Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	590,965	C→T	L738L (CTG→CTA)	nfrB ←	cyclic di‑3',5'‑guanylate‑activated glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	590,965	0	C	T	98.9%	307.6 / NA	92	L738L (CTG→CTA)	nfrB	cyclic di‑3',5'‑guanylate‑activated glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/41);  minor base G (0/1);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCCAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCGTGAGCCACTGATGC  >  NC_000913/590669‑591259                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       gACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCTGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTAAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGt                                                                                                                                                                                                                                                                                                     <  1:215406/300‑1 (MQ=255)     aaaaTGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTgcg                                                                                                                                                                                                                                                                                                 <  1:643730/300‑1 (MQ=255)     aaaaTGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTg                                                                                                                                                                                                                                                                                                   <  2:519453/298‑1 (MQ=255)      aaaTGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTAAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTgcgc                                                                                                                                                                                                                                                                                                <  1:400916/300‑1 (MQ=255)          ggCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCATTCATTTTCGGACTCTAGTTGCGCAAcc                                                                                                                                                                                                                                                                                            >  1:205703/1‑300 (MQ=255)               tAGGCTTCCGCAAGGTAGAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGTGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTc                                                                                                                                                                                                                                                                                       <  2:492258/300‑1 (MQ=255)                 ggCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCTGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAGCCTGTTCtg                                                                                                                                                                                                                                                                                     <  2:392053/300‑1 (MQ=255)                 ggCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCt                                                                                                                                                                                                                                                                                      >  1:3212/1‑299 (MQ=255)                           aGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTCT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAAcc                                                                                                                                                                                                                                                                           <  2:539093/300‑1 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCTGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:227682/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCCGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCATTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:418084/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCANACCATCCGATGACGCGATTAAGGTTGTTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:155455/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCANACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:155546/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:751822/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTCTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  1:772279/1‑300 (MQ=255)                            ggTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGAAGCGATTAAGGTTATTCTCATTCATTTTCGGACTCTAGTTGCGCAAACTGTTATGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  2:313302/1‑300 (MQ=255)                             gTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTAAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCCGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCt                                                                                                                                                                                                                                                                          >  2:759545/1‑299 (MQ=255)                                       agtggAATATTATCCTGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGt                                                                                                                                                                                                                                                                 <  2:195168/295‑1 (MQ=255)                                        gCGGAATATTATCCTGCACCTGCTGGTGTATGTATTCAAATTCGTGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCTAGACGGGGATAAATAACAAAATGGCAATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTACTGTGAGTAAt                                                                                                                                                                                                                                                              <  2:353885/300‑1 (MQ=255)                                        gCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAAt                                                                                                                                                                                                                                                              <  2:353893/300‑1 (MQ=255)                                          ggAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAg                                                                                                                                                                                                                                                            <  1:583438/300‑1 (MQ=255)                                          ggAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTAAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAg                                                                                                                                                                                                                                                            <  1:773747/300‑1 (MQ=255)                                          ggAATATTATCCGACACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAg                                                                                                                                                                                                                                                            <  2:187158/300‑1 (MQ=255)                                           gAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATNAATAACAAAATGGCGATAATCGCTCAGCCCCANCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAgc                                                                                                                                                                                                                                                           >  2:583438/1‑299 (MQ=255)                                             atatTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGTTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAATAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCATTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATCGTGa                                                                                                                                                                                                                                                         >  2:229893/1‑300 (MQ=255)                                              tattatCCGACACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCTAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGAt                                                                                                                                                                                                                                                        <  2:178540/300‑1 (MQ=255)                                                ttatCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTGTTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGAtt                                                                                                                                                                                                                                                       >  1:418663/1‑299 (MQ=255)                                                           ctgctgGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACtt                                                                                                                                                                                                                                           >  1:80906/1‑300 (MQ=255)                                                           ctgctgGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCAAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTAGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACt                                                                                                                                                                                                                                            >  2:112917/1‑299 (MQ=255)                                                               ttgtgtATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCtg                                                                                                                                                                                                                                                                                     <  2:392046/252‑1 (MQ=255)                                                                ggTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCNTTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAAt                                                                                                                                                                                                                                      >  1:153472/1‑300 (MQ=255)                                                                ggTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAAt                                                                                                                                                                                                                                      >  1:622199/1‑300 (MQ=255)                                                                         atTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGa                                                                                                                                                                                                                             >  1:68562/1‑300 (MQ=255)                                                                                   gcgGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGCCAGGa                                                                                                                                                                                                                   >  2:220659/1‑300 (MQ=255)                                                                                     ggATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGAcg                                                                                                                                                                                                                 <  2:727356/300‑1 (MQ=255)                                                                                           cGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATCACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTATG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAGGGTTATTTTATTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATcc                                                                                                                                                                                                           >  1:187063/1‑300 (MQ=255)                                                                                           cGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTGTTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATcc                                                                                                                                                                                                           >  1:187428/1‑300 (MQ=255)                                                                                                  ttCGTCGTTATTTTTCTGTGCCTTCGGCGCTTTATCTAGACGTGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGCAATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGt                                                                                                                                                                                                      <  2:415708/298‑1 (MQ=255)                                                                                                     gtcgtTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCt                                                                                                                                                                                                 >  1:413784/1‑300 (MQ=255)                                                                                                                  tgtgCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGcc                                                                                                                                                                                    <  1:684557/300‑1 (MQ=255)                                                                                                                       cTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTc                                                                                                                                                                                 >  1:773538/1‑298 (MQ=255)                                                                                                                         tCAGCGCTTTATCGAGANGGGGATAAATAACAAAATNGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGAGATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCNGATAACGCCTTCGGTg                                                                                                                                                                             <  1:667392/300‑1 (MQ=255)                                                                                                                                                     aaCAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGTACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAAc                                                                                                                                                 <  2:718012/300‑1 (MQ=255)                                                                                                                                                        aaaaTGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATACGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTg                                                                                                                                               <  1:705595/299‑1 (MQ=255)                                                                                                                                                                          agcccccAGCTCTTCTGNCGCTGGTGCCGATATTGTNCTGCGAGCGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGNGTTCATGGCGCAATaa                                                                                                                              <  1:655846/297‑1 (MQ=255)                                                                                                                                                                            gCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGTGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCCATaacaac                                                                                                                          >  2:142260/1‑300 (MQ=255)                                                                                                                                                                            gCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTACTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATaacaac                                                                                                                          >  2:474965/1‑300 (MQ=255)                                                                                                                                                                            gCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTAATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTACTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCAGAGCGGCAGAGAACTGCGTTCATGGCGCAATaacaac                                                                                                                          >  2:305258/1‑300 (MQ=255)                                                                                                                                                                            gCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTACTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATaacaac                                                                                                                          >  2:533762/1‑300 (MQ=255)                                                                                                                                                                            gCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCATTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATaacaac                                                                                                                          >  1:288740/1‑300 (MQ=255)                                                                                                                                                                             ccccAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAAACTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAacaaca                                                                                                                         >  2:170131/1‑300 (MQ=255)                                                                                                                                                                                  gCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCATTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAGTAACAACACGTTa                                                                                                                    >  2:645419/1‑300 (MQ=255)                                                                                                                                                                                                  tGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTACTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTa                                                                                                     >  2:627999/1‑300 (MQ=255)                                                                                                                                                                                                      gATATTGT‑CTGCGAGTGCGCTGGTACTAAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTANTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTNGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAatat                                                                                                 >  2:588280/1‑300 (MQ=255)                                                                                                                                                                                                      gATATTGT‑CTGCGAGTGCGCTGGTACTAAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAatat                                                                                                 >  2:186070/1‑300 (MQ=255)                                                                                                                                                                                                                tGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATTACGCTATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTTTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCg                                                                                        <  2:437041/300‑1 (MQ=255)                                                                                                                                                                                                                        ggggggTAGTCAATAAAGACGTAAGCAGTAAAACAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTNTGTTTAAACCTGCTTTAAGTAATAGTGATTGCNTCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAAATTGCCGAGTGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGt                                                                                >  2:591673/5‑300 (MQ=255)                                                                                                                                                                                                                        cgcTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTGTTGTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCAGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATCTGACCGAGCGTGGTcggt                                                                                >  2:454393/1‑297 (MQ=255)                                                                                                                                                                                                                        cgcTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGt                                                                                >  2:454344/1‑300 (MQ=255)                                                                                                                                                                                                                        cgcTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGAAGCGATTAAGGTTGTTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTNTGTTTAAACCTGCTTTGAGTAATAGTGATTGCNTCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAACACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACAAGTTAATTGCTGAACGATTAATATGACCGAGCGTGGta                                                                                   >  2:591648/1‑296 (MQ=255)                                                                                                                                                                                                                        cgatGGTACTCAATAAAGACGTCAGCAGTAAACCAGCCCATCCGATGACGCGATTAGGGTTATTCTCATTAATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAGCGCCTTAGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCTTTCATGGCGCAATAACAACACGTTAATTGCTGAAc                                                                                                          >  2:218075/4‑274 (MQ=255)                                                                                                                                                                                                                          cTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTAt                                                                              >  1:50562/1‑300 (MQ=255)                                                                                                                                                                                                                          cTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGCTATTCTCATTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCCGGACGCGATCCAAAGTTTCCTGGCTGATAACGCCTTCGGTGACAAAAAACTTGCCGAGCGGCAGAGAACTGCGTCCATGGCGAAATAACAACACGTTAATTGc                                                                                                               >  2:444088/1‑267 (MQ=255)                                                                                                                                                                                                                             gTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAGGGTTGTTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCAGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTAttt                                                                            >  2:428066/1‑299 (MQ=255)                                                                                                                                                                                                                             gTACTAAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTTTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATGAATATGACCGAGCGTGGTCAGTATTTc                                                                           <  1:588280/300‑1 (MQ=255)                                                                                                                                                                                                                             gTACTAAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCAAGCGTGGTCAGTATTTc                                                                           <  1:186070/300‑1 (MQ=255)                                                                                                                                                                                                                               aCTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCgg                                                                         <  1:712261/300‑1 (MQ=255)                                                                                                                                                                                                                                    aTAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCGTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAAc                                                                    >  1:270982/1‑300 (MQ=255)                                                                                                                                                                                                                                          aCGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAAc                                                              <  2:636665/300‑1 (MQ=255)                                                                                                                                                                                                                                           cGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACt                                                             >  1:446743/1‑300 (MQ=255)                                                                                                                                                                                                                                               agcagTAAACCAGACCATCCGATTACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGGTGAATTGTCAGGACTCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATg                                                         <  2:418084/300‑1 (MQ=255)                                                                                                                                                                                                                                                gcagTAAACCAGACCATCCGATGACGCGATTAAGGTTGTTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCAGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGc                                                        >  2:696242/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cagTAAACCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGc                                                        >  1:455155/1‑299 (MQ=255)                                                                                                                                                                                                                                                 cagTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGc                                                        >  1:455318/1‑299 (MQ=255)                                                                                                                                                                                                                                                 cagTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACAAGTTAATTGCTGAACGATTAATATGAACGAGCGTGGTCAGTATTTCGGCGAACAGGACCTGATGc                                                        >  2:27862/1‑299 (MQ=255)                                                                                                                                                                                                                                                     aaaCCAGACCATCCGATGACGCGATTAAGGTTATTTTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGAACGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGcac                                                   >  2:653631/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGTACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACatat                                               <  2:149532/299‑1 (MQ=255)                                                                                                                                                                                                                                                           gACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTCCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATAtt                                              <  2:207592/299‑1 (MQ=255)                                                                                                                                                                                                                                                            aCCATCCGATGACGCGGTTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATATTGATTGCATCGAAACTTGTAATTCGCTTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCTGCACATAtt                                              <  2:167009/298‑1 (MQ=255)                                                                                                                                                                                                                                                               aTCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGTACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTgccgc                                         <  2:53616/300‑1 (MQ=255)                                                                                                                                                                                                                                                               aTCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAATTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTgccgc                                         <  2:53705/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTATTCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAGTAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAAAACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCAGACAGATTTCAACGGCctgct                      >  2:103312/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ttCTCCTTTATGTTCGGACTATGGTTGAGCAACCTGTTCGGTGTTTAAACCTGCGTTGAGTAAGAGTGATTGCATCGAAACTTGTAATTCGCTTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGAGAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCGGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGTTGTTc                  <  2:227682/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ttCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTTTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTc                  <  2:751822/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ttCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCctgctg                     <  2:356557/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ttCTCCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTc                  <  1:313302/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ttCTCCTTCATTTTCGGACTCGAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATANCGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGTGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTc                  <  2:246019/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         tcCTTCATTTTCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCg                <  2:58061/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ttttCGTACTCTAGTTGCGCAACCTTTTCTGTGTTTAAACCTTCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACACCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCGTGAGCCAc        <  2:155455/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ttttCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCGTGAGCCAc        <  1:759545/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ttgtCGGACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCGTGAGCCAc        <  2:155546/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      gACTCTAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACACATTGCCGACAGATTTCAACGGCATGCTGTTCCGTGAGCCACTGATGc  >  2:38698/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       GACCAAAATGGCGATAGGCTTCCGCAAGGTATAAAGTCAGCGGAATATTATCCGGCACCTGCTGGTGTATATATTCAAATTCGCGGATGGCGGTTGCTTCGTCGTTATTTTTCTGTGCCTTCAGCGCTTTATCGAGACGGGGATAAATAACAAAATGGCGATAATCGCTCAGCCCCAGCTCTTCTG‑CGCTGGTGCCGATATTGT‑CTGCGAGTGCGCTGGTACTCAATAAAGACGTCAGCAGTAAACCAGACCATCCGATGACGCGATTAAGGTTATTCTCCTTCATTTTCGGACTCCAGTTGCGCAACCTGTTCTGTGTTTAAACCTGCTTTGAGTAATAGTGATTGCATCGAAACTTGTAATTCGCGTTGAATTGTCAGGACGCGATCCAACGTTTCCTGGCTGATAACGCCTTCGGTGACCAAAAACTTGCCGAGCGGCAGAGAACTGCGTTCATGGCGCAATAACAACACGTTAATTGCTGAACGATTAATATGACCGAGCGTGGTCAGTATTTCGGCGAACAGGAACTGATGCGGCACATATTGCCGCCAGATTTCACCGGCCTGCTGTTCCGTGAGCCACTGATGC  >  NC_000913/590669‑591259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑