Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,612,191	G→T	G98* (GGA→TGA)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,612,191	0	G	T	100.0%	101.1 / NA	31	G98* (GGA→TGA)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/15);  total (16/15)

ATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGA  >  NC_000913/2611948‑2612419                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      aTTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGc                                                                                                                                                                                                                                <  1:108551/251‑1 (MQ=255)          gTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACg                                                                                                                                                                                                                       >  1:9600/1‑251 (MQ=255)            aaaCGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCt                                                                                                                                                                                                                     <  1:178713/251‑1 (MQ=255)                ggAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTCCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTAt                                                                                                                                                                                                                 >  2:169257/1‑251 (MQ=255)                                      aacaGAAACACGGCAAGGCGTCTGTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGGACAACGTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGtttt                                                                                                                                                                                           <  2:220109/250‑1 (MQ=255)                                                   cAAGGCGTCTTTATAACGCGCCTTCATCCCGCTGCCGCCGCCGATCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGAtt                                                                                                                                                                              >  2:162316/1‑251 (MQ=255)                                                    aagggggatgtactacgctctctCATCCCGCGGCCGTCGCCTGTCAGCCCGGTGCAACTTATTGAACTGCATGTTCGCAAAAGCAAATTCTATTACGGCTTCCAGGATGATGGCGGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGAGTATTCGCGTCACGCCGTCGTTCGCGGCAGTAGTTGGATGGCGGAGCTACGCGTGATTCGCACCCAGGGCAGTCGTTTTGGGTCCCAGGATTg                                                                                                                                                                             <  2:237647/229‑1 (MQ=255)                                                    aaGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTg                                                                                                                                                                             >  1:235821/1‑251 (MQ=255)                                                     aGGCGTCTTTATTACGCGCATTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACACCTTCTTGCACTGCATGTTCTCACAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGc                                                                                                                                                                            >  2:156495/1‑251 (MQ=255)                                                                          tCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACtggctggc                                                                                                                                                        <  1:169257/250‑1 (MQ=255)                                                                              cccGCTGCCGCCGCCGTTCAGCCCGGTTCAACGTATTTAACTGCATGTTCTCAAAAGCACCTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCTGATCTACTCTTTATTCGCTCCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGa                                                                                                                                                   <  2:221681/251‑1 (MQ=255)                                                                                                   cccGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAAt                                                                                                                             >  1:142934/1‑251 (MQ=255)                                                                                                               tATTGAACTGCATGTTCTCACAAGAAACTTCTATTACCGCTACCATGATGATGGCAGAGATGTGACGGCAACAACAGAGTCTCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGCGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAatg                                                                                                                 >  2:251715/1‑251 (MQ=255)                                                                                                               tATTGAACTGAATGTTCTCAAAAGCATCTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAAAAACAGAGTATCAGGGCTAGATGGTCGATTATTCGCGTCACGCCGTCCTTCGCGGCAGTAGTTGAATGGCGGGGCTACGCTTTATGCTTTCCCGCGGCAGTCGTTTTACTTCCCAGGATGGTACACTGTTTAACTGTCTTGCGCGAAGAGTCTCCGTCGGT‑CTGCAATCATGGCTcca                                                                                                                   <  2:111402/249‑3 (MQ=255)                                                                                                                 ttGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAatgat                                                                                                               >  2:204707/1‑251 (MQ=255)                                                                                                                            gTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAAcagc                                                                                                     <  1:193320/250‑1 (MQ=255)                                                                                                                            gTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAAcagc                                                                                                     >  1:227685/1‑250 (MQ=255)                                                                                                                              tctcAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAAcagcag                                                                                                   <  2:55696/250‑1 (MQ=255)                                                                                                                                                ttACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTgg                                                                                >  1:212049/1‑251 (MQ=255)                                                                                                                                                            tgatgatgGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATcgcg                                                                    >  1:49890/1‑251 (MQ=255)                                                                                                                                                                      aGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGACAGATCGCGATCATCATCg                                                          >  1:138949/1‑251 (MQ=255)                                                                                                                                                                         gATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCCCGGCAGTCGTTTTACTTCCCAGGATTGCCCACTGTTTACCTGTCTGGCGCGGATCATCACC‑CCGGTTCTGCAATCATGGCTCAATGATTACGAACATCAGGTGGCGCTTCGTTTGCTGGCGAACGATCGCGatcatcctctggt                                                       >  2:41439/1‑244 (MQ=255)                                                                                                                                                                              gACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTgg                                                  <  1:154658/251‑1 (MQ=255)                                                                                                                                                                                  gCAACAACAGAGTATCAGGGCGAGATGGTCCATTATTCGCGTAACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTGCGCTTTATTCGCCCCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATCATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCCTCATCGGGTACTGGTTg                                               >  1:111553/1‑250 (MQ=255)                                                                                                                                                                                    aacaacAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGata                                            <  1:229064/251‑1 (MQ=255)                                                                                                                                                                                           gagTATAAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTa                                      >  2:241449/1‑250 (MQ=255)                                                                                                                                                                                                         gaTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGTGTACTGGTTGATATTACTAATGCAGTGCTGTcac                       <  1:158591/251‑1 (MQ=255)                                                                                                                                                                                                          aTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGAGATTACTAATGCAGTGCTGTcaca                      <  2:160753/251‑1 (MQ=255)                                                                                                                                                                                                                 attattCGCGTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGAt               <  2:138949/251‑1 (MQ=255)                                                                                                                                                                                                                       cgcgTCACGCCGTCCTTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTcgac         >  1:238295/1‑251 (MQ=255)                                                                                                                                                                                                                              cGCCGTCCGTCTCGGCAGTAGTTGAATGGCGGAGCTACGCTTTTTTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTGAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCGCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGAGCATCATCGGGGACTGGTTGATATTACTAATGCAGTGCTGTCACATTTTGACCTCGACGGTCTGa  <  2:117139/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      ATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACC‑CCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGA  >  NC_000913/2611948‑2612419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑