| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,549,497 | G→A | P192S (CCG→TCG) | malQ ← | 4‑alpha‑glucanotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,549,497 | 0 | G | A | 95.5% | 63.6 / ‑3.4 | 22 | P192S (CCG→TCG) | malQ | 4‑alpha‑glucanotransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/10); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01 | |||||||||||
GCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTC > NC_000913/3549257‑3549738 | gcgcGAAACCTTTCCACGCCAGTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCa < 1:167307/251‑1 (MQ=255) aaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCTC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATc < 1:166996/251‑1 (MQ=255) ccATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TTAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGcc > 2:171241/1‑250 (MQ=255) aTTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAAt < 2:142592/251‑1 (MQ=255) aTTCGTAAGGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTGGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAAt < 2:82318/251‑1 (MQ=255) aaTGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGa < 1:133490/251‑1 (MQ=255) gggCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGcc > 2:207055/1‑251 (MQ=255) gggCGGTAACCGTGGAGAAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGcc > 1:206517/1‑251 (MQ=255) aaTCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCt > 1:88922/1‑251 (MQ=255) cccTGTTGCAGCGTCGTTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGAGGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAAt < 1:80255/250‑1 (MQ=255) ggTTGCAGCGTTTGTTGAGTGTTCGGCAACTGCCACCAGGCATGAGCCTCTT‑GCAGAAGATGAAAATCTTCAACGGCGTAAACGTCGATAAAAATCACATCCACCCAACGCCGGGAAGAGGGGCTGTAGGGCCGGGCGTACTCCGGATTGGCCGATTAGAGCCAATAAAGCGGTCTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATAcc < 2:125497/250‑1 (MQ=255) gTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGttttt > 2:181874/1‑251 (MQ=255) ccaccaGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGCCGTTAAAGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGATGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGGTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCt > 1:21836/1‑251 (MQ=255) gCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGCTATAAATCACATTCAGCCAACGGCGAGCAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGc > 2:120562/1‑250 (MQ=255) tCAACGGCGTTAACGTGGATATAAAGCACATTCAGCCAAGGGCGAGAAGACGGGCTGGAGGGGCGGGCGCTCTCCGGATTGGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCg < 2:50209/200‑1 (MQ=255) tCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCg > 1:50209/1‑200 (MQ=255) taAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCg > 2:76608/1‑251 (MQ=255) ccaTTCAGCCAACGGGGAGAAGCCGGGCTTTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGGGCTCATGATTCGGGTTCAGGCCAATGACCGCCCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCgg < 1:213104/250‑1 (MQ=255) aagaCGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCa > 1:121804/1‑250 (MQ=255) agaCGGGCTGTAGGGGTGGGCGCCCTCTTGATTCGCCGAAAAGAGCGCATGAATCGGGTCCAGGCATGTGACCGCCCCGCCACGTTTTGCCACATCCACCAGCAGCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCTCAGCGGATAAAGCTGAACGCAGGCACCCCACAGCGCTTGTTTATTCAGCAACGCCTGCTGTTCGTAACAGCGTTTCGGGTCGATAATCACCCGGCAAt < 2:21836/251‑1 (MQ=255) gTATGGGCTGGCGCTCTCCGGATTTGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACACGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCt > 1:244347/1‑251 (MQ=255) tttGCCGAATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTc > 1:69435/1‑249 (MQ=255) | GCGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCGCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTTCGC‑TAAGATGGAAATCTTCAACGGCGTTAACGTCGATATAAATCACATTCAGCCAACGGCGAGAAGACGGGCTGTATGGGCTGGCGCTCTCCGGATTTGCCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTC > NC_000913/3549257‑3549738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |