Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,826	G→A	G330D (GGT→GAT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,826	0	G	A	100.0%	194.1 / NA	60	G330D (GGT→GAT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/22);  total (38/22)

GAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTT  >  NC_000913/3787534‑3788105                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                        gAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACTAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGa                                                                                                                                                                                                                                                                                   <  1:109971/299‑1 (MQ=255) gAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGa                                                                                                                                                                                                                                                                                   <  1:107200/299‑1 (MQ=255)  aaTCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAg                                                                                                                                                                                                                                                                                 <  1:297383/300‑1 (MQ=255)        gTCTGCGTAACAGCATTNCCCGTGCGGAAGCCGCGGNGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGANGATACTGAAGTTAAAg                                                                                                                                                                                                                                                                           <  1:651677/300‑1 (MQ=255)         tCTGCGTAACAGCATTGCCCGTGCGGAGGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACTTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGTGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATGCTGAAGTTAAAGc                                                                                                                                                                                                                                                                          <  2:169066/300‑1 (MQ=255)                            cGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGt                                                                                                                                                                                                                                                        >  1:128655/1‑299 (MQ=255)                                 ggAAGCCGCGGCGAAAGNGCGTGCAGAACGAGAAGCNCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCNACGGTCGGGTGATTCt                                                                                                                                                                                                                                                  <  1:666637/300‑1 (MQ=255)                                          ggcgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAAGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTg                                                                                                                                                                                                                                         <  2:189778/300‑1 (MQ=255)                                                          gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGt                                                                                                                                                                                                                         <  1:701318/300‑1 (MQ=255)                                                          gAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACCCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAAGCTACGGt                                                                                                                                                                                                                         >  2:641948/1‑300 (MQ=255)                                                           aaCGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTc                                                                                                                                                                                                                        <  1:273356/300‑1 (MQ=255)                                                            aCGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCt                                                                                                                                                                                                                       <  2:401137/300‑1 (MQ=255)                                                                   gCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggt                                                                                                                                                                                                                 >  1:453365/1‑299 (MQ=255)                                                                       gTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggt                                                                                                                                                                                                              >  2:44980/1‑298 (MQ=255)                                                                           ggCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTTGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGa                                                                                                                                                                                                        >  1:308522/1‑300 (MQ=255)                                                                           ggCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGa                                                                                                                                                                                                        >  1:408976/1‑300 (MQ=255)                                                                                aGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGc                                                                                                                                                                                                      <  1:373910/297‑1 (MQ=255)                                                                                     gTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGACAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTaaa                                                                                                                                                                                              >  1:762172/1‑300 (MQ=255)                                                                                          cgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTATGGTGGTGGTGGTTGAGCATGGTAAAGGCGa                                                                                                                                                                                         >  2:180711/1‑300 (MQ=255)                                                                                                             aCGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCAGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCtat                                                                                                                                                                      >  1:323303/1‑300 (MQ=255)                                                                                                                 gcAAAGGCACCGCCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCATGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGTTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                                                                                  <  2:155708/300‑1 (MQ=255)                                                                                                                 gcAAACGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                                                                                  <  1:776889/300‑1 (MQ=255)                                                                                                                  cAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCa                                                                                                                                                                 >  1:303503/1‑300 (MQ=255)                                                                                                                      ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGc                                                                                                                                                                                                      >  2:394701/1‑259 (MQ=255)                                                                                                                      ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGc                                                                                                                                                                                                      <  1:394701/259‑1 (MQ=255)                                                                                                                             ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCGTCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                                                                        >  2:683666/1‑298 (MQ=255)                                                                                                                             ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                                                                        >  2:638478/1‑298 (MQ=255)                                                                                                                             ccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                                                                        >  1:402492/1‑298 (MQ=255)                                                                                                                               tACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGNCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTg                                                                                                                                                    <  2:605346/300‑1 (MQ=255)                                                                                                                                aCAAACCGACCGAAAGCGAAAAAGCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGTTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGTTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                                                                                  <  2:400386/285‑1 (MQ=255)                                                                                                                                  aaaCCGACCGAAAGCGAAAAATCGTTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCATGCATTCTTGCCGGTTCGCGTGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGTTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGTTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGc                                                                                                                                                 <  2:18812/300‑1 (MQ=255)                                                                                                                                  aaaCCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGc                                                                                                                                                 <  1:582254/300‑1 (MQ=255)                                                                                                                                           gaAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTc                                                                                                                                         <  1:179421/299‑1 (MQ=255)                                                                                                                                                               gTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGcc                                                                                                                    >  1:534189/1‑300 (MQ=255)                                                                                                                                                               gTCCAGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGCAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGCCATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGcc                                                                                                                    >  2:297977/1‑300 (MQ=255)                                                                                                                                                                            ggtCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACtggta                                                                                                       >  2:450568/1‑299 (MQ=255)                                                                                                                                                                            ggtCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACtggt                                                                                                        >  2:450579/1‑299 (MQ=255)                                                                                                                                                                                 gggCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGgcag                                                                                                  <  1:603923/300‑1 (MQ=255)                                                                                                                                                                                          gcgTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACGGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGc                                                                                           >  2:351317/1‑298 (MQ=255)                                                                                                                                                                                                      aTTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGcc                                                                             >  2:268632/1‑300 (MQ=255)                                                                                                                                                                                                      aTTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGcc                                                                             >  1:73059/1‑300 (MQ=255)                                                                                                                                                                                                                gTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTAt                                                                   >  1:195748/1‑300 (MQ=255)                                                                                                                                                                                                                gTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGAAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTAt                                                                   >  1:194487/1‑300 (MQ=255)                                                                                                                                                                                                                gTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTATGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTAt                                                                   >  1:194493/1‑300 (MQ=255)                                                                                                                                                                                                                gTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTAAGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCAATGGTGGGCAGCAGTGGCGGTCAGGGTAGGCCTTCACTCTAt                                                                   >  1:195466/1‑300 (MQ=255)                                                                                                                                                                                                                            aCGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgc                                                       >  2:385871/1‑300 (MQ=255)                                                                                                                                                                                                                               cTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgccgc                                                    >  1:755620/1‑300 (MQ=255)                                                                                                                                                                                                                                 gCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgcc                                                      >  2:383075/1‑296 (MQ=255)                                                                                                                                                                                                                                           ggCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGc                                         >  2:786244/1‑299 (MQ=255)                                                                                                                                                                                                                                              gAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACGGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc                                     >  2:691325/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAAt                                  >  1:79649/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCACTcc                                >  2:463488/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATcc                                >  1:419946/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCAGGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATcc                                >  2:543054/1‑300 (MQ=255)                                                                                                                                                                                                                                                        aGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATNAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTNGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGc                           <  2:585673/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGc                           <  2:453365/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGt                        >  2:17520/1‑298 (MQ=255)                                                                                                                                                                                                                                                             gAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAAGCGACACGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCGGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGACAGGGTCAGGCGGTCACTCcacaacggt                        >  2:17385/1‑293 (MQ=255)                                                                                                                                                                                                                                                                            ggTATGGTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGtt       >  2:343941/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 ggTTATCGGTGCTTCTGAAGATACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCAAGGGTCAGGCGGTCAATCCACAGCAGTGGTTGGGAAGATAAGTTTTGtt  >  2:49071/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                        GAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGGTTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGATAAGTTTTGTT  >  NC_000913/3787534‑3788105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑