Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,994	G→A	G44E (GGG→GAG)	cycA →	D‑serine/alanine/glycine/:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,994	0	G	A	100.0%	47.9 / NA	18	G44E (GGG→GAG)	cycA	D‑serine/alanine/glycine/:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/11);  total (7/11)

TTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGC  >  NC_000913/4429747‑4430225                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         ttCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACCACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGCTGCC‑TTTGTACGGGGTTGTTTTTgggttc                                                                                                                                                                                                                                       >  2:76368/1‑248 (MQ=255)                gTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCCTTGGGACGGGGGTGTTTTTGGAGTCTGGCCAAACGAtt                                                                                                                                                                                                                          >  1:190131/1‑249 (MQ=255)                gTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTa                                                                                                                                                                                                                         <  1:136930/250‑1 (MQ=255)                                    cccGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                                                                                                                                                                                    <  1:236709/251‑1 (MQ=255)                                                         taTAGACTGAATAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGGACGGGGGTGTTTTTGGAGTCTGGCAAAACGATTAGCCTTGGCGGGCCGTCGATCATTTTCGTTTATATGTTCATTg                                                                                                                                                                               >  1:171265/2‑251 (MQ=255)                                                         cATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCCTTGGTACGGGGGTGTTTATGGAGTCTGGCAAAACGATTAGCCTTTCCGGGCCGTCGATCCTTTTCGTTTatattttcattt                                                                                                                                                                               >  1:171258/1‑243 (MQ=255)                                                                     aaaGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctc                                                                                                                                                                    <  1:102946/250‑1 (MQ=255)                                                                                                          gaagaaAAAAACATGGTAGATCAGGTAAAATTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTCCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCATTTTCG‑TGATGCGGGCAATTGGGGAATTGCTGCTTTc                                                                                                                             <  2:244923/251‑1 (MQ=255)                                                                                                                                  gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAg                                                                                                     <  1:33990/251‑1 (MQ=255)                                                                                                                                           gTTGCAGATGATCAGGCCCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGACATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGGGACTTCGc                                                                                            <  2:47508/251‑1 (MQ=255)                                                                                                                                                         ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCg                                                                              <  2:62908/251‑1 (MQ=255)                                                                                                                                                                   aaaCAGTCGCTTCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑AGCCCTTGGTGCCGGGCCGTTTATTGAGCCTGGCAAAACCGTTAGCCTTGACCGGCCGTCCATCATTGTTGCGTATATGATTATTTGTTTTATGCTCTTTTTCGGTG‑TGCGCGTCATGGGGGTATTGTTGCTTTCGGATTTGGCATAAAAATCTGTTTGTGTTTTCGCTTCCGATTTAATGGGGGCGTgggg                                                                    >  2:135198/2‑250 (MQ=255)                                                                                                                                                                        gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGat                                                               <  2:6270/251‑1 (MQ=255)                                                                                                                                                                                            caAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTAc                                           <  2:41668/251‑1 (MQ=255)                                                                                                                                                                                               aCCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGAGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTACGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGAGGAATTGCTGCTATCGAATCAGAAATACAAATATTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTgg                                        <  2:162046/251‑1 (MQ=255)                                                                                                                                                                                                ccGACATATTCAGCTTATTGCCATTGGCGG‑CGCCCTTGGTACCGGGTTGTTTTTGGAGTCTGGCAAAAAGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATTGTCCTTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGt                                       >  1:233644/1‑251 (MQ=255)                                                                                                                                                                                                                     cATTGGCGG‑TGCCATTTGTACGGGGGTGTTTATGGAGTCTGGCAAAACGATTAGACTTGCCGGGCCATCGATCATTTTCGTTTATATGATCATTTGTTTTATGCTCTTTTTCG‑TGATGGGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGCTTTACTCTGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAAACGGtt                  >  1:192630/1‑250 (MQ=255)                                                                                                                                                                                                                                   aTTGGTACGGGGTTGTTTATGGAGTCTGGCAACACGATTAGCCTTGCCGGGCAGTCGATCATTTTCGCTTATATGCTCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACgtggt     >  1:141420/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggTACGGGGTTTTTTATGGAGTCTGGCAAAATGATTATGCGTGCCGGGCCGTCGATCATTTTCGTTGATATGATCATTGGTTTTATGCTCTTGTTCG‑TGATGCGGGCAATGGGGGAATTGCTGTTTTCGAATCTGGAATCCAAATCTTGTAGTGACTTCGCTTCCGAGTTACTCGGGCCGTGGGCAGGATAGTTCACCGGCTGGACTTTCTGGTTCGGCTGGGTTGTAACCTGTATTCCAGTCGtggtggg  <  2:96946/251‑2 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         TTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGG‑TGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCG‑TGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGC  >  NC_000913/4429747‑4430225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑