Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	237,657	C→T	S108F (TCC→TTC)	yafT →	lipoprotein YafT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	237,657	0	C	T	100.0%	63.7 / NA	20	S108F (TCC→TTC)	yafT	lipoprotein YafT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

AAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACT  >  NC_000913/237422‑237898                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  aaaaaaGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTAt                                                                                                                                                                                                                                    <  1:100140/251‑1 (MQ=255)  aaaaaGCTCAAGTCACACAAAGTAATATTAATAACAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATc                                                                                                                                                                                                                                   >  1:222300/1‑251 (MQ=255)                           tatTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACtt                                                                                                                                                                                                          >  1:45058/1‑251 (MQ=255)                            atTAATAAAAACACACCCCAGAAACTGACAGACAAAGATTTATTTGGAAATGAAACCAATCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGATGGATGGAGATGAGTTCCGCGTTCACATTAATTCTCCTGAAATACTTGTTCAATCAGGCAACAGAGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCAAATTCTCTGGTATACAGGACAGGCAAAAGCATCTGACTTg                                                                                                                                                                                                         >  2:59677/1‑251 (MQ=255)                                 taaAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTaac                                                                                                                                                                                                     >  2:245275/1‑250 (MQ=255)                                                        cagacaAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAAc                                                                                                                                                                             <  1:107009/251‑1 (MQ=255)                                                                        cGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTg                                                                                                                                                             <  2:184801/251‑1 (MQ=255)                                                                             aTGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTgag                                                                                                                                                        <  1:56418/251‑1 (MQ=255)                                                                                aaaCCGCTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGGAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAat                                                                                                                                                     <  2:129152/251‑1 (MQ=255)                                                                                                                 aaGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGTAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCt                                                                                                                    <  1:49175/251‑1 (MQ=255)                                                                                                                                                   ccgtAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCAGTATGCAGGAGGAGATGCGAAAATAGGATACTGTTTTCACATTATCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGAGGTTGCCAGTGCTGAGAAGATGAACTGGATGCAGGCACTGCGGTTGGTGGCTGCAAAATGACATCAGAAGGCGATTATTGttt                                                                                     <  2:113696/245‑1 (MQ=255)                                                                                                                                                             ccTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCAGTATGCAGGAGGAGATGCGAAAATATTATACTTTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCATGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATg                                                                          <  1:129581/249‑1 (MQ=255)                                                                                                                                                                     aCTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACGGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAg                                                                <  2:222300/251‑1 (MQ=255)                                                                                                                                                                                           gcACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTTCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGaa                                          <  1:115116/251‑1 (MQ=255)                                                                                                                                                                                           gcACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGaa                                          <  2:45058/251‑1 (MQ=255)                                                                                                                                                                                                      cATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTAt                               >  1:5604/1‑251 (MQ=255)                                                                                                                                                                                                                     gaTGCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATaaa                 >  1:187734/1‑250 (MQ=255)                                                                                                                                                                                                                        gCGAAAATATTATACTGTTTTCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAAtt             >  2:222408/1‑250 (MQ=255)                                                                                                                                                                                                                                attatACTGTTTTCACATTCTCTGGTATCCCGGCCAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGCAATCAACAGTATGGTCCGACTATAAAAATGACAACCTc     >  2:141861/1‑251 (MQ=255)                                                                                                                                                                                                                                   ataCTGTTTTCACATTCTCTGGTATCCCGGACACACAACAGCCTCTGACTTGTAACACAAACAAAGATAAAAATGAGAACGAGGCTGTCGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAACTGACAAACTCACt  >  1:114604/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  AAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTCCGCGTTCCCCTTAATTCTCCTGTAATACTTGTTCAATCCGGCAACCGCGCACCGGAAACCATTATGCAGGAGGAGATGCGAAAATATTATACTGTTTCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACT  >  NC_000913/237422‑237898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑