAAAATGGCTAATATTGATGTTTTCGCGGCGAGATCACAGCTTGTAAATTCTTCCCGCAAGCGTGAATGCGGTTACCAACTCTCCAGATTCCTGACCACTGGAGGCAGCCACAATGGCGAACAGCATCACGGCGGACGCGATTCGGGAACAGTTTTCGCAGCAAATGTACGTCATGTACAAGCaagaaact > 2:236517/1‑248 (MQ=255) ttGATTATGATAACTTGATTGCCAAATATTATTACCAATTAAAGCAATTATGTTACCGCAAAATGGATAATATTGATGTTTTAGCGGCGAGATCACAGTTTGTAAATTCTTCACGCAAGAGTGATTGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGCGATTCGGGGACCTTTTTCGCAGGCCATGGCAGTCCTGTACCAGCAAGAAATTcc > 2:169020/1‑251 (MQ=255) tATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCCGCAAGAAGTTCCGCAat > 2:40927/1‑251 (MQ=255) aTAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATgg > 2:75919/1‑251 (MQ=255) cTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACg > 2:105519/1‑251 (MQ=255) tGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGGCAGTCATGTAACAGCAAGAAGTTCCGCAATAGGGCACgct > 1:7106/1‑251 (MQ=255) attaACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGc > 1:166178/1‑251 (MQ=255) aCAGCAAACTGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTgg > 1:57667/1‑250 (MQ=255) cggCAAAATGGGTAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGAACGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGCTGAGATTCGGGAACAGTTTTCGCACGCAATGTCCGTCATGTACCAACAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCCGATGTGTATGTGGcatgtcggga > 2:244262/3‑241 (MQ=255) gATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTCAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACAATCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATTTCAGTCCTGTACCAGTAATAAGTTCCGCACAATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCGTGCTTTGCTGGACAAACA‑TCCTc > 2:49864/1‑251 (MQ=255) cgcgGCGAGATCACAGTTTGTAACTACTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCCGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCTCGCTGCTGGCACTGGGAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATg > 2:244482/1‑251 (MQ=255) cggcgAGATCCCAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATCTGGCACGCTGCTGGAACTGGTATCTGATGTTAATCTGGCTGTGCTGGA‑AAACAATCCTCCACTTCACTAAAAAAttgt > 2:31093/1‑248 (MQ=255) gagaTCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCTCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑TAACAATCCTCAACTGCACGAAAAAATGGTAAAt < 1:19638/251‑1 (MQ=255) gagaTCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAAt > 1:1273/1‑251 (MQ=255) aTCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCGCGAAAAAATGGTAAATGCa < 2:156910/251‑1 (MQ=255) aaaTTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGgcg > 2:26340/1‑251 (MQ=255) ccGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAAt < 2:197282/251‑1 (MQ=255) gCAAGAGTGAATGCGGTTACCTGCACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGGTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGt < 2:76782/251‑1 (MQ=255) ccAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTgg < 1:218103/251‑1 (MQ=255) gACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTcg > 1:68219/1‑235 (MQ=255) gACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTcg < 2:68219/235‑1 (MQ=255) cacTATGGCGAACAGCATCACGGCTGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTTGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTc < 1:8553/251‑1 (MQ=255) cGAACAGCATCACGGCGGATGAGAGTCGGGAACAGTTTTCGCAGGCAATGGCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATg < 1:240068/251‑1 (MQ=255) cATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGccc > 2:161308/1‑250 (MQ=255) tctcggggggagagagtagagaagaGTTTTCGCAGGATATTTGAGGCATGTACTAGCAAGAAGTTCCGCAATATGGCACGCGGCTGGAACTAGTAGCTGATGTGAATGTGGCTGTGCTGGA‑AAACAATCCTCAACTGCATGGAAACATGGGGAATGCACACACGCTGGCGTGGTTGAACGTTTAAAGTCATGGGGCGATTGGCGTTGGGACTGCCCAGGAGCTTGCGACTCTTCGGCGGTTGTGTGCCAtt > 2:32233/25‑251 (MQ=255) gAACAGTTTTCGCAGGCAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTg > 2:199255/1‑249 (MQ=255) cAATGTCAGTCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCg < 1:119715/251‑1 (MQ=255) | GTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCACGCTGCTGGAACTGGTAGCTGATGTGAATCTGGCTGTGCTGGA‑AAACAATCCTCAACTGCACGAAAAAATGGTAAATGCAGACGAGCTGGCGCGACTGAATGTTGAACGTCATGGGGCGATTCGCGTTGGGACTGCACAAGAGCTTGCTACTCTTCGGCGGATGTTTGCCATTATGGGGATGTACCCGGTGAGCTATTACGATCTCTCG > NC_000913/1495115‑1495587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |