Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,895	C→T	A2T (GCC→ACC)	ybfE ←	ribbon‑helix‑helix domain‑containing protein YbfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,895	0	C	T	100.0%	206.0 / NA	60	A2T (GCC→ACC)	ybfE	ribbon‑helix‑helix domain‑containing protein YbfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/30);  total (30/30)

AATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACATACGGAG  >  NC_000913/711602‑712186                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    aaTTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCATACGGTCCGTTTGTTCTTTGGTCATGAt                                                                                                                                                                                                                                                                                               >  2:121809/1‑300 (MQ=255)               ccTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTc                                                                                                                                                                                                                                                                                   >  1:266757/1‑298 (MQ=255)                  gACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCggggg                                                                                                                                                                                                                                                                              <  2:659867/300‑1 (MQ=255)                                gccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGTTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGcc                                                                                                                                                                                                                                                                  <  1:293687/298‑1 (MQ=255)                                gccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGcc                                                                                                                                                                                                                                                                  <  1:300347/298‑1 (MQ=255)                                   aGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGcc                                                                                                                                                                                                                                                             <  1:725482/300‑1 (MQ=255)                                     ttGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCctc                                                                                                                                                                                                                                                           >  1:748128/1‑300 (MQ=255)                                     ttGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAACGCCTAGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCct                                                                                                                                                                                                                                                            >  1:748122/1‑299 (MQ=255)                                            aTTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGccc                                                                                                                                                                                                                                                      >  1:68723/1‑298 (MQ=255)                                                  aTCTCTTCGATCAGTTCACTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCTTTCAGCTTCAGTTCGACACACTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGTTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCggatggat                                                                                                                                                                                                                                              <  2:553252/300‑1 (MQ=255)                                                       ttCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAATGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAgcgc                                                                                                                                                                                                                                         <  2:463146/300‑1 (MQ=255)                                                               gTTCGCTACTGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCataat                                                                                                                                                                                                                                 <  2:365482/300‑1 (MQ=255)                                                                 tCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATAc                                                                                                                                                                                                                               <  1:719457/300‑1 (MQ=255)                                                                   gCTACTGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCNGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACAt                                                                                                                                                                                                                             <  2:256697/300‑1 (MQ=255)                                                                     tactgCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCTACAGCTTCCGCGTTCAGCTTCAGTTCTACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGTGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATc                                                                                                                                                                                                                            <  2:142562/295‑1 (MQ=255)                                                                     tACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTGCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACAt                                                                                                                                                                                                                             <  2:543425/298‑1 (MQ=255)                                                                        ggCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAgc                                                                                                                                                                                                                         >  1:372700/1‑299 (MQ=255)                                                                                       gactcccccgCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAATGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGcc                                                                                                                                                                                                          <  2:346544/290‑1 (MQ=255)                                                                                                    gTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTNATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTAACGCCAACGCCCAcgcgc                                                                                                                                                                                             >  2:248253/1‑299 (MQ=255)                                                                                                            ccTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGa                                                                                                                                                                                    >  2:719515/1‑300 (MQ=255)                                                                                                                     cTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGc                                                                                                                                                                           >  2:455894/1‑300 (MQ=255)                                                                                                                                 gCTTCAGTTCGACACGNTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCNACGCGCGGAAAGCGACGCNGACGCTGGGTTTTTAAt                                                                                                                                                               >  1:652977/1‑300 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGTTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATc                                                                                                                                                 <  1:178093/299‑1 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACNTCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCa                                                                                                                                                >  1:172138/1‑300 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGAGGCTGGGTTTTTAGTCAGTTGCGTTAATCa                                                                                                                                                >  2:656112/1‑300 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCAAGCCAACGCACACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCa                                                                                                                                                >  2:52531/1‑300 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGAGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCa                                                                                                                                                >  2:475498/1‑300 (MQ=255)                                                                                                                                                gCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCAGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCa                                                                                                                                                >  2:385751/1‑300 (MQ=255)                                                                                                                                                       gCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGAt                                                                                                                                         >  2:750531/1‑300 (MQ=255)                                                                                                                                                       gCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGAt                                                                                                                                         >  1:497726/1‑300 (MQ=255)                                                                                                                                                                         ttttAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTGGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTa                                                                                                                       >  2:687923/1‑300 (MQ=255)                                                                                                                                                                         ttttAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTa                                                                                                                       >  1:681977/1‑300 (MQ=255)                                                                                                                                                                             aGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATAc                                                                                                                   <  1:373803/300‑1 (MQ=255)                                                                                                                                                                                     tcGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGTTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGc                                                                                                                                                                           <  1:743075/235‑1 (MQ=255)                                                                                                                                                                                     gCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGc                                                                                                                                                                           <  1:743987/236‑1 (MQ=255)                                                                                                                                                                                     gCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGc                                                                                                                                                                           >  2:743987/1‑236 (MQ=255)                                                                                                                                                                                     gCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTAGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGc                                                                                                                                                                           >  2:743075/1‑236 (MQ=255)                                                                                                                                                                                              aaTACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGTTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAg                                                                                                  <  2:115442/300‑1 (MQ=255)                                                                                                                                                                                                      tcTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGAcc                                                                                          >  1:699009/2‑300 (MQ=255)                                                                                                                                                                                                          ttCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGt                                                                                      <  2:703639/300‑1 (MQ=255)                                                                                                                                                                                                           tCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATTTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTg                                                                                     <  1:131355/300‑1 (MQ=255)                                                                                                                                                                                                           tCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTg                                                                                     <  1:110938/300‑1 (MQ=255)                                                                                                                                                                                                                             ttAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATcac                                                                   <  1:2077/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggTCGTCTCGTTCGACGCTCGTGCGCGAACAGATCTAATGTCGTATGGTCCGTTTGTTCTTTGGTCATGGTCTTGGTGACGTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGcc                                                            <  2:434561/298‑1 (MQ=255)                                                                                                                                                                                                                                          gtcCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGcc                                                      >  2:421043/1‑300 (MQ=255)                                                                                                                                                                                                                                          gtcCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTAGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTAAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGcc                                                      >  2:699347/1‑300 (MQ=255)                                                                                                                                                                                                                                               gcgagatgtgtataagagacaggATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGCCCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGc                                                       >  1:16903/23‑294 (MQ=255)                                                                                                                                                                                                                                                       cTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGa                                          >  1:619653/1‑299 (MQ=255)                                                                                                                                                                                                                                                                 aaCAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAatc                               <  1:473524/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCGGGCCTCTGCCCGGATGGACAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCAAGTAAAtcatca                           >  2:310515/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gATCTAATGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCTGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAAtcatca                           >  2:85772/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gATCTAATGTCGTACGGTCCGTCTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCAAGTAAatcatc                            >  2:6540/1‑299 (MQ=255)                                                                                                                                                                                                                                                                           atggcttACGGTTCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCGGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  2:140849/294‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGTGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  2:426281/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCATGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTTAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  2:214704/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTTCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  2:549905/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  2:266757/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTGTCGTACGGTCCGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTAc                     <  1:316738/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ttccatttGTTCTTTGTNCATGATCTTGTTGACTTCNGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCNCGGTACTGCTCGCTaa           <  1:652180/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ccGTTTGTTCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCANTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACa         >  1:575268/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              ttCTTTGGTCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACATACGgag  <  1:499446/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    AATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACGCGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGACCCAGTGACCCGCGCCTGCAATCACATGCGCCCGTGCCTGTGGAAATTGAGCCAGTAAATCATCACGGTACTGCTCGCTAACATACGGAG  >  NC_000913/711602‑712186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑