breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,368 G→A S31F (TCC→TTC)  yaaA ← DNA binding and peroxide stress response protein YaaA
RA 22,796 G→A G136S (GGT→AGT)  ileS → isoleucine‑‑tRNA ligase
RA 25,374 G→A A56A (GCG→GCA lspA → lipoprotein signal peptidase
RA 26,931 G→A V219M (GTG→ATG)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
RA 30,326 G→A E226K (GAA→AAA)  carA → carbamoyl‑phosphate synthetase small subunit
RA 34,521 G→A Q74Q (CAG→CAA caiF → DNA‑binding transcriptional activator CaiF
RA 58,044 G→A K227K (AAG→AAA djlA → co‑chaperone protein DjlA
RA 59,295 G→A intergenic (+16/+392) yabQ → / ← rluA protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
RA 61,932 G→A Q445* (CAG→TAG)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 75,749 G→A H517H (CAC→CAT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 80,413 T→C H151R (CAC→CGC)  leuC ← 3‑isopropylmalate dehydratase subunit LeuC
RA 87,729 G→A A125T (GCA→ACA)  ilvH → acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit
RA 98,436 C→T R12C (CGC→TGC)  ftsW → peptidoglycan glycosyltransferase FtsW
RA 112,666 T→C D185G (GAT→GGT)  coaE ← dephospho‑CoA kinase
RA 115,017 G→A R236R (CGC→CGT hofC ← inner membrane protein HofC
RA 131,517 G→A intergenic (‑257/‑98) yacH ← / → acnB DUF3300 domain‑containing protein YacH/AcnB
RA 134,947 G→A I212I (ATC→ATT speD ← S‑adenosylmethionine decarboxylase proenzyme
RA 144,163 C→T S154S (TCC→TCT yadH → putative ABC transporter membrane subunit YadH
RA 145,144 C→T P22S (CCT→TCT)  yadE → putative polysaccharide deacetylase lipoprotein YadE
RA 157,279 C→T A152T (GCA→ACA)  folK ← 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
RA 159,061 C→T Q22Q (CAG→CAA pcnB ← poly(A) polymerase I
RA 159,971 C→T G48S (GGT→AGT)  gluQ ← glutamyl‑Q tRNA(Asp) synthetase
RA 162,659 C→T R185R (CGC→CGT hrpB → RNA‑dependent NTPase HrpB
RA 171,222 C→T F216F (TTC→TTT fhuD → iron(III) hydroxamate ABC transporter periplasmic binding protein
RA 173,721 C→T D388N (GAC→AAC)  hemL ← glutamate‑1‑semialdehyde 2,1‑aminomutase
RA 181,564 C→T T227T (ACC→ACT degP → periplasmic serine endoprotease DegP
RA 202,488 G→A V130I (GTT→ATT)  fabZ → 3‑hydroxy‑acyl‑[acyl‑carrier‑protein] dehydratase
RA 209,650 A→G intergenic (+70/‑29) accA → / → ldcC acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2
RA 211,411 C→T P578L (CCC→CTC)  ldcC → lysine decarboxylase 2
RA 211,536 C→T P620S (CCA→TCA)  ldcC → lysine decarboxylase 2
RA 213,688 C→T S82N (AGC→AAC)  rof ← modulator of Rho‑dependent transcription termination
RA 214,607 C→T T106I (ACC→ATC)  yaeQ → uncharacterized protein YaeQ
RA 215,947 C→T P227S (CCC→TCC)  nlpE → sensor lipoprotein NlpE
RA 218,136 C→T A214T (GCG→ACG)  proS ← proline‑‑tRNA ligase
RA 222,784 C→T intergenic (‑139/‑49) metN ← / → gmhB L‑methionine/D‑methionine ABC transporter ATP binding subunit/D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase
RA 232,646 C→T G437D (GGC→GAC)  mltD ← membrane‑bound lytic murein transglycosylase D
RA 237,310 A→G intergenic (+303/‑25) aspV → / → yafT tRNA‑Asp/lipoprotein YafT
RA 239,802 C→T E130K (GAA→AAA)  yafV ← 2‑oxoglutaramate amidase
RA 240,993 C→T D771N (GAT→AAT)  fadE ← acyl‑CoA dehydrogenase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
RA 250,724 C→T intergenic (+2590/‑174) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
RA 251,069 C→T P58S (CCG→TCG)  dinB → DNA polymerase IV
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 258,986 C→T pseudogene (311/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
RA 279,397 C→T V95V (GTG→GTA insB2 ← IS1 family protein InsB
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 286,737 G→A G448D (GGC→GAC)  yagG → putative D‑xylonate transporter YagG
RA 286,741 G→A E449E (GAG→GAA yagG → putative D‑xylonate transporter YagG
RA 303,936 C→T E83K (GAA→AAA)  ykgJ ← putative metal‑chelating domain‑containing protein YkgJ
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 359,642 G→A A52T (GCG→ACG)  cynS → cyanase
RA 377,292 C→T P174L (CCT→CTT)  yaiL → DUF2058 domain‑containing protein YaiL
RA 379,592 G→A intergenic (‑21/+14) frmA ← / ← frmR S‑(hydroxymethyl)glutathione dehydrogenase/DNA‑binding transcriptional repressor FrmR
RA 393,962 C→T pseudogene (993/1449 nt) yaiT → putative autotransporter YaiT
RA 402,101 G→A D119N (GAC→AAC)  phoA → alkaline phosphatase
RA 424,981 C→T intergenic (‑63/‑30) acpH ← / → queA acyl carrier protein phosphodiesterase/tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
RA 466,884 G→A A143A (GCC→GCT ybaE ← uncharacterized protein YbaE
RA 469,175 G→A R102Q (CGG→CAG)  mdlA → ABC transporter family protein MdlA
RA 472,100 C→T R489C (CGC→TGC)  mdlB → ABC transporter family protein MdlB
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 502,527 C→T V238I (GTT→ATT)  ybaL ← putative transporter YbaL
RA 538,869 G→T G413C (GGC→TGC)  ybbW → putative allantoin transporter
RA 548,951 C→T R199C (CGT→TGT)  ylbE → DUF1116 domain‑containing protein YlbE
RA 556,653 G→A intergenic (‑100/+8) ybcI ← / ← ybcJ PF04307 family inner membrane protein YbcI/putative RNA‑binding protein YbcJ
RA 561,489 C→T T598I (ACC→ATC)  sfmD → putative fimbrial usher protein SfmD
RA 562,281 G→A C862Y (TGC→TAC)  sfmD → putative fimbrial usher protein SfmD
RA 573,977 C→T L8F (CTT→TTT)  quuD → prophage antitermination protein Q homolog QuuD
RA 590,965 C→T L738L (CTG→CTA nfrB ← cyclic di‑3',5'‑guanylate‑activated glycosyltransferase
RA 599,895 T→A G394G (GGT→GGA cusA → copper/silver export system RND permease
RA 625,184 G→A Q100Q (CAG→CAA entC → isochorismate synthase EntC
RA 625,239 G→A E119K (GAA→AAA)  entC → isochorismate synthase EntC
RA 626,003 G→A E373E (GAG→GAA entC → isochorismate synthase EntC
RA 632,017 (G)7→8 intergenic (+18/‑165) cstA → / → ybdD pyruvate transporter CstA/PF04328 family protein YbdD
RA 640,875 G→A A375T (GCG→ACG)  ahpF → alkyl hydroperoxide reductase, AhpF component
RA 642,847 G→A G254S (GGT→AGT)  ybdR → putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
RA 644,936 G→A A23V (GCC→GTC)  rna ← RNase I
RA 646,765 G→A Q249* (CAA→TAA)  citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 659,597 G→A P207L (CCT→CTT)  lipA ← lipoyl synthase
RA 663,757 G→A R69R (CGC→CGT dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 672,861 G→A T641T (ACC→ACT leuS ← leucine‑‑tRNA ligase
RA 681,334 G→A E375K (GAG→AAG)  djlC → co‑chaperone DjlC
RA 685,247 G→A T3T (ACC→ACT gltL ← glutamate/aspartate ABC transporter ATP binding subunit
RA 686,770 C→T noncoding (74/163 nt) sroC ← small regulatory RNA SroC
RA 687,474 G→A P92S (CCG→TCG)  gltI ← glutamate/aspartate ABC transporter periplasmic binding protein
RA 690,924 C→T Q287Q (CAG→CAA ybeX ← CorC‑HlyC family protein YbeX
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 694,826 G→A L44L (CTG→TTG)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 702,286 C→T V156I (GTC→ATC)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 703,511 G→A P34L (CCG→CTG)  nagB ← glucosamine‑6‑phosphate deaminase
RA 705,141 G→A V400I (GTT→ATT)  nagE → N‑acetylglucosamine‑specific PTS enzyme IIABC component
RA 709,856 C→T P23S (CCA→TCA)  chiQ → lipoprotein ChiQ
RA 711,895 C→T A2T (GCC→ACC)  ybfE ← ribbon‑helix‑helix domain‑containing protein YbfE
RA 720,064 C→T G133S (GGC→AGC)  speF ← inducible ornithine decarboxylase
RA 721,402 C→T R111H (CGT→CAT)  kdpE ← DNA‑binding transcriptional activator KdpE
RA 722,075 C→T R780R (CGG→CGA kdpD ← sensor histidine kinase KdpD
RA 722,941 C→T G492R (GGG→AGG)  kdpD ← sensor histidine kinase KdpD
RA 737,835 G→A intergenic (+1136/‑257) ybfQ → / → ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
RA 745,696 G→A G178S (GGC→AGC)  pxpA → 5‑oxoprolinase component A
RA 778,688 G→A V317M (GTG→ATG)  tolB → Tol‑Pal system periplasmic protein TolB
RA 779,297 G→A K77K (AAG→AAA pal → peptidoglycan‑associated outer membrane lipoprotein Pal
RA 781,285 G→A intergenic (+63/‑84) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 799,545 G→A G320G (GGG→GGA pgl → 6‑phosphogluconolactonase
RA 805,637 G→A G712D (GGT→GAT)  ybhJ → putative hydratase YbhJ
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 813,709 G→A V62I (GTA→ATA)  uvrB → UvrABC excision nuclease subunit B
RA 826,365 G→A A296A (GCC→GCT ybhS ← ABC exporter membrane subunit YbhS
RA 846,231 T→C E78G (GAA→GGA)  glnQ ← L‑glutamine ABC transporter ATP binding subunit
RA 852,953 G→A noncoding (112/340 nt)
P15P (CCC→CCT
rybA ←
mntS ←
small RNA RybA
small protein MntS
RA 861,095 C→T W504* (TGG→TGA ybiW ← putative pyruvate formate lyase
RA 871,791 G→A Q275Q (CAG→CAA gsiC → glutathione ABC transporter membrane subunit GsiC
RA 884,123 C→T L151L (CTG→TTG)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 921,808 C→T Q488* (CAA→TAA)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 941,667 C→T L237L (CTG→TTG)  dmsA → dimethyl sulfoxide reductase subunit A
RA 949,385 C→T A62T (GCG→ACG)  ycaN ← putative DNA‑binding transcriptional regulator YcaN
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,019,715 G→T D113E (GAC→GAA ompA ← outer membrane protein A
RA 1,033,251 G→A Q371Q (CAG→CAA hyaA → hydrogenase 1 small subunit
RA 1,035,375 G→A G104S (GGC→AGC)  hyaC → hydrogenase 1 cytochrome b subunit
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,115,929 C→T Q218Q (CAG→CAA lpxL ← lauroyl acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,173,479 G→A Y162Y (TAC→TAT mfd ← transcription‑repair coupling factor
RA 1,179,499 G→A D303N (GAT→AAT)  nagK → N‑acetyl‑D‑glucosamine kinase
RA 1,180,740 A→G A176A (GCT→GCC ycfZ ← putative inner membrane protein YcfZ
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,193,169 C→T G202D (GGC→GAC)  mnmA ← tRNA‑specific 2‑thiouridylase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,219,718 G→A intergenic (+517/+2587) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,244,207 G→A T107T (ACC→ACT ycgR ← flagellar brake protein YcgR
RA 1,246,166 G→A A404V (GCC→GTC)  treA ← periplasmic trehalase
RA 1,248,115 G→A L334F (CTC→TTC)  dhaM ← dihydroxyacetone kinase subunit M
RA 1,253,880 G→A D691D (GAC→GAT ycgV ← putative autotransporter adhesin YcgV
RA 1,258,716 G→A intergenic (‑203/‑75) pth ← / → ychH peptidyl‑tRNA hydrolase/stress‑induced protein
RA 1,262,852 G→A A9V (GCA→GTA)  ispE ← 4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase
RA 1,263,854 G→A L47L (CTG→CTA hemA → glutamyl‑tRNA reductase
RA 1,269,279 G→A intergenic (‑4/‑44) ldrA ← / → rdlA small toxic polypeptide LdrA/putative antisense regulatory RNA RdlA
RA 1,275,732 G→A T33I (ACC→ATC)  narL ← DNA‑binding transcriptional dual regulator NarL
RA 1,279,166 G→A A404T (GCG→ACG)  narK → nitrate:nitrite antiporter NarK
RA 1,284,154 G→A C184Y (TGC→TAC)  narH → nitrate reductase A subunit beta
RA 1,297,195 G→A G309G (GGC→GGT adhE ← fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,305,512 G→A A253A (GCG→GCA oppD → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,313,378 G→A L96F (CTC→TTC)  yciC ← putative inner membrane protein
RA 1,314,016 G→A intergenic (‑353/‑4) yciC ← / → ompW putative inner membrane protein/outer membrane protein W
RA 1,315,078 G→A T49T (ACC→ACT yciE ← DUF892 domain‑containing protein YciE
RA 1,315,678 G→A L31L (CTC→CTT yciF ← DUF892 domain‑containing protein YciF
RA 1,321,850 A→G V366A (GTT→GCT)  trpE ← anthranilate synthase subunit TrpE
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,339,286 G→A intergenic (‑126/‑44) ribA ← / → pgpB GTP cyclohydrolase 2/phosphatidylglycerophosphatase B
RA 1,340,566 G→A E3E (GAG→GAA lapB → lipopolysaccharide assembly protein B
RA 1,341,553 G→A G332G (GGG→GGA lapB → lipopolysaccharide assembly protein B
RA 1,345,043 G→A S567F (TCT→TTT)  pdeR ← cyclic di‑GMP phosphodiesterase PdeR
RA 1,345,822 G→A D307D (GAC→GAT pdeR ← cyclic di‑GMP phosphodiesterase PdeR
RA 1,345,878 C→T G289S (GGT→AGT)  pdeR ← cyclic di‑GMP phosphodiesterase PdeR
RA 1,347,874 G→A R347C (CGC→TGC)  rnb ← RNase II
RA 1,349,363 G→A L249F (CTT→TTT)  yciW ← putative peroxidase YciW
RA 1,350,035 C→T V25I (GTT→ATT)  yciW ← putative peroxidase YciW
RA 1,350,046 G→A T21M (ACG→ATG)  yciW ← putative peroxidase YciW
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,359,826 G→A G361G (GGC→GGT puuA ← glutamate‑putrescine ligase
RA 1,363,995 C→T A418V (GCC→GTC)  puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,375,364 G→A W134* (TGG→TGA ycjQ → D‑guloside 3‑dehydrogenase
RA 1,376,943 G→A V38M (GTG→ATG)  ycjS → D‑glucoside 3‑dehydrogenase
RA 1,378,748 G→A G289S (GGC→AGC)  ycjT → kojibiose phosphorylase
RA 1,378,754 G→A A291T (GCG→ACG)  ycjT → kojibiose phosphorylase
RA 1,379,153 G→A V424I (GTT→ATT)  ycjT → kojibiose phosphorylase
RA 1,384,743 C→T H209H (CAC→CAT ycjX → DUF463 domain‑containing protein YcjX
RA 1,388,891 C→T intergenic (‑80/‑39) tpx ← / → ycjG lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase
RA 1,390,303 C→T R99K (AGA→AAA)  mpaA ← murein tripeptide amidase A
RA 1,390,834 C→T G10D (GGC→GAC)  ymjC ← putative uncharacterized protein YmjC
RA 1,396,062 C→T intergenic (‑140/‑50) ynaI ← / → insH4 small conductance mechanosensitive channel YnaI/IS5 family transposase and trans‑activator
RA 1,401,254 C→T V174I (GTA→ATA)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,411,942 C→T E3K (GAA→AAA)  ttcA ← tRNA cytosine(32) 2‑sulfurtransferase TtcA
RA 1,415,168 C→T G740E (GGA→GAA)  recE ← exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
RA 1,416,515 C→T R291H (CGC→CAC)  recE ← exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
RA 1,418,843 C→T A58V (GCC→GTC)  sieB → phage superinfection exclusion protein
RA 1,428,218 C→T G170D (GGC→GAC)  insH5 ← IS5 family transposase and trans‑activator
RA 1,430,016 C→T A323V (GCC→GTC)  stfR → putative prophage side tail fiber protein StfR
RA 1,435,735 C→T intergenic (‑116/+25) uspF ← / ← ompN universal stress protein F/outer membrane porin N
RA 1,436,556 C→T G113E (GGA→GAA)  ompN ← outer membrane porin N
RA 1,440,002 C→T G261G (GGG→GGA ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,444,525 C→T P492L (CCC→CTC)  ydbH → putative phospholipid transport factor YdbH
RA 1,444,631 C→T T527T (ACC→ACT ydbH → putative phospholipid transport factor YdbH
RA 1,461,156 C→T R88R (CGC→CGT paaJ → beta‑ketoadipyl‑CoA thiolase
RA 1,461,753 C→T T287T (ACC→ACT paaJ → beta‑ketoadipyl‑CoA thiolase
RA 1,467,929 C→T E300K (GAA→AAA)  insD2 ← IS2 insertion element protein InsB
RA 1,488,147 C→T intergenic (+112/‑85) ydcF → / → aldA DUF218 domain‑containing protein YdcF/aldehyde dehydrogenase A
RA 1,489,861 C→T intergenic (+190/‑1041) aldA → / → cybB aldehyde dehydrogenase A/superoxide oxidase
RA 1,494,852 (A)5→6 coding (220/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,495,978 G→A D231N (GAT→AAT)  ydcJ → DUF1338 domain‑containing protein YdcJ
RA 1,509,592 G→A G29S (GGC→AGC)  hicB → antitoxin of the HicA‑HicB toxin‑antitoxin system/ DNA‑binding transcriptional repressor HicB
RA 1,509,772 T→A S89T (TCA→ACA)  hicB → antitoxin of the HicA‑HicB toxin‑antitoxin system/ DNA‑binding transcriptional repressor HicB
RA 1,510,883 C→T P294L (CCT→CTT)  ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,514,317 G→A E163K (GAA→AAA)  ydcU → putative ABC transporter membrane subunit YdcU
RA 1,521,013 G→A P685S (CCC→TCC)  yncD ← putative TonB‑dependent outer membrane receptor
RA 1,522,601 G→A T155T (ACC→ACT yncD ← putative TonB‑dependent outer membrane receptor
RA 1,523,628 A→G T108A (ACC→GCC)  yncE → PQQ‑like domain‑containing protein YncE
RA 1,531,969 G→A E52K (GAA→AAA)  ydcC → H repeat‑associated putative transposase YdcC
RA 1,540,097 C→T A832T (GCA→ACA)  narZ ← nitrate reductase Z subunit alpha
RA 1,544,540 C→T intergenic (‑480/+218) narU ← / ← yddK nitrate/nitrite transporter NarU/leucine‑rich repeat domain‑containing protein YddK
RA 1,555,122 C→T G183D (GGC→GAC)  maeA ← malate dehydrogenase (oxaloacetate‑decarboxylating)
RA 1,557,720 G→A L107F (CTC→TTC)  ddpF ← putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
RA 1,558,635 C→T R128Q (CGG→CAG)  ddpD ← putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD
RA 1,559,106 C→T A269T (GCA→ACA)  ddpC ← putative D,D‑dipeptide ABC transporter membrane subunit DdpC
RA 1,567,576 G→A F416F (TTC→TTT digH ← divisome localized glycosyl hydrolase
RA 1,569,391 A→G F367L (TTC→CTC)  gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,573,933 G→A P424S (CCG→TCG)  pqqL ← periplasmic metalloprotease
RA 1,582,470 C→T A19T (GCA→ACA)  ydeN ← putative sulfatase YdeN
RA 1,593,789 C→T intergenic (‑1347/+4828) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,594,311 C→T intergenic (‑1869/+4306) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,595,917 C→T intergenic (‑3475/+2700) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,597,327 C→T intergenic (‑4885/+1290) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,597,608 C→T intergenic (‑5166/+1009) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,598,395 C→T intergenic (‑5953/+222) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,599,945 C→T A89T (GCA→ACA)  lsrK ← autoinducer‑2 kinase
RA 1,600,113 C→T G33S (GGC→AGC)  lsrK ← autoinducer‑2 kinase
RA 1,603,061 C→T L15L (CTG→TTG)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,603,368 C→T A117V (GCC→GTC)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,617,801 C→T G258G (GGC→GGT ydeA → L‑arabinose exporter
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,647,571 T→C T22T (ACA→ACG ydfX ← protein YdfX
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,695,623 G→A T150I (ACC→ATC)  uidA ← beta‑glucuronidase
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,737,533 G→A L5F (CTT→TTT)  cydH ← cytochrome bd‑I accessory subunit CydH
RA 1,741,751 G→A W113* (TGG→TGA cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,752,136 G→A T565T (ACC→ACT ydhV ← putative oxidoreductase YdhV
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,843,127 G→A E253K (GAA→AAA)  gdhA → glutamate dehydrogenase
RA 1,875,677 C→T T2I (ACC→ATC)  nimT → 2‑nitroimidazole exporter
RA 1,876,063 C→T P131S (CCT→TCT)  nimT → 2‑nitroimidazole exporter
RA 1,889,453 C→T G98G (GGG→GGA fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,893,564 C→T F66F (TTC→TTT yoaB → RutC family protein YoaB
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,895,353 C→T D183D (GAC→GAT pabB → aminodeoxychorismate synthase subunit 1
RA 2,004,071 C→T A67V (GCC→GTC)  fliD → flagellar filament capping protein
RA 2,029,236 T→C N44S (AAT→AGT)  yedI ← DUF808 domain‑containing inner membrane protein YedI
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,050,238 T→C S1767S (AGT→AGC yeeJ → inverse autotransporter adhesin
RA 2,056,599 T→C intergenic (+84/‑259) amn → / → yeeN AMP nucleosidase/putative transcriptional regulator YeeN
RA 2,100,672 T→C E199E (GAA→GAG gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,269,856 C→T intergenic (+289/‑123) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,316,202 C→T A9V (GCC→GTC)  rcsB → DNA‑binding transcriptional activator RcsB
JC JC 2,382,523 IS1 (–) +9 bp [rbn] [rbn]
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,448,152 C→T A97T (GCG→ACG)  prmB ← ribosomal protein L3 N(5)‑glutamine methyltransferase
RA 2,464,335 C→T A225T (GCT→ACT)  mlaA ← intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
RA 2,472,282 C→T G36R (GGA→AGA)  yfdM ← putative methyltransferase YfdM
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,491,112 C→T R280Q (CGA→CAA)  oxc ← oxalyl‑CoA decarboxylase
RA 2,497,429 C→T L289L (CTG→CTA alaC ← glutamate‑‑pyruvate aminotransferase AlaC
RA 2,509,277 T→C E50E (GAA→GAG glk ← glucokinase
RA 2,547,929 C→T G419G (GGC→GGT murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
RA 2,556,466 C→T D278N (GAT→AAT)  eutC ← ethanolamine ammonia‑lyase subunit beta
RA 2,561,146 C→T intergenic (+248/‑222) yffL → / → yffM uncharacterized protein YffL/uncharacterized protein YffM
RA 2,574,537 C→T S156N (AGC→AAC)  eutQ ← putative ethanolamine utilization acetate kinase EutQ
RA 2,631,667 G→A A290V (GCA→GTA)  guaA ← GMP synthetase
RA 2,675,335 G→A L143F (CTC→TTC)  yphC ← putative zinc‑binding dehydrogenase YphC
RA 2,764,953 C→T W67* (TGG→TGA abpA ← anti‑bacteriophage protein
RA 2,767,045 G→A G21D (GGC→GAC)  rnlB → antitoxin RnlB
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 3,055,907 G→A R99H (CGC→CAC)  zapA → cell division protein ZapA
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,143,279 G→A L296F (CTC→TTC)  hybB ← hydrogenase 2 membrane subunit
RA 3,195,117 G→A noncoding (124/144 nt) sibE ← small RNA SibE
RA 3,195,432 G→A A441V (GCC→GTC)  hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
RA 3,202,387 C→T A166V (GCG→GTG)  cca → fused CCA tRNA nucleotidyltransferase/phosphohydrolase
RA 3,205,054 G→A D121N (GAT→AAT)  plsY → putative glycerol‑3‑phosphate acyltransferase
RA 3,259,756 G→A L119L (CTG→TTG)  tdcF ← putative enamine/imine deaminase
RA 3,262,529 G→A P378S (CCG→TCG)  tdcD ← propionate kinase
RA 3,265,043 G→A A329V (GCT→GTT)  tdcB ← catabolic threonine dehydratase
RA 3,267,794 G→A intergenic (+196/‑60) tdcR → / → yhaB DNA‑binding transcriptional activator TdcR/protein YhaB
RA 3,289,167 G→A V118V (GTG→GTA yraJ → putative fimbrial usher protein YraJ
RA 3,291,761 G→A A141T (GCT→ACT)  yraK → putative fimbrial adhesin YraK
RA 3,311,967 G→A R271C (CGT→TGT)  truB ← tRNA pseudouridine(55) synthase
RA 3,314,130 G→A L629F (CTC→TTC) 
L629F (CTC→TTC) 
L629F (CTC→TTC) 
infB ←
infB ←
infB ←
translation initiation factor IF‑2beta'
translation initiation factor IF‑2beta'
translation initiation factor IF‑2beta'
RA 3,324,879 G→A D11D (GAC→GAT folP ← dihydropteroate synthase
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,337,832 G→A T20T (ACC→ACT mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,339,982 G→A S28S (TCC→TCT mlaF ← intermembrane phospholipid transport system, ATP binding subunit MlaF
RA 3,343,250 G→A V139M (GTG→ATG)  lptC → lipopolysaccharide transport system protein LptC
RA 3,346,302 G→A E44K (GAG→AAG)  hpf → ribosome hibernation‑promoting factor
RA 3,347,092 G→A intergenic (+23/‑23) ptsN → / → rapZ phosphotransferase system enzyme IIA(Ntr)/RNase adaptor protein RapZ
RA 3,347,370 G→A D86N (GAT→AAT)  rapZ → RNase adaptor protein RapZ
RA 3,355,138 G→A E138E (GAG→GAA gltB → glutamate synthase subunit GltB
RA 3,359,010 G→A G1429D (GGT→GAT)  gltB → glutamate synthase subunit GltB
RA 3,359,916 G→A G240E (GGG→GAG)  gltD → glutamate synthase subunit GltD
RA 3,359,937 G→A G247D (GGC→GAC)  gltD → glutamate synthase subunit GltD
RA 3,377,162 G→A P87S (CCA→TCA)  sspA ← stringent starvation protein A
RA 3,379,594 G→A A135V (GCC→GTC)  zapE ← cell division protein ZapE
RA 3,381,442 G→A A234T (GCG→ACG)  degQ → periplasmic serine endoprotease
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,390,213 G→A A232T (GCG→ACG)  aaeR → DNA‑binding transcriptional activator AaeR
RA 3,399,260 G→A S240F (TCC→TTC)  mreC ← cell shape determining protein MreC
RA 3,420,842 G→A A237T (GCA→ACA)  yhdX → putative ABC transporter membrane subunit YhdX
RA 3,424,524 G→A noncoding (2260/2904 nt) rrlD ← 23S ribosomal RNA
RA 3,428,727 G→A noncoding (36/1542 nt) rrsD ← 16S ribosomal RNA
RA 3,428,825 G→A intergenic (‑63/‑411) rrsD ← / → yrdA 16S ribosomal RNA/hexapeptide repeat‑containing protein YrdA
RA 3,430,588 G→A A84V (GCT→GTT)  aroE ← shikimate dehydrogenase
RA 3,431,506 G→A P153S (CCG→TCG)  yrdD ← putative DNA topoisomerase
RA 3,436,419 G→A E405K (GAG→AAG)  rsmB → 16S rRNA m(5)C967 methyltransferase
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,439,003 C→T S43N (AGC→AAC)  zntR ← DNA‑binding transcriptional activator ZntR
RA 3,450,966 G→A R133R (CGC→CGT rplB ← 50S ribosomal subunit protein L2
RA 3,466,533 G→A L65L (CTG→TTG)  bfr ← bacterioferritin
RA 3,476,485 G→A intergenic (‑45/+122) yheO ← / ← fkpA DNA‑binding transcriptional regulator YheO/peptidyl‑prolyl cis‑trans isomerase FkpA
RA 3,477,781 C→T L48L (CTG→TTG)  slyX → protein SlyX
RA 3,479,373 C→T G412D (GGT→GAT)  kefB ← K(+) : H(+) antiporter KefB
RA 3,480,842 C→T R107H (CGT→CAT)  kefG ← glutathione‑regulated potassium‑efflux system ancillary protein KefG
RA 3,487,784 C→T L332L (CTG→TTG)  yhfK → putative transporter YhfK
RA 3,491,210 C→T G85D (GGT→GAT)  fic ← putative adenosine monophosphate‑‑protein transferase Fic
RA 3,500,982 C→T T358I (ACC→ATC)  frlA → fructoselysine/psicoselysine transporter
RA 3,505,751 C→T G90G (GGG→GGA yhfS ← putative aminotransferase YhfS
RA 3,509,233 C→T R194H (CGC→CAC)  yhfW ← putative mutase YhfW
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,667,023 C→T Q186Q (CAG→CAA gadA ← glutamate decarboxylase A
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,705,875 G→A intergenic (‑85/+223) dppB ← / ← dppA dipeptide ABC transporter membrane subunit DppB/dipeptide ABC transporter periplasmic binding protein
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
MC JC 3,708,467 Δ168 bp [proK] [proK]
RA 3,720,801 C→T T41I (ACA→ATA)  insJ → insertion element IS150 protein InsA
RA 3,725,152 G→A A56V (GCT→GTT)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,746,493 G→A G134S (GGT→AGT)  yiaO → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein
RA 3,747,484 A→T H134L (CAT→CTT)  lyxK → L‑xylulose kinase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,780,988 G→A A388T (GCT→ACT)  lldD → L‑lactate dehydrogenase
RA 3,785,167 G→A intergenic (‑152/‑93) yibN ← / → gpmM putative sulfurtransferase YibN/2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
RA 3,787,826 G→A G330D (GGT→GAT)  envC → murein hydrolase activator EnvC
RA 3,792,096 G→A R152R (CGC→CGT kbl ← 2‑amino‑3‑ketobutyrate CoA ligase
RA 3,806,801 C→T K89K (AAG→AAA waaG ← lipopolysaccharide glucosyltransferase I
RA 3,811,123 G→A S10S (AGC→AGT mutM ← DNA‑formamidopyrimidine glycosylase
RA 3,815,416 C→T G118E (GGA→GAA)  pyrE ← orotate phosphoribosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,555 C→T intergenic (+16/‑205) yicC → / → dinD putative RNase adaptor protein YicC/DNA damage‑inducible protein D
RA 3,818,527 C→T T256T (ACC→ACT dinD → DNA damage‑inducible protein D
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,829,611 G→A A223T (GCC→ACC)  xanP → xanthine:H(+) symporter XanP
RA 3,833,353 G→A C395C (TGC→TGT yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,839,315 G→A P227S (CCG→TCG)  nlpA ← lipoprotein‑28
RA 3,839,410 G→A P195L (CCC→CTC)  nlpA ← lipoprotein‑28
RA 3,843,428 C→T G121D (GGC→GAC)  adeQ ← adenine transporter
RA 3,844,489 G→A D176N (GAC→AAC)  adeD → adenine deaminase
RA 3,853,196 G→A noncoding (62/140 nt) istR ← small RNA IstR‑2
RA 3,867,864 G→A G46D (GGC→GAC)  yidQ → DUF1375 domain‑containing putative lipoprotein YidQ
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,870,078 G→A M234I (ATG→ATA cbrA → colicin M resistance protein
RA 3,872,137 G→A L288F (CTC→TTC)  dgoD ← D‑galactonate dehydratase
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,889,459 G→A E244K (GAA→AAA)  tnaA → tryptophanase
RA 3,890,923 G→A V230M (GTG→ATG)  tnaB → tryptophan:H(+) symporter TnaB
RA 3,894,347 G→A G156D (GGT→GAT)  yieE → putative phosphopantetheinyl transferase
RA 3,903,658 G→A A15V (GCT→GTT)  bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,910,444 C→T intergenic (‑46/+41) pstC ← / ← pstS phosphate ABC transporter membrane subunit PstC/phosphate ABC transporter periplasmic binding protein
RA 3,911,379 C→T Q49Q (CAG→CAA pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,914,907 G→A D98D (GAC→GAT glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA 3,916,100 G→A R426C (CGT→TGT)  atpD ← ATP synthase F1 complex subunit beta
RA 3,917,093 G→A P95S (CCG→TCG)  atpD ← ATP synthase F1 complex subunit beta
RA 3,920,136 G→A P90S (CCG→TCG)  atpH ← ATP synthase F1 complex subunit delta
RA 3,954,459 C→T R328C (CGT→TGT)  ilvD → dihydroxy‑acid dehydratase
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,997,261 G→A V27M (GTG→ATG)  yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 4,005,738 G→A intergenic (+7/‑126) pldA → / → recQ outer membrane phospholipase A/ATP‑dependent DNA helicase RecQ
RA 4,009,250 G→A W27* (TGG→TGA pldB → lysophospholipase L2
RA 4,009,552 G→A G128D (GGT→GAT)  pldB → lysophospholipase L2
RA 4,015,364 T→C K269R (AAG→AGG)  ysgA ← putative dienelactone hydrolase
RA 4,017,606 G→A A92T (GCC→ACC)  rmuC → putative recombination limiting protein RmuC
RA 4,020,530 G→A G102D (GGC→GAC)  ubiB → ubiquinone biosynthesis protein UbiB
RA 4,022,421 G→A K68K (AAG→AAA tatB → twin arginine protein translocation system ‑ TatB protein
RA 4,025,213 G→A G76S (GGC→AGC)  ubiD → 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
RA 4,027,752 G→A L341L (CTG→TTG)  fadA ← 3‑ketoacyl‑CoA thiolase
RA 4,031,591 G→A S144N (AGC→AAC)  pepQ → Xaa‑Pro dipeptidase
RA 4,033,621 G→A Q159Q (CAG→CAA trkH → K(+) transporter TrkH
RA 4,037,278 G→A noncoding (19/76 nt) alaT → tRNA‑Ala
RA 4,064,842 C→T V309M (GTG→ATG)  yihO ← putative sulfoquinovose transporter
RA 4,069,091 C→T Q62Q (CAG→CAA yihQ ← sulfoquinovosidase
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,090,609 A→G I106T (ATC→ACC)  frvX ← peptidase M42 family protein FrvX
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,111,379 C→T P43P (CCG→CCA tpiA ← triose‑phosphate isomerase
RA 4,112,203 C→T G4G (GGG→GGA yiiQ ← DUF1454 domain‑containing protein YiiQ
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,193,850 G→A L119L (CTG→TTG)  thiE ← thiamine phosphate synthase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,238,016 G→A R128Q (CGG→CAG)  yjbH → putative lipoprotein YjbH
RA 4,256,705 C→T R23R (CGC→CGT dgkA → diacylglycerol kinase
RA 4,256,736 C→T Q34* (CAG→TAG)  dgkA → diacylglycerol kinase
RA 4,257,546 C→T V144V (GTC→GTT lexA → DNA‑binding transcriptional repressor LexA
RA 4,269,737 C→T G108G (GGC→GGT aphA → acid phosphatase/phosphotransferase
RA 4,269,904 C→T P164L (CCG→CTG)  aphA → acid phosphatase/phosphotransferase
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,385,260 C→T V474V (GTC→GTT yjeM → putative transporter YjeM
RA 4,386,666 C→T W902* (TGG→TAG)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,389,369 A→G M1A (GTG→GCG) † mscM ← miniconductance mechanosensitive channel MscM
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,442,405 C→T C8C (TGC→TGT tamA → translocation and assembly module subunit TamA
RA 4,453,213 G→A A215T (GCA→ACA)  ytfT → galactofuranose ABC transporter putative membrane subunit YtfT
RA 4,456,339 C→T N185N (AAC→AAT mpl → UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
RA 4,457,569 C→T Q99Q (CAG→CAA yjgA ← putative ribosome biogenesis factor YjgA
RA 4,457,803 G→A D21D (GAC→GAT yjgA ← putative ribosome biogenesis factor YjgA
RA 4,457,874 C→T intergenic (‑9/‑85) yjgA ← / → tldE putative ribosome biogenesis factor YjgA/metalloprotease subunit TldE
RA 4,460,754 C→T G636D (GGC→GAC)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,461,891 C→T G257D (GGC→GAC)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,462,277 C→T Q128Q (CAG→CAA nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,462,776 C→T intergenic (‑116/+278) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,462,941 C→T intergenic (‑281/+113) nrdD ← / ← treC anaerobic ribonucleoside‑triphosphate reductase/trehalose‑6‑phosphate hydrolase
RA 4,463,138 C→T V524V (GTG→GTA treC ← trehalose‑6‑phosphate hydrolase
RA 4,471,404 C→T G15D (GGC→GAC)  pyrI ← aspartate carbamoyltransferase, PyrI subunit
RA 4,486,407 C→T L64L (CTG→TTG)  lptF → lipopolysaccharide transport system protein LptF
RA 4,494,432 C→T intergenic (‑26/‑191) idnD ← / → idnK L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
RA 4,495,621 C→T A197T (GCC→ACC)  ahr ← NADPH‑dependent aldehyde reductase Ahr
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,512,344 C→T G24E (GGA→GAA)  fecD ← ferric citrate ABC transporter membrane subunit FecD
RA 4,513,529 C→T K260K (AAG→AAA fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,517,591 C→T D43N (GAT→AAT)  fecR ← ferric citrate regulator FecR
RA 4,518,930 T→C intergenic (+128/+408) insA7 → / ← yjhU IS1 family repressor protein InsA/putative DNA‑binding transcriptional regulator YjhU
RA 4,521,584 C→T G146D (GGT→GAT)  yjhF ← putative transporter YjhF
RA 4,523,866 C→T D77N (GAC→AAC)  yjhG ← D‑xylonate dehydratase
RA 4,526,856 C→T Q11Q (CAG→CAA sgcR ← putative DNA‑binding transcriptional regulator SgcR
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,549,907 C→T intergenic (+197/+46) fimH → / ← gntP type 1 fimbriae D‑mannose specific adhesin/fructuronate transporter
RA 4,618,086 G→A S255N (AGC→AAC)  deoC → deoxyribose‑phosphate aldolase
RA 4,618,623 G→A G132D (GGC→GAC)  deoA → thymidine phosphorylase
RA 4,631,521 G→A M263I (ATG→ATA slt → soluble lytic murein transglycosylase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD
RA 4,638,495 G→A G106G (GGG→GGA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 730332–730565 731641–731560 996–1310 32 [29] [29] 36 rhsC rhs element protein RhsC
* * ÷ NC_000913 1196320 1211460 15141 32 [31] [31] 37 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978504 10–776 100 [0] [0] 133 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423871–3424232 3424455–3424239 8–585 32 [31] [31] 32 [rrlD] [rrlD]
* * ÷ NC_000913 4037971–4038685 4039209–4038723 39–1239 32 [31] [31] 32 rrlA 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 78062996 (1.010)23 (0.330) 16/438 2.9 26.9% noncoding (76/76 nt) lysT tRNA‑Lys
?NC_000913 780919 = 54 (0.770)intergenic (+1/‑149) lysW/valZ tRNA‑Lys/tRNA‑Val
* ? NC_000913 = 1397238NA (NA)99 (1.040) 59/590 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)133 (1.400) 81/590 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL