Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,161	C→T	F180F (TTC→TTT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,161	0	C	T	100.0%	81.4 / NA	27	F180F (TTC→TTT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

CTGGAAGGGCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGTGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGG  >  NC_000913/990937‑991402                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   cTGGAAGGGCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGTGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGGTTCCGAAACCGTGCTACCTGTTTGCGCt                                                                                                                                                                                                                          >  1:154609/1‑251 (MQ=255)   ggAAGGGCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGTGAAGCCGAAGGTTTCCGCCATATGACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCtgg                                                                                                                                                                                                                        <  1:232395/251‑1 (MQ=255)       gggCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGTGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGc                                                                                                                                                                                                                    <  2:429/251‑1 (MQ=255)                               cTTTGCACCCCGTGTGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGGTTCCGAAACCGTGCTAACTGGTTGCGCTGGTGGCAGGGGACTTTGTTGTACTgcggg                                                                                                                                                                                             >  2:235318/1‑248 (MQ=255)                                          gtgtGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCa                                                                                                                                                                                 <  1:91553/251‑1 (MQ=255)                                                aGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAA‑TCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTTGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTc                                                                                                                                                                          >  1:181130/1‑251 (MQ=255)                                                         tttCCGCCATATTACGTATTATCTCGACATCCCGGACGTCCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTAACTGTTTGCGCTGGGGGCCGTCGACTTTGCTGTACTGCGCGCTACCTTTACCAAGCGTTCTCGTcgcc                                                                                                                                                                  >  2:212488/1‑250 (MQ=255)                                                                   atTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCGGAAACCGTGCTACCTGTTTGCGCTGTTGGCAGGCGACTTTGATGTACTGCGCGATTCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACt                                                                                                                                                        <  1:157858/251‑1 (MQ=255)                                                                       cGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTTCGCTGGTGGCAGGCGACTTTGATGGACTGCGCCCTACCCTTACCACGCGTTCTGGTCGCGAAGTAGCACTgggg                                                                                                                                                    >  2:24565/1‑249 (MQ=255)                                                                         tattatCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAAACGTGCTACCCGTTTGCGCTGGGGGCAGGCGACTTTGATGTACTGCGCGGTACCTTTACCACGCGTTCTGGTCGCGCAGTAGCACTGGAGCt                                                                                                                                                  >  2:79493/1‑251 (MQ=255)                                                                             atCTCTACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGGCCCGTTTCCGAAACCGTGCTACCTGATTGCGCTTGTGGCAGGCGCCTTTGATGTACTGCGCGGTAACTTTCCCACGCGTTCTGGTCGCGAGGTAGAACTGGAGCTGtgc                                                                                                                                              >  2:72089/1‑249 (MQ=255)                                                                                                    gcgcGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTAACACGCGTTCTGGGCGCGAAGTAGCACTGGAGCTGTACGTCGATAGCGGCAACCTTGATcg                                                                                                                       >  1:193448/1‑251 (MQ=255)                                                                                                     cgcgTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTATAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATcgc                                                                                                                      <  2:179966/251‑1 (MQ=255)                                                                                                                        tattGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGAcc                                                                                                   <  2:29997/250‑1 (MQ=255)                                                                                                                                    aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTTGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCACCCTTTGTCGCGCGC‑CGTGGGCGCCGACCTCCCTGACAAAcc                                                                                      >  2:194052/1‑250 (MQ=255)                                                                                                                                    aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATcgc                                                                                                                      <  2:202760/220‑1 (MQ=255)                                                                                                                                    aaTCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATcgc                                                                                                                      >  1:202760/1‑220 (MQ=255)                                                                                                                                                          tCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTc                                                                                                 >  1:178182/1‑218 (MQ=255)                                                                                                                                                          tCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTc                                                                                                 <  2:178182/218‑1 (MQ=255)                                                                                                                                                                   aaCCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCt                                                       >  1:87274/1‑251 (MQ=255)                                                                                                                                                                                      aaatGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTGTCCGAAACCGGGCTACCTGTTTGCGCTGGTGGCAGGCGACGTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACGGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGGTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCGGGAGTATGACCTCGACATc                                    <  2:212609/248‑1 (MQ=255)                                                                                                                                                                                                  gACGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGAtcgtc                        <  1:76385/251‑1 (MQ=255)                                                                                                                                                                                                    cGCCATTGGGTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTcgc                      <  1:235318/251‑1 (MQ=255)                                                                                                                                                                                                           gggTACAGTGGCAGGACCCGTTTCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTcgcg                                                                                                                                                                  <  1:66805/105‑1 (MQ=255)                                                                                                                                                                                                           gggTACAGTGGCAGGACCCGTTTCCGAAAACGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTcgcg                                                                                                                                                                  >  2:66805/1‑105 (MQ=255)                                                                                                                                                                                                            ggTACAGTGGCAGGACCCGGTTCCGTAAACGTGCTACCTGTTTGCGGTGGTGGGAGGCGACTTTGATGTACTGCGCGATAACTTTACCACGCGTTCTGGGCGCGAAGTAGCACTGGAGCTGTAAGTCGATCGCGGCAACCTTGATCGCG‑GCGCGTGGGCGAGGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACAACTATATGTTCGTCGCGGTGGAtt              >  2:205306/1‑251 (MQ=255)                                                                                                                                                                                                                        ggACCCGTTTCCGAATCCGTGCTACCTGTTTGCGCTGGTGGCAAGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCTCGC‑CGTGGGCGATGACCTCGCTTAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCTCGGTGGATTTCTTCAATATgg  <  1:72578/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   CTGGAAGGGCTTTATCAGTCAGGCGATGCGCTTTGCACCCAGTGTGAAGCCGAAGGTTTCCGCCATATTACGTATTATCTCGACCGCCCGGACGTGCTGGCGCGTTTTACCACCAAAATTATTGCCGATAAAATCAAATATCCCTTCCTGCTTTCCAATGGTAACCGCGTTGCGCAAGGCGAACTGGAAAACGGACGCCATTGGGTACAGTGGCAGGACCCGTTCCCGAAACCGTGCTACCTGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGC‑CGTGGGCGATGACCTCGCTGAAAAACTCCATGAAATGGGATGAAGAACGCTTTGGCCTGGAGTATGACCTCGACATCTATATGATCGTCGCGGTGGATTTCTTCAATATGG  >  NC_000913/990937‑991402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑