Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,517,591	C→T	D43N (GAT→AAT)	fecR ←	ferric citrate regulator FecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,517,591	0	C	T	100.0%	146.1 / NA	43	D43N (GAT→AAT)	fecR	ferric citrate regulator FecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/27);  total (16/27)

ATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTGCGAAAGCAGAAACGCTTCACGTGTTTTGCCGTTTAGCCCGTCC  >  NC_000913/4517304‑4517882                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    atccttcgcgCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACTTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATAcc                                                                                                                                                                                                                                                                                          <  2:128112/293‑1 (MQ=255)  tCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGTAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCTGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCAc                                                                                                                                                                                                                                                                                        <  1:671681/300‑1 (MQ=255)  tCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCAc                                                                                                                                                                                                                                                                                        <  1:671680/300‑1 (MQ=255)          gcTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGcc                                                                                                                                                                                                                                                                                 <  1:83802/299‑1 (MQ=255)            tttGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAgc                                                                                                                                                                                                                                                                              <  2:693036/300‑1 (MQ=255)               gggTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAgcgc                                                                                                                                                                                                                                                                            >  2:756018/1‑299 (MQ=255)                            gAGCAGGGAGCCATCTTCCATTTGCTGACTGCTTACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACTTGACGGCAGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCACCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTgg                                                                                                                                                                                                                                                              <  2:157610/300‑1 (MQ=255)                            gAGCAGGGAGCCATCTTCCATTTGCTGACGGCTGACTGTGTCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTgg                                                                                                                                                                                                                                                              <  2:152299/300‑1 (MQ=255)                                                       aCGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGc                                                                                                                                                                                                                                   <  1:385233/300‑1 (MQ=255)                                                            tGACTGTGCCTTTTGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATAcc                                                                                                                                                                                                                              <  1:223557/300‑1 (MQ=255)                                                            tGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATAcc                                                                                                                                                                                                                              <  1:223358/300‑1 (MQ=255)                                                             gACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCa                                                                                                                                                                                                                             >  1:725119/1‑300 (MQ=255)                                                              actattccTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACTTGACGGCGGGTGAGGCGGGTATCGTGCAACGCTCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGTTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAACACGGCATACCAg                                                                                                                                                                                                                            <  2:179581/294‑1 (MQ=255)                                                              aCTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAg                                                                                                                                                                                                                            <  1:647007/300‑1 (MQ=255)                                                                   gCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTACTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGa                                                                                                                                                                                                                       >  1:317750/1‑300 (MQ=255)                                                                             gtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGNGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGNCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGc                                                                                                                                                                                                             >  2:602950/1‑300 (MQ=255)                                                                               gcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCAAGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCa                                                                                                                                                                                                            >  1:44118/1‑299 (MQ=255)                                                                                                                        atagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTTATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACAGCGGGAATCGGTTAACAAAGGATTCATAAccc                                                                                                                                                                  <  2:85780/298‑1 (MQ=255)                                                                                                                        agagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGAAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAAcc                                                                                                                                                                   <  1:726812/299‑1 (MQ=255)                                                                                                                         gagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCNGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCa                                                                                                                                                                 >  1:156722/1‑300 (MQ=255)                                                                                                                         gagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAAACCa                                                                                                                                                                 >  2:207837/1‑300 (MQ=255)                                                                                                                         gagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTCAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCa                                                                                                                                                                 >  2:208096/1‑300 (MQ=255)                                                                                                                         gagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGATGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCa                                                                                                                                                                 >  2:787889/1‑300 (MQ=255)                                                                                                                              gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCNGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTc                                                                                                                                                            <  2:572390/300‑1 (MQ=255)                                                                                                                                                 gagtaaCAGCAGTAATCCCTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAgcaggc                                                                                                                                           <  2:323728/294‑1 (MQ=255)                                                                                                                                                  aGCAACAGCAGTAATCCCTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACTGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAgcaggcag                                                                                                                                         <  2:191495/299‑1 (MQ=255)                                                                                                                                                         gcagTAATCCTTTCATCACTGGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCATGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTTTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTTGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGAc                                                                                                                                 <  2:317503/300‑1 (MQ=255)                                                                                                                                                         gcagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGAc                                                                                                                                 <  1:90994/300‑1 (MQ=255)                                                                                                                                                                             gaaccgcggGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAAcagcag                                                                                                                                             <  1:261789/263‑1 (MQ=255)                                                                                                                                                                                    gggTGAAGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAAcagcag                                                                                                                                             <  1:261884/261‑1 (MQ=255)                                                                                                                                                                                             gggTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGAGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGa                                                                                             <  1:439827/300‑1 (MQ=255)                                                                                                                                                                                               gTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGaaa                                                                                           >  1:622454/1‑300 (MQ=255)                                                                                                                                                                                               gTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGAAGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGaaa                                                                                           >  1:622437/1‑300 (MQ=255)                                                                                                                                                                                                    gTGCAACGCCCGGCTGGCAACGTCGCCACGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCATGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTTGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAacac                                                                                        <  2:652981/299‑1 (MQ=255)                                                                                                                                                                                                                       aCGTCGCCA‑GGCACATCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCGACCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACTGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGc                                                                   <  2:396139/300‑1 (MQ=255)                                                                                                                                                                                                                            gCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTAt                                                              >  2:184446/1‑300 (MQ=255)                                                                                                                                                                                                                            gCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAAACTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGAAGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTAt                                                              >  2:85176/1‑300 (MQ=255)                                                                                                                                                                                                                            gCCA‑GGCACACCGCAAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGAAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTAATAAACCATACTCAAGAAGGAACAGCAGGCAGTGCTCGCAGGCTTTCGCAACGTATTTTTTCACGGAGCTGATGGAAAAACCGAGCTTGTGCGCAATCTCACTGTa                                                               >  2:504225/1‑299 (MQ=255)                                                                                                                                                                                                                                    acaccgccgagtgtgtcgcgcAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCTTGATTCTGGTCATACCACTGTTGCCAGCGCGCTTCCTGTTTTTGGCTTACGCGCTCGCCGCTTAGCACGGCATACCAGTTGGAAGCTGAACGCAGCGCCTGACGGCGNGAATCGGTTAACAAAGGATTCATAACCCATACNCCAGATGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCATGTATTTTTTCACGGAGTTGATGGAAACACCGAGTTTGTGCGCGATTTCGCTGTATGTCAGAc                                                       <  2:585314/283‑1 (MQ=255)                                                                                                                                                                                                                                    acacCGCCAAGCTGGTTTCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGAc                                                       <  2:671534/300‑1 (MQ=255)                                                                                                                                                                                                                                       ccGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCATGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTAGACGGCTTTCGCCACGTATTTTTTAACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGCCCAt                                                    >  2:8330/1‑300 (MQ=255)                                                                                                                                                                                                                                              gCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTg                                             <  1:411715/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gcCAGGCCCACTGGTTATCCTGATTCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTGCGAAAGCAGAAACGCTTCACGTGtttt                  >  1:105518/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         atcctgattctgTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTGCGAAAGCAGAAACGCTTCACGTGTTTTGCCGTTTAGCCCGTcc  >  2:778991/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         atcctgattctgTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCAACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTGCGAAAGCAGAAACGCTTAACGTGTTTTGCAGTTTAGCCCGTcc  >  2:432695/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    ATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCA‑GGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGGTTAACAAAGGATTCATAACCCATACTCCAGACGGAACAGCAGGCAGTGCTCGACGGCTTTCGCCACGTATTTTTTCACGGAGCTGATGGAAACACCGAGTTTGTGCGCAATCTCGCTGTATGTCAGACCATCCAGTTGCGAAAGCAGAAACGCTTCACGTGTTTTGCCGTTTAGCCCGTCC  >  NC_000913/4517304‑4517882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑