Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,444,631	C→T	T527T (ACC→ACT)	ydbH →	putative phospholipid transport factor YdbH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,444,631	0	C	T	100.0%	209.3 / NA	61	T527T (ACC→ACT)	ydbH	putative phospholipid transport factor YdbH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

TGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCGTGTTGAAAGGCGGTAGTGCCTGGA  >  NC_000913/1444340‑1444923                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                     tggccgctggcAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAAt                                                                                                                                                                                                                                                                                               <  1:496761/299‑1 (MQ=255)                           gAAAAGGGAGGTTTACACCGATGAATGCCACCTTGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGACCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGGAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATcctacct                                                                                                                                                                                                                                                                    <  2:17828/300‑1 (MQ=255)                            aaaaGGGAGTTTTACACCGATGAAGGCCACCTTGGATGGCGCAGGAAAAGTTGAGTGGCATGACAGCACGATTGCGCTGACCGATCTCTTCACCGGTTTCGACCAGTTACAATACTATACGATGACCTTAGAAACGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACTTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATcctaccc                                                                                                                                                                                                                                                                    <  2:17850/299‑2 (MQ=255)                                    gTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATc                                                                                                                                                                                                                                                                          >  2:704838/1‑285 (MQ=255)                                    gTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATc                                                                                                                                                                                                                                                                          <  1:704838/285‑1 (MQ=255)                                    gTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTAACTATTTCctct                                                                                                                                                                                                                                                           >  1:65713/1‑300 (MQ=255)                                    gTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTACTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTAACTATTTCctct                                                                                                                                                                                                                                                           >  2:124801/1‑300 (MQ=255)                                      tttACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGATTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGAGCCTACCTATTTCctcgtt                                                                                                                                                                                                                                                         <  1:84380/300‑4 (MQ=255)                                           acCGATGAATGCCACCTGGGATGTCGCAGTAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAgg                                                                                                                                                                                                                                                    <  2:336756/300‑1 (MQ=255)                                              gATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGa                                                                                                                                                                                                                                                 >  1:737727/1‑300 (MQ=255)                                              gATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTATTTAAAGGCGa                                                                                                                                                                                                                                                 >  1:737740/1‑300 (MQ=255)                                                       cACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTTTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGc                                                                                                                                                                                                                                        >  1:776050/1‑300 (MQ=255)                                                        aCCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGc                                                                                                                                                                                                                                        <  1:545708/299‑1 (MQ=255)                                                                          aaaGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGtt                                                                                                                                                                                                                     <  1:223167/300‑1 (MQ=255)                                                                            aGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTc                                                                                                                                                                                                                    >  1:607493/1‑299 (MQ=255)                                                                            aGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTACTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTAACTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTc                                                                                                                                                                                                                    >  1:608001/1‑299 (MQ=255)                                                                                 aGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTa                                                                                                                                                                                                               <  1:717012/299‑1 (MQ=255)                                                                                   tGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTACTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACNTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAAt                                                                                                                                                                                                            >  2:617011/1‑300 (MQ=255)                                                                                                cGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGAc                                                                                                                                                                                                >  1:121580/1‑299 (MQ=255)                                                                                                    tACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTAt                                                                                                                                                                                           <  1:703334/300‑1 (MQ=255)                                                                                                         tGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACANGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTc                                                                                                                                                                                      <  1:564926/300‑1 (MQ=255)                                                                                                         tGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTc                                                                                                                                                                                      <  2:469763/300‑1 (MQ=255)                                                                                                         tGACCGACCTCTCCACCGGTTTCGACCATTTACAATACGGTACGATTACGGTAGAAAAGCCGCGATTAATTCTCGACANGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCTGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTTGGACGGTATTCGTc                                                                                                                                                                                      <  1:157102/300‑1 (MQ=255)                                                                                                                   ctcCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTACTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTCAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTaa                                                                                                                                                                            >  2:416507/1‑300 (MQ=255)                                                                                                                                    aGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAAc                                                                                                                                                           <  2:481164/300‑1 (MQ=255)                                                                                                                                    aGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCACTGTCACTGGACGCCGGGCAAACGCTGTTCACTGTCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTTGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAAc                                                                                                                                                           <  2:187767/300‑1 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGGGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  2:532772/1‑300 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  1:41097/1‑300 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACAGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  2:142659/1‑300 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTACTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  2:217586/1‑300 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCATAATTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGATGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  1:40643/1‑300 (MQ=255)                                                                                                                                     gTTACAATACGGTACGATGAAGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCAGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACa                                                                                                                                                          >  2:91812/1‑300 (MQ=255)                                                                                                                                      ttACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACaa                                                                                                                                                         >  2:273091/1‑300 (MQ=255)                                                                                                                                          aaTACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCTGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCAc                                                                                                                                                     <  2:772689/300‑1 (MQ=255)                                                                                                                                          aaTACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTTGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCAc                                                                                                                                                     <  1:687361/300‑1 (MQ=255)                                                                                                                                          aaTACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCAc                                                                                                                                                     <  1:404364/300‑1 (MQ=255)                                                                                                                                                gTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGAcc                                                                                                                                                >  1:224170/1‑299 (MQ=255)                                                                                                                                                          ggTAGAAAAGCCGCGATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGc                                                                                                                                      <  1:8130/299‑1 (MQ=255)                                                                                                                                                                        gATTAATTCTCGACAAGCTCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCAccc                                                                                                                        <  1:403525/299‑1 (MQ=255)                                                                                                                                                                                    acaagtttaTCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAGCGCTGTTCACTGGCGGCAGTGTGTTACTGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGAt                                                                                                             <  2:677397/290‑1 (MQ=255)                                                                                                                                                                                                         gTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGaa                                                                                          >  1:538949/1‑296 (MQ=255)                                                                                                                                                                                                         gTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGaa                                                                                          >  2:449958/1‑296 (MQ=255)                                                                                                                                                                                                            aCGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTAt                                                                                    >  1:65689/1‑299 (MQ=255)                                                                                                                                                                                                                   gCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGGTCCTACCTATTTCCTATTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCAACCGACTGGAAGACGAACTTACGCGATGGTGAACTGTATGCTCAGGt                                                                            >  2:326444/1‑300 (MQ=255)                                                                                                                                                                                                                    cATCCCTCCTTTAGCGGCGCGCTTTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCTTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCa                                                                               <  2:40643/296‑1 (MQ=255)                                                                                                                                                                                                                     aTCCCTCCTTTAGCGGCGCGCTGTCACTGGACACCGGGCAAACGCTGTTCACGGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGTGCGCAATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTTGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCa                                                                               <  2:41097/295‑1 (MQ=255)                                                                                                                                                                                                                          tcctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCCGGTTGCAttt                                                                     >  1:389874/1‑300 (MQ=255)                                                                                                                                                                                                                          tcctTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCAttt                                                                     >  1:389785/1‑300 (MQ=255)                                                                                                                                                                                                                                 gcggcgCGCTGTCACGGGACGCCGTGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGTTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTctgctg                                                              <  2:639718/300‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCt                                                         >  1:257191/1‑300 (MQ=255)                                                                                                                                                                                                                                      gcgcTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTAACTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCt                                                         >  1:256682/1‑300 (MQ=255)                                                                                                                                                                                                                                         cTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACACGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGaa                                                      >  2:363205/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ggCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCcgcgc                                        >  1:541544/1‑298 (MQ=255)                                                                                                                                                                                                                                                         ggCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTAACTATTTCCTATTTACAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCACTTTCTGCTGCGCCTGAACAAGGATTCcgcgc                                        >  2:270687/1‑298 (MQ=255)                                                                                                                                                                                                                                                                   gTTCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGacac                              >  1:209464/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    ttCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTCGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGc                           <  2:690359/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCGACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTNGGACGGTATTCGTCTGCGCGTTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGacac                              <  2:238008/297‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCACTGGCGGCAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCg                          <  1:124801/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gcggcAGTGTGTTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTATTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCATGAACAAGGATTCCGCGCGGGAGGACACGGCGTGtt                      >  2:353258/1‑298 (MQ=255)                                                                                                                                                                                                                                                                               ccgtgtgtTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCGTGTTGaaa                  >  1:622810/3‑298 (MQ=255)                                                                                                                                                                                                                                                                                 gtgtgtTACCGCCATCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCGTGTTGAAAGGc               >  1:629610/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                              aTCAACTTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCGTGTTGAAAGGCGGTAGTGCCTGGa  <  2:737727/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                     TGGCCGCTGGCAGTGGCGCTACTGGGGAAAAGGGAGTTTTACACCGATGAATGCCACCTGGGATGTCGCAGGAAAAGGTGAGTGGCATGACAGCACGATTACGCTGACCGATCTCTCCACCGGTTTCGACCAGTTACAATACGGTACGATGACGGTAGAAAAGCCGCGATTAATTCTCGACAAGCCCATCGTCTGGGTACGTGACGCACAGCATCCCTCCTTTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGCATTTTCTGCTGCGCCTGAACAAGGATTCCGCGCGGGAGGACACGGCGTGTTGAAAGGCGGTAGTGCCTGGA  >  NC_000913/1444340‑1444923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑