Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	472,100	C→T	R489C (CGC→TGC)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	472,100	0	C	T	100.0%	184.5 / NA	54	R489C (CGC→TGC)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/26);  total (28/26)

AACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAGCTGGCAG  >  NC_000913/471814‑472382                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                           aaCGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCga                                                                                                                                                                                                                                                                               >  1:420421/1‑300 (MQ=255)    tgAAGGTGAGATTCGCCTTGATGTTCGTCCATGAAGTTCGCTAAGTCACATCGCGCTTCGCCAGGTCGTGGCAATGGTGCAGCAAGATCCGTTGGTGCTGGCGGATACCTTCCTCGCCAACTTGACGCTGGGGCGGGATATCCCCGAAGAACGCGTCTGGCATGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGATGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGAc                                                                                                                                                                                                                                                                            <  2:310257/299‑1 (MQ=255)    ggAAGTTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGTCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACTTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGGGCAACTGGCGGAGCTGGCGCGTAGCATGATCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGAc                                                                                                                                                                                                                                                                            <  1:293660/300‑1 (MQ=255)               ttCGCCTTGATGGTCGTCCATTAAGTTCGCGAAGTCACAGCGCGCTGCGCCAGGGTGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCCCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATcc                                                                                                                                                                                                                                                                 <  2:731715/300‑1 (MQ=255)                  gCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGACGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCAGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTTTCCCTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCt                                                                                                                                                                                                                                                                >  2:292279/1‑298 (MQ=255)                  gCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTACGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGAAGCCGCAAATCCTGa                                                                                                                                                                                                                                                              >  2:292231/1‑300 (MQ=255)                               tcCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGc                                                                                                                                                                                                                                                 >  2:757462/1‑300 (MQ=255)                                 cATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCaa                                                                                                                                                                                                                                               >  1:627156/1‑300 (MQ=255)                                  aTTAAGTTCGCTAATTCACAGCGCGCTGCGCCAGGTCGTGGCAATGGTGCAGCAAGATCCGTTGGTGCTGTCGGATACCTCCCTCACCAACGTGACGCTGGGGCGGGATACCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGTCGGAGCTGGCGCGGAGCATGATCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGAc                                                                                                                                                                                                                                                                            <  2:299299/270‑1 (MQ=255)                                          cGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTACGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCa                                                                                                                                                                                                                                      >  2:512882/1‑300 (MQ=255)                                            ctcaGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCAtt                                                                                                                                                                                                                                    >  1:594482/4‑300 (MQ=255)                                            cTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCAtt                                                                                                                                                                                                                                    >  1:594528/1‑300 (MQ=255)                                              aaGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAANGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGa                                                                                                                                                                                                                                  >  1:146648/1‑300 (MQ=255)                                                        gcgttGCGCCAGGGCTTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACTTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTAc                                                                                                                                                                                                                         <  2:29512/295‑1 (MQ=255)                                                             gcgacAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTAACGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGACATCTCCGAAGAACGCGTATGGCAGATGCTGGAAACCGTGCAACTGGCGGGGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAATTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGCGACGCCGCAATTCCt                                                                                                                                                                                                                                                                >  2:505074/5‑255 (MQ=255)                                                                  aGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACTTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGc                                                                                                                                                                                                                <  1:508433/298‑1 (MQ=255)                                                                     gCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTACGAAGAACGCGTCTGGCAGGCGCGGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGCACAGGCGATTc                                                                                                                                                                                                           >  2:689340/1‑300 (MQ=255)                                                                                gTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGctct                                                                                                                                                                                                 >  1:709876/1‑299 (MQ=255)                                                                                       aaaaTCCGGTGGGGCTGGCGGATACCTTCCTCACCAACTTGACGCTGGGTCGGGATACCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATcc                                                                                                                                                                                                                                                                 <  2:772493/225‑1 (MQ=255)                                                                                              tgtggtgCTGGCGGAGACCTCCCTCACCAACGTGACTCTGGTGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGTCGGAGTTGGCGCGTAGCATGAGCGACGGGATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTg                                                                                                                                                                                   <  2:316732/298‑1 (MQ=255)                                                                                              gttggtattGGCGGAGACCTTTCTCGCCAACGTGACGCTGGGGTGGGATATCTCCGAAGAACGCGTCTGGCAGGCGTTGGAAACCGGGCAACTGGCGGAGCTGGCGTGGAGAATGATCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAACCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCTAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGGACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTg                                                                                                                                                                                   <  2:316772/291‑1 (MQ=255)                                                                                                  gtgCTGGCGGATACCTTCCTCTCCAACTTGACGCTGGGGCGGGAGATCTCCGAAGAACGCGTCGGGCAGGCGCTGGAAACCGTGCAACGGGCGGAGCTGGCGCGGAGCATGAGCGACGGTATTTACACGCCGCTGGTCGAGCAGGGGAATAATCTCTCAGTTGTGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATTCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACAt                                                                                                                                                                              <  1:646519/300‑1 (MQ=255)                                                                                                  gtgCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGTCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGGAGCATGAGTGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACAt                                                                                                                                                                              <  1:646547/300‑1 (MQ=255)                                                                                                  gtgCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACAt                                                                                                                                                                              >  1:465672/1‑300 (MQ=255)                                                                                                           gATACCTTCCTCGCCAACGTGACGCTGGTGCGGGATATCTCCGAAAAACGTGTCTGGCATGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGATGCGTGAACATACCACGCTg                                                                                                                                                                     <  2:230769/300‑1 (MQ=255)                                                                                                                                           ggATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCa                                                                                                                                         >  2:761686/1‑296 (MQ=255)                                                                                                                                                tctcCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATg                                                                                                                                <  1:493635/300‑1 (MQ=255)                                                                                                                                                tctcCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCAGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTACGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCACTGTTGAt                                                                                                                                 >  2:374943/1‑299 (MQ=255)                                                                                                                                                tctacGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGAt                                                                                                                                 >  2:374476/5‑299 (MQ=255)                                                                                                                                                     gaagaccatgTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGANCTGGCACGTAGCATGATCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCATTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAAc                                                                                                                                                                                                                    <  2:241631/202‑1 (MQ=255)                                                                                                                                                             cgTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCt                                                                                                                   >  1:551183/1‑300 (MQ=255)                                                                                                                                                               tCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTgg                                                                                                                 <  1:760926/300‑1 (MQ=255)                                                                                                                                                                  ggCAGGCGCGGGAAACCGTGCAACTGGCGGAGCTGGCGCGGAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTg                                                                                                               <  2:78027/299‑1 (MQ=255)                                                                                                                                                                      ggCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTc                                                                                                           <  1:18723/299‑1 (MQ=255)                                                                                                                                                                         gcTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATc                                                                                                        >  1:122809/1‑299 (MQ=255)                                                                                                                                                                         gcTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCNGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATc                                                                                                        >  2:609005/1‑299 (MQ=255)                                                                                                                                                                         gcTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTACGGTACTGAACAGGCGATTCAACATGCTCNGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGAACATTGTTGATGCCGACACCATTCTGGTGCTTCATc                                                                                                        >  2:608595/1‑299 (MQ=255)                                                                                                                                                                                 ccGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCaa                                                                                                >  1:619854/1‑299 (MQ=255)                                                                                                                                                                                          tgtcgaaactgtcacgTAGCATGAGCGAAGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATTAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAg                                                                                       <  2:739864/285‑1 (MQ=255)                                                                                                                                                                                            gCGGAGCTGGCGCGGAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAg                                                                                    <  1:138392/300‑1 (MQ=255)                                                                                                                                                                                             tggAGCTGGCGCGGAGAATGAGCGACGGGATTTACACGCCGCTGGGCGAGCAGTGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTTATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCTACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGc                                                                                      <  2:78552/296‑1 (MQ=255)                                                                                                                                                                                               gAGCTGGCGCGTAGCATGAGCGACGGTATTTCCACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCCTTCTGGTGTTTCATCGTGGGCACGCCGTGGAGCAGGGc                                                                                 >  2:78083/1‑300 (MQ=255)                                                                                                                                                                                               gAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGc                                                                                 >  2:76562/1‑300 (MQ=255)                                                                                                                                                                                                           aGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGAACATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCaa                                                                     >  2:733051/1‑300 (MQ=255)                                                                                                                                                                                                           aGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGAAAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGAACATTGTTGATGCCGACACCAGTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCAcc                                                                          >  2:732906/1‑295 (MQ=255)                                                                                                                                                                                                                      gggggttcccacGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                                                                                                                 <  2:701599/188‑1 (MQ=255)                                                                                                                                                                                                                       ggggtttACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                                                                                                                 <  2:701467/192‑1 (MQ=255)                                                                                                                                                                                                                              acacGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCTAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCAATCACCAGCAACTGCTGGCGGCCCAGGGAc                                                  <  1:608595/300‑1 (MQ=255)                                                                                                                                                                                                                              acacGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCAATCACCAGCAACTGCTGGCGGCCCAGGGAc                                                  <  1:609005/300‑1 (MQ=255)                                                                                                                                                                                                                                   ccgcTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCACAGGGACGCTAc                                             >  2:501540/1‑300 (MQ=255)                                                                                                                                                                                                                                             aGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCTAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGt                                   <  1:434163/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ggAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAAc                             >  1:621945/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gggCAAAAGCAACTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGaa            >  1:298222/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aaCTGCTGGCACTGGCGTGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAGCTGGcag  <  1:545319/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                           AACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAGCTGGCAG  >  NC_000913/471814‑472382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑