Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,556,573	C→T	intergenic (‑293/‑52)	bdm ← / → osmC	biofilm‑dependent modulation protein/osmotically inducible peroxiredoxin OsmC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,556,573	0	C	T	100.0%	61.4 / NA	20	intergenic (‑293/‑52)	bdm/osmC	biofilm‑dependent modulation protein/osmotically inducible peroxiredoxin OsmC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/7);  total (13/7)

GGCAAATATTTGCCGCTTCGTTGTTAAGATTAGTCCTGGTTGATGATTTTTATATTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGCTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTC  >  NC_000913/1556351‑1556806                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          ggCAAATATTTGCCGCTTCGTTGTTAAGATTAGTCCTGGTTGATGATTTTTATATTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCtt                                                                                                                                                                                                               >  2:72165/1‑251 (MQ=255)   cAAATATTTGCCGCTTCGTTGTTAAGATTAGTCCTGGTTGAGGATTTTTATATTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCtttt                                                                                                                                                                                                             <  2:192169/251‑1 (MQ=255)                                 gTCCTGGTTGATGAATTTTATATTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATc                                                                                                                                                                               >  2:178416/1‑251 (MQ=255)                                                   tataTTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGc                                                                                                                                                              <  2:220057/250‑1 (MQ=255)                                                                cATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCg                                                                                                                                                >  2:201905/1‑251 (MQ=255)                                                                                               aTCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAg                                                                                                                                                                                                  <  1:142264/170‑1 (MQ=255)                                                                                               aTCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAg                                                                                                                                                                                                  >  2:142264/1‑170 (MQ=255)                                                                                                                              gTTATATCTTCCCGGTAATCTATTGTGGGCATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGATCCACACTGACAATCCATAAGCAAGTTCAGTCACACTGGGAAGGCGATATCAAACGCTGGAAGGGAACAGTATCCACCGAGAGTGGCGTTCTTTACCAACAGCCGTAt                                                                                  >  2:206663/1‑251 (MQ=255)                                                                                                                                 atatatTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAAcgc                                                                                                                                     >  1:239961/1‑197 (MQ=255)                                                                                                                                 atatatTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAAcgc                                                                                                                                     <  2:239961/197‑1 (MQ=255)                                                                                                                                    tatTCCCGGAAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGACCCAACAGCCGGCTGGAttt                                                                            >  2:72751/1‑251 (MQ=255)                                                                                                                                            gTAATCTGTTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAAAACACGAATGGATTTAACAcgcg                                                                    >  2:167771/1‑251 (MQ=255)                                                                                                                                                     tgtgGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAg                                                            <  2:108204/250‑1 (MQ=255)                                                                                                                                                                      aGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGaaccaacc                                          >  1:93154/1‑251 (MQ=255)                                                                                                                                                                      aGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGGATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGaaccaacc                                          >  1:36983/1‑251 (MQ=255)                                                                                                                                                                              aGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTgaagaa                                  <  1:208126/251‑1 (MQ=255)                                                                                                                                                                                   tatTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGACAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAAACAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACTGAt                             >  1:147900/1‑251 (MQ=255)                                                                                                                                                                                          gCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACTGATTGgcgc                       >  1:103419/1‑250 (MQ=255)                                                                                                                                                                                                   tATTCGGAATATCCTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACTGATTGGCGCAGCGCCTGcc             >  2:211813/1‑251 (MQ=255)                                                                                                                                                                                                               ccTGCTTATCCTCGTGTTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACGGATTGGCGCAGCGCATGCCGCATGTTtctc  <  2:217389/250‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                          GGCAAATATTTGCCGCTTCGTTGTTAAGATTAGTCCTGGTTGATGATTTTTATATTTTAACACCATGATATTCATAGGGATTGTGATTGGTATGATCCGATTAATATTGATACAATATCTTTTGGGTTATATATTCCCGGTAATCTATTGTGGGAATTTAATTTAAGTGCAGAAGTAATATTTTCGCCGGATTTTATTCGGAATATCCTGCTTATCCTCGTGCTGTTTCTCACGTAGTCTATAATTTCCTTTTTAAGCCCACAGGAGAGCAACAATGACAATCCATAAGAAAGGTCAGGCACACTGGGAAGGCGATATCAAACGCGGGAAGGGAACAGTATCCACCGAGAGTGGCGTGCTGAACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAACCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTC  >  NC_000913/1556351‑1556806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑