Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,658	G→A	P95S (CCC→TCC)	yqaA ←	DedA family protein YqaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,658	0	G	A	100.0%	54.3 / NA	18	P95S (CCC→TCC)	yqaA	DedA family protein YqaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

GATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACT  >  NC_000913/2816423‑2816901                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    gaaacttcggggATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTtaatag                                                                                                                                                                                                                                      <  2:119045/242‑2 (MQ=255)                                tGTCAAAATCGTTTTAATTAGCATAACGGTAATGGTAACCATATGTGCAGGCCTACAATTAGGGCCCCCACATCATGCCCTGAACGGTCGCTGCTCCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATCCGCATCCATCCCGCTAACAGACCCAGTAAATCGCCAACACCGGACATCCACCTTAATATTAGCTTACCTGCACCATAGCGTTTcagcc                                                                                                                                                                                                         >  1:202605/1‑249 (MQ=255)                                                                              gCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCCTCCCGCTAACAGACACAGTAAAGCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGgaaag                                                                                                                                                          <  2:10826/250‑1 (MQ=255)                                                                                           tAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGa                                                                                                                                             <  1:50902/250‑1 (MQ=255)                                                                                             gTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATaa                                                                                                                                          <  2:36888/251‑1 (MQ=255)                                                                                                       atcatGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACgttagtta                                                                                                                                 >  1:193496/1‑250 (MQ=255)                                                                                                                ggAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTGACCAAGGCTCAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCCCGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATATCGTTAGTTAACCCTACAAg                                                                                                                       <  2:189264/250‑1 (MQ=255)                                                                                                                                     gcaacATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTg                                                                                                   <  1:225331/250‑1 (MQ=255)                                                                                                                                                            aaGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGAc                                                                           >  1:196014/1‑251 (MQ=255)                                                                                                                                                               gCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTg                                                                         >  1:246511/1‑250 (MQ=255)                                                                                                                                                                                 cGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGGCTGATCCCGGAAAGCAACATTg                                                      <  2:95721/251‑1 (MQ=255)                                                                                                                                                                                         aCGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGc                                              >  2:47198/1‑251 (MQ=255)                                                                                                                                                                                                 cgcATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTc                                      <  1:108442/251‑1 (MQ=255)                                                                                                                                                                                                  gcATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACAACTTCg                                     >  1:75147/1‑251 (MQ=255)                                                                                                                                                                                                       catccCGCTAACAGACACAGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGtt                                <  2:202605/251‑1 (MQ=255)                                                                                                                                                                                                                       acaGTAAATCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAgtgt                 >  1:32498/1‑250 (MQ=255)                                                                                                                                                                                                                              aTCGCCAACCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTATTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTgc         >  2:189520/1‑250 (MQ=255)                                                                                                                                                                                                                                     aCCACGGACATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTTAGAAAact  <  1:47198/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    GATACGTCCGGGATCAAATTGACCTCCCGCCTGTCAAAATCGTTTTAATTAGCATAACTGTAATGGTGACCATATGTGCAGGCCTACAATTAGTGCCACCACATCATGCCCTGAACGGTCGCTGCTGCAACTGCAACATAGCGTAACGCTTTACCAAGGCACAAAAAAAAGATTACCGGTCCCCACGAGATGCGCATCCATCCCGCTAACAGACACAGTAAATCGCCAACCACGGGCATCCAGCTTAATAATAGCGTGACTGCACCATAGCGTTTCAGCCAGCCGGTAGCTTTCTCTTGCCAGCGCGATGTTTTACGCAATGGAAAGAAACGCCCAAGGATAACGTTAGTTAACCCTCCAAGGCTATTACCCATTGTTGCTGTTAAAACTAAAACCCAGGGATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACT  >  NC_000913/2816423‑2816901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑