Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,384,743	C→T	H209H (CAC→CAT)	ycjX →	DUF463 domain‑containing protein YcjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,384,743	0	C	T	100.0%	213.2 / NA	62	H209H (CAC→CAT)	ycjX	DUF463 domain‑containing protein YcjX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/28);  total (34/28)

TTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGAC  >  NC_000913/1384461‑1385036                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      ttCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCTGCGAATGGTTGCTCGACCTGCCGATGCTTGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATGGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGgcg                                                                                                                                                                                                                                                                                      <  1:214377/300‑1 (MQ=255) ttCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGTCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCTGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGgcg                                                                                                                                                                                                                                                                                      <  2:347491/300‑1 (MQ=255)     ctgctACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATTCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTACACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCttt                                                                                                                                                                                                                                                                                  >  1:42038/1‑300 (MQ=255)          aCGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCt                                                                                                                                                                                                                                                                              <  1:85213/299‑1 (MQ=255)               aCTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGNGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGcc                                                                                                                                                                                                                                                                          >  2:241997/1‑298 (MQ=255)                    aaGGATACCTCCACGCTGTATCTGGAAATTGTGAATTATCCCGGCGAAGGGTTGCTCGACCTGCCGATGCTGGCGCACGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGgaga                                                                                                                                                                                                                                                                    <  2:740318/299‑1 (MQ=255)                          aCCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCa                                                                                                                                                                                                                                                             >  2:718586/1‑300 (MQ=255)                           ccTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGATTTGTCTTGACGGGAGATATGGCa                                                                                                                                                                                                                                                             >  2:449926/1‑299 (MQ=255)                                     gTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATTGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCcgc                                                                                                                                                                                                                                                  >  1:679778/1‑300 (MQ=255)                                               aTTGTGGATTATCCCGTCGAATGTTTGCTCGACCTGCCGATGCTGGCGTAGGACTATTTAAGCTGTTCGCGCCAGATGACGGGCTTACTCAATGTTCAGCTCGGAGAATGGTCGTCGAAATGGCGAATGATGAGCGAAGGGCGGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGGAGATATGGCAGGTGCGCCCGCGCTGCAAttc                                                                                                                                                                                                                                        <  2:317026/300‑1 (MQ=255)                                               aTTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAAttc                                                                                                                                                                                                                                        >  2:187247/1‑300 (MQ=255)                                                 tgtgGATTAGCCCGGCGAGTGGTTGCTCGACCTGCTGAGGCTTGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGTCTTACTCAATGTTCAACGCGGAGAATGTTCGGCGAAATGGCAAATGATGAGCGAAGGGCTGGACCCGCGAGCGCCGTCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTACCTCCACCACTGTAAAGAGCAGGGGCTGTATTTTAGGCAGCCTGGGCGAGTTGGCGGGACGGGAGATATGGCAggggaggccgtgttgtaattg                                                                                                                                                                                                                                        <  2:13980/298‑20 (MQ=255)                                                    ggATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGTCGCAGGACTATTTAAGCCGGTCGCACCAGATGACGTGCTTACTCACTGGTCAGCGCGGAGAATGGTCGTCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGACTGTCGGACATTGCCGCCGCGTGGACCGATGATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGTTAGATATGGCAGGTGCGCCCGCGCTGCAAttc                                                                                                                                                                                                                                        <  2:317341/295‑1 (MQ=255)                                                                        tGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCt                                                                                                                                                                                                               >  1:221539/1‑300 (MQ=255)                                                                        tGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTACACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATAcc                                                                                                                                                                                                                >  2:393730/1‑299 (MQ=255)                                                                        tGCTCGAACTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCt                                                                                                                                                                                                               >  1:221490/1‑300 (MQ=255)                                                                                                 ggACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGc                                                                                                                                                                                      <  1:599278/300‑1 (MQ=255)                                                                                                 ggACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTACACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCCCACTGGCGCAGGc                                                                                                                                                                                      >  2:382826/1‑300 (MQ=255)                                                                                                      aTTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATa                                                                                                                                                                                 >  1:452809/1‑300 (MQ=255)                                                                                                      aTTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCAGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGTGCAGGCCGATa                                                                                                                                                                                 >  2:660003/1‑300 (MQ=255)                                                                                                      aTTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTACACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATa                                                                                                                                                                                 >  2:271488/1‑300 (MQ=255)                                                                                                       tttAAGCTGTTCGCGCCAGATGGCGGGCTTACTCAGTGGTCAGCGCGGAGAATGGTCGGCGAAATGGCAAATGATGATCGAAGGGCTGGACCCGCTAGCGCCTGCCTACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGTGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATaa                                                                                                                                                                                <  2:117875/300‑1 (MQ=255)                                                                                                               gttCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACtctgtctcttatacacatctcgc                                                                                                                                                                         <  1:587418/297‑23 (MQ=255)                                                                                                                        aGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGaa                                                                                                                                                               <  1:679457/300‑1 (MQ=255)                                                                                                                           tGACGGGCTTACTCAATGTTCAGCGCGGAGAAGGGTCGTCGAAATGGCGAATGAGGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCTCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGTAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTTGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAAtgc                                                                                                                                                            <  2:117176/300‑1 (MQ=255)                                                                                                                              cGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTACAAACTGGCGCAGGCCGATAAGCATACCAATGCAGGAAtgctgc                                                                                                                                                         >  1:8619/1‑300 (MQ=255)                                                                                                                                ggCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTgcgc                                                                                                                                                       >  2:692508/1‑300 (MQ=255)                                                                                                                                     aCTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAgc                                                                                                                                                   >  1:456732/1‑299 (MQ=255)                                                                                                                                     aCTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAAATGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAgc                                                                                                                                                   >  2:638367/1‑299 (MQ=255)                                                                                                                                          aTGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCNTCCCGTGGCAGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTa                                                                                                                                             >  2:621572/1‑300 (MQ=255)                                                                                                                                              tCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGTGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAatta                                                                                                                                         <  1:718586/300‑1 (MQ=255)                                                                                                                                                 gcgcGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAAttatta                                                                                                                                      >  1:113773/1‑300 (MQ=255)                                                                                                                                                            ggTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCCCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAgg                                                                                                                           <  1:110882/300‑1 (MQ=255)                                                                                                                                                                   gAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAAATGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGaa                                                                                                                    <  1:638367/300‑1 (MQ=255)                                                                                                                                                                    aaaTGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAg                                                                                                                   <  1:510115/300‑1 (MQ=255)                                                                                                                                                                    aaaTGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGGGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAg                                                                                                                   <  1:509855/300‑1 (MQ=255)                                                                                                                                                                    aaaTGGCGAATGATGAGCGAAGGGCGGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAg                                                                                                                   <  1:510107/300‑1 (MQ=255)                                                                                                                                                                                    gCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATc                                                                                                   >  1:734837/1‑300 (MQ=255)                                                                                                                                                                                              ggACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGNCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTgc                                                                                          >  2:257952/1‑299 (MQ=255)                                                                                                                                                                                                   cGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTTGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCttt                                                                                     >  1:306294/1‑299 (MQ=255)                                                                                                                                                                                                   cGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGTAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCttt                                                                                     >  1:42964/1‑299 (MQ=255)                                                                                                                                                                                                                  acgaAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCAGTTATCTCCACCACTGTAAAGAGCATGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGTAGGTATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCACAGGCCGATAAGCATACCAATGCCGGAATGCTGCGTGAGCGCTTTAATTATTACTGCTAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGc                                                                     <  2:749178/300‑1 (MQ=255)                                                                                                                                                                                                                                       ttGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACctc                                                >  1:195727/1‑300 (MQ=255)                                                                                                                                                                                                                                       ttGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCAGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACctc                                                >  1:191921/1‑300 (MQ=255)                                                                                                                                                                                                                                              cgcgTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCAGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTAAACa                                          >  2:707837/1‑299 (MQ=255)                                                                                                                                                                                                                                               gcgTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGTAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGt                                        <  1:603736/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCTAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAg                                         <  1:66607/297‑1 (MQ=255)                                                                                                                                                                                                                                                  tGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTTGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAatta                                                                                                                                         <  2:410887/200‑1 (MQ=255)                                                                                                                                                                                                                                                        gATTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGTAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAg                               <  2:452809/300‑1 (MQ=255)                                                                                                                                                                                                                                                         aTTATCTCCACCACTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAgg                              <  1:411836/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     acTGTAAAGAGCATGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGa                    <  1:392642/298‑1 (MQ=255)                                                                                                                                                                                                                                                                     acTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGTGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGNCGATACCTGGGGCGAGTCCAAACTGGCGCAGGNCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGCAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGTGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCAACAGGCATTTAATGata                  >  2:592622/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     acTGTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGata                  >  2:785677/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTNTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGc               >  1:579095/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTAAACAGTGGGCCACAGGCATTTAATGATATGc               >  1:589619/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTACCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGc               >  2:639591/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCAGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTAAACAGTGGGCCACAGGCATTTAATGATATGc               >  1:586196/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAAAGAGCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCAGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCAGATAAGTATAc                                                                                                                                                                          >  2:639643/1‑145 (MQ=255)                                                                                                                                                                                                                                                                             gagCAGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCt           >  1:597668/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                 atgggCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCAc      <  1:430018/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aGGGGCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCAc      <  1:241997/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gCTGCATTTTATTCAGCCTGGGCGCTTTGTCTTGCCTGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTTGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGAc  <  2:490321/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      TTCGCTGCTACGCCACTTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTCGACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTCAATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCGCTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTTAATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGACCGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAATGATATGCGTCTGGCACTGAC  >  NC_000913/1384461‑1385036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑