Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,344	C→T	G24E (GGA→GAA)	fecD ←	ferric citrate ABC transporter membrane subunit FecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,344	0	C	T	100.0%	216.6 / NA	63	G24E (GGA→GAA)	fecD	ferric citrate ABC transporter membrane subunit FecD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/28);  total (35/28)

ATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGC  >  NC_000913/4512056‑4512609                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                          aTGCCGCCCGCAAATGCCAGCAGCGGCAACACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTTTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAg                                                                                                                                                                                                                                                                 <  2:66856/299‑1 (MQ=255)          gcAAAGGCCAGCAGCGGCATCACCATCACGTGCAGTGACGGCATAAGAAGTAGATCCCCCACAGAGGCCAGGCTTGCTGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAAc                                                                                                                                                                                                                                                          <  2:405674/297‑1 (MQ=255)          gcAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCNGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAg                                                                                                                                                                                                                                                        <  1:556860/299‑1 (MQ=255)          gcAAAGGCCAGCAGCGGCAACACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCAAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAAc                                                                                                                                                                                                                                                          <  1:709620/297‑1 (MQ=255)            aaaGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAAc                                                                                                                                                                                                                                                          <  2:503606/295‑1 (MQ=255)               ggCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCAAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGATCGTGTCCGGCCTGCCAGTCGGTAAGCAGCGCACGCCCAGGCACGGGGATCACTTCCatat                                                                                                                                                                                                                                                                     >  2:741766/1‑281 (MQ=255)                                  aTCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAggg                                                                                                                                                                                                                                 <  1:278711/298‑1 (MQ=255)                                        ggCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCATCATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGCCGGTACTCCATCAATACATGATCATGCTCGTGCCCGACCTGCCAGTCAGTCGGCAGCGCGCGCCACGGCACGGGGATCCCTTCCATATGGAGTGATAACAGCACACAACCCGACAGGGCACGGGTTATGaaa                                                                                                                                                                                                                         >  2:301690/1‑300 (MQ=255)                                                      aagaagTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAAt                                                                                                                                                                                                           >  1:715575/1‑300 (MQ=255)                                                                 cccccACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCACTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACATCCCGCCAGGGCAAGAGTGATGAAAATAACCAGCGCAATTTTCATCCTc                                                                                                                                                                                                 >  2:490362/1‑299 (MQ=255)                                                                                ggCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCa                                                                                                                                                                                 >  1:527257/1‑300 (MQ=255)                                                                                            ggTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCANCAAGCACGACAGTCGGTCCTCCATCAATACATNATAATGCTCGTGTCCGGCCTGCCAGTAGGTCAGCAGCGCGCGCCCTGGCAAGGGGATCACTTCCATATGGAGTGGTAACCGCGCACAGCACGCAAGGGCAAGGGTGATGAAACTAACCAGCGCa                                                                                                                                                                                                             >  2:591562/1‑260 (MQ=255)                                                                                            ggTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCAACGGCGAGGGCTGCACCGACAAACAGTGCCGGCANCAAGCGCGGCAGTCGGTACTCCATCAATACATNATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAAGGCAAGGGTGATGCAAATAACCAGCGCAATTTTCATACTCGACTCCTAACAAGCcaaacaaa                                                                                                                                                                           >  2:591571/1‑294 (MQ=255)                                                                                             gTTAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACTCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAggg                                                                                                                                                                    <  2:638364/300‑1 (MQ=255)                                                                                              ttAACACCGAGAATATCNGTTGATGCCAGAGGGTTGNGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTCCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGNCAGACAAAGCAAGGGc                                                                                                                                                                   <  1:666813/300‑1 (MQ=255)                                                                                               tAACACCGAGAATATCCGTTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCt                                                                                                                                                                  <  2:392432/300‑1 (MQ=255)                                                                                                          atatCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAgc                                                                                                                                                        >  1:434602/1‑299 (MQ=255)                                                                                                                 gTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCNCGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGTTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCAc                                                                                                                                                <  2:558445/300‑1 (MQ=255)                                                                                                                  tGATGCCAGAGGGTTTCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCNCGGGGATCACTTCCATATGGAGTGATAACAGCNCACAGCCCGCCAGGGCAATGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCAc                                                                                                                                                <  2:602207/299‑1 (MQ=255)                                                                                                                     tGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAATAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTAATCCTCGACCCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTgc                                                                                                                                             >  1:417372/1‑299 (MQ=255)                                                                                                                     tGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTgc                                                                                                                                             >  1:442537/1‑299 (MQ=255)                                                                                                                       ccAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGACAATCAGCGCCAGCACTGCGcc                                                                                                                                          >  2:300774/1‑300 (MQ=255)                                                                                                                              gTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGNAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGc                                                                                                                                   >  2:573176/1‑300 (MQ=255)                                                                                                                              gTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCAAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTACGCCTGCGGGc                                                                                                                                   >  2:299050/1‑300 (MQ=255)                                                                                                                                               gTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGNCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCANGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCAGGGGAAGGc                                                                                                                  >  2:583757/1‑300 (MQ=255)                                                                                                                                               gTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCACGCCACAGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAACCCGCCAAGGCAAGGGTGATGAAAATAACCAGCGCAAt                                                                                                                                                                                                           >  2:137542/1‑211 (MQ=255)                                                                                                                                                   cAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTNCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAgc                                                                                                              >  1:170696/1‑300 (MQ=255)                                                                                                                                                    aGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGACAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCa                                                                                                                >  1:622776/1‑297 (MQ=255)                                                                                                                                                     gCACGCCCGCCACGGTGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAACGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAgcgc                                                                                                            <  2:691086/300‑1 (MQ=255)                                                                                                                                                     gCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCCGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAgcgc                                                                                                            <  2:691080/300‑1 (MQ=255)                                                                                                                                                                   gCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTNCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACAt                                                                                              <  1:565874/300‑1 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGt                                                                                                                                                                                                                                >  1:94172/1‑166 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGt                                                                                                                                                                                                                                <  2:94172/166‑1 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGt                                                                                                                                                                                                                                <  2:94009/166‑1 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGc                                                                                          >  2:476705/1‑300 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTACTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGc                                                                                          >  1:235668/1‑300 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCAACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTACGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGc                                                                                          >  1:235659/1‑300 (MQ=255)                                                                                                                                                                       gggCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACCCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGt                                                                                                                                                                                                                                >  1:94009/1‑166 (MQ=255)                                                                                                                                                                         gCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCca                                                                                        <  1:31074/300‑1 (MQ=255)                                                                                                                                                                               ccGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAgcagca                                                                                  >  1:386197/1‑300 (MQ=255)                                                                                                                                                                               ccGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCAAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCagcagc                                                                                   >  1:682925/1‑299 (MQ=255)                                                                                                                                                                                 gACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTACGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCAAGCAGCATc                                                                                >  1:549196/1‑300 (MQ=255)                                                                                                                                                                                         gTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGc                                                                        <  1:590491/300‑1 (MQ=255)                                                                                                                                                                                                  gcaAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCcagcag                                                               <  1:116999/300‑1 (MQ=255)                                                                                                                                                                                                          ggCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTc                                                        <  1:583757/299‑1 (MQ=255)                                                                                                                                                                                                                 ggTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAg                                                <  1:673735/300‑1 (MQ=255)                                                                                                                                                                                                                         aTCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgc                                        >  1:731889/1‑300 (MQ=255)                                                                                                                                                                                                                            aaTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCTTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc                                      <  2:136777/299‑1 (MQ=255)                                                                                                                                                                                                                            aaTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgcgc                                      <  1:588998/299‑1 (MQ=255)                                                                                                                                                                                                                            aaTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCAGATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGAGGAAAATAACTAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGAGCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTgtgc                                      <  2:740411/299‑4 (MQ=255)                                                                                                                                                                                                                             aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCCTATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAACGCAATTTTCATCCTCGCCTACTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCACCCAGCAGCATGCTCACTGACAGTACGTTGTGCt                                     >  1:434693/1‑299 (MQ=255)                                                                                                                                                                                                                             aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCANGGCAAGGGTGATGAAAATAACCAGCGCAATTTNCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGAtc                                                                                                                              >  2:591369/1‑210 (MQ=255)                                                                                                                                                                                                                             aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGAtc                                                                                                                              <  1:591369/210‑1 (MQ=255)                                                                                                                                                                                                                             aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCt                                     >  1:430510/1‑299 (MQ=255)                                                                                                                                                                                                                             aTACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCAGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCt                                     >  1:434683/1‑299 (MQ=255)                                                                                                                                                                                                                               aCATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGa                                   >  1:691472/1‑299 (MQ=255)                                                                                                                                                                                                                               aCATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGCGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGAt                                  >  1:684369/1‑300 (MQ=255)                                                                                                                                                                                                                                                           aGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGTTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGTGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGAtg      <  1:448165/300‑1 (MQ=255)                                                                                                                                                                                                                                                           aGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGAtg      <  1:448274/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGNCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGa                   <  1:148134/286‑1 (MQ=255)                                                                                                                                                                                                                                                            gTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGa                   >  2:148134/1‑286 (MQ=255)                                                                                                                                                                                                                                                               ggTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGc  >  1:399523/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggTCAGCAGCGCGCGCCACGGCACGGGGATCACTTCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCAAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCATGCCCAGAAGCGCGCCAGATGTGGc  >  1:233587/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                          ATGCCGCCCGCAAAGGCCAGCAGCGGCAGCACCATCACGGGCAGTGACGGCATAAGAAGTAGAGCCCCCACAGAGGCCAGGCTGGCGGCATGGTTAACACCGAGAATATCCGGTGATGCCAGAGGGTTGCGCACAATCCCCTGTATCAGCACGCCCGCCACGGCGAGGGCTGCACCGACAAACAGTGCCAGCAGCAAGCGCGGCAGTCGGTACTCCATCAATACATAATAATGCTCGTGTCCGGCCTGCCAGTCGGTCAGCAGCGCGCGCCACGGCACGGGGATCACTCCCATATGGAGTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGC  >  NC_000913/4512056‑4512609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑