Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,882,226	C→T	N105N (AAC→AAT)	dmlA →	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,882,226	0	C	T	91.7%	29.6 / ‑6.1	12	N105N (AAC→AAT)	dmlA	D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/3);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACAC  >  NC_000913/1882021‑1882467                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   ccTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGACATGAGCACCTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGCTACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCg                                                                                                                                                                                                       >  2:122491/1‑251 (MQ=255)                                    gAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGTAAACAGGCTGGCGACCTCGATTTTTAAGTggg                                                                                                                                                                    >  2:62430/1‑250 (MQ=255)                                                                                        ttAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAAAAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACAACGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGtgaatga                                                                                                                >  1:240598/1‑250 (MQ=255)                                                                                               tttCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGTGAATGAAgggac                                                                                                          >  1:7305/1‑246 (MQ=255)                                                                                                                            gCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCt                                                                                                                                                                                                           >  1:131532/1‑124 (MQ=255)                                                                                                                            gCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCt                                                                                                                                                                                                           <  2:131532/124‑1 (MQ=255)                                                                                                                                    gATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAATGCGAATATTCCTCGCT‑CGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACccg                                                                   <  1:230314/251‑1 (MQ=255)                                                                                                                                                              gtgGGTTTCGCGGCTGAAATTTCGTCGGGAATCCGCCCGGTCCCTCAATCGGCGCCCGGTTCTTCTCTTTCCGGGCGTTCCCTGCCCGCTGGCGGAAAAACAGCCTGGCGACACCGATTTTTACGTGGTCAGGAAAAACACCGAAGGCGAATATCCCTCGCT‑CGGCGGTAGAGTGAAGGAAGGTACAGAGCAGGAAGTCGTCATTCAGGAATCGGTTTTTCCCCGCCGTGGTGTCGATCGCATTTTGCGtt                                         <  1:194130/251‑1 (MQ=255)                                                                                                                                                                       gctgctGAAATTTCGTCGTGAATTCGACCAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGGCATCGGTTTTTACGTGGGCAGGGGAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGGGAATGAAGGTACAGAGCATGCAGTCGGCATTCCGGAATCGGGATTTACCCCCCGGGGTGTCGATTGCATTTTGAGGTATGCCtttc                                >  2:170218/1‑249 (MQ=255)                                                                                                                                                                          gttaaagtttCGTCGTGAAGCCGACCAGTACGTCATCCTGCGCCCGGGTGGTTCCTTTCCTGCCTTTCCCTGCCCGCTGGCGAGAAAACAGCCTGGCGACTTCTTTGTTTGGGTGGTCGGGAAAAACCCCGACGGCTAATAC‑CCCCCCTGCGGCGTTAGAGTGATTGAGGTTACAGGGCTTGATGTCGTCTTTCAGGATGCGGTATTTCACCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAAc                             <  2:128669/244‑1 (MQ=255)                                                                                                                                                                                                   cAGTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGac    >  1:82968/1‑251 (MQ=255)                                                                                                                                                                                                     gTACGTCAATCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCAATTTTTACGTGGTCCGGGAAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGGGGGAATGAAGGGACAGAGCCTGAAGTCGTCATTCAGGGATCGGGATTTACCCGCCGTGGTGTCGATCGCCTTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCCCGTCAGaaac  >  2:128381/1‑248 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   CCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCT‑CGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGTCCACGTAAGACAC  >  NC_000913/1882021‑1882467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑