Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,424,524	G→A	noncoding (2260/2904 nt)	rrlD ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,424,524	0	G	A	98.3%	112.5 / NA	60	noncoding (2260/2904 nt)	rrlD	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/35);  total (24/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGCCCTTCCATTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGT  >  NC_000913/3424364‑3424818                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       gATGGCCCTTCCATTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                                                                                                                                             <  1:41467/300‑1 (MQ=14)         ttCCATTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCATTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGTAGAAGACCGCCCCATTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAAGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATcc                                                                                                                                                       <  2:353846/298‑1 (MQ=9)          tCCATTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCAAGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCATCCCACCTATCCTac                                                                                                                                                    >  2:664314/1‑300 (MQ=14)             gttCAGAACCACCGGATCACTATGGCCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTTAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATGAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  2:430631/298‑1 (MQ=9)             aTTCAGAGCCACCGGATCACTATTACCTGCGTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCGGGCTTATGCCAGTGCACTAACCTCCTTATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCNAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  2:570584/299‑1 (MQ=255)             aTTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCTCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  2:212192/299‑1 (MQ=14)             aTTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCTTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  2:78424/299‑1 (MQ=14)             aTTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  2:713782/299‑1 (MQ=14)             aTTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                                  <  1:619088/299‑1 (MQ=14)             aTTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTAcaca                                                                                                                                                 >  1:3371/1‑300 (MQ=14)                      caccGGATCACTATGANCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACANTTCTAAGCCTCCCACCTANCCTACACATCAAGGCTc                                                                                                                                        >  1:658283/1‑300 (MQ=14)                      caccGGATCACTATGANCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGCCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACANTTCTAAGCCTCCCACCTANCCTACACATccacgctc                                                                                                                                        >  1:657821/1‑293 (MQ=9)                               aCTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTTTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                                                >  1:452585/1‑299 (MQ=14)                               aCTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                                                >  1:8293/1‑299 (MQ=14)                                  aTGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCTCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                                            <  2:120851/300‑1 (MQ=14)                                    gACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCa                                                                                                                          >  1:498207/1‑300 (MQ=14)                                        tGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGc                                                                                                                       <  1:648201/299‑1 (MQ=14)                                                 aCCTGCTCGCGCCGTCACGCTCGCATTCAATCTTGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGTAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTaa                                                                                                               <  2:270792/298‑1 (MQ=9)                                                    tGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCATTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCGATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGt                                                                                                           <  2:543476/299‑1 (MQ=14)                                                    tGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGt                                                                                                           <  2:543486/299‑1 (MQ=14)                                                         gcgcCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGTGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACGTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACg                                                                                                      <  2:26475/299‑1 (MQ=14)                                                            ccGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGANGACCGCCCCAGTCAAACTACCCACCAGACACTNTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGt                                                                                                  >  2:599716/1‑300 (MQ=14)                                                                   gCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCg                                                                                           <  2:303781/300‑1 (MQ=14)                                                                       gCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTATGAGAAGACCGCCCCAGTCAAACGACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGc                                                                                                                       <  2:434658/268‑1 (MQ=14)                                                                        cAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTCGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACTCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCtt                                                                                       >  1:386996/1‑299 (MQ=14)                                                                        cAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCAAGGGGTCTTTCCGTCtt                                                                                       >  1:387099/1‑299 (MQ=14)                                                                             aaGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgc                                                                                  >  1:326549/1‑299 (MQ=14)                                                                                  ggCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTac                                                                            >  1:316012/1‑300 (MQ=14)                                                                                     ttATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGTAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTacac                                                                          <  2:661609/299‑1 (MQ=14)                                                                                          ccATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGCGAAGACCGCACCAGTCAAACTAACCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTACCACCTATCCTACACATCGAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACCCTGCATc                                                                    >  1:89407/1‑300 (MQ=255)                                                                                          ccATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                                                    >  1:89263/1‑300 (MQ=14)                                                                                             ttGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTc                                                                 >  1:780293/1‑300 (MQ=14)                                                                                                   tAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGc                                                            <  1:528180/299‑1 (MQ=14)                                                                                                   tAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCg                                                           <  1:518259/300‑1 (MQ=14)                                                                                                      cctcctGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGt                                                        >  2:518693/1‑300 (MQ=14)                                                                                                       ctcctGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTACCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGtt                                                       >  2:127630/1‑300 (MQ=14)                                                                                                             aTGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAttt                                                 <  1:521198/300‑1 (MQ=14)                                                                                                              tgtacGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCACAAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                                                >  2:76326/5‑300 (MQ=9)                                                                                                              tGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                                                <  1:487454/300‑1 (MQ=14)                                                                                                                   gACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCAAGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTg                                            <  1:639204/299‑1 (MQ=14)                                                                                                                       aGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtct                                       <  1:61238/300‑1 (MQ=14)                                                                                                                        ggATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTTCATCTTCACAGCGAGTTCAATTTCACTGAGtctc                                      >  1:497328/1‑300 (MQ=14)                                                                                                                        ggATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtctc                                      >  1:235748/1‑300 (MQ=14)                                                                                                                        ggATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCAAAGCGAGTTCAATTTCACTGAGtctc                                      >  1:689252/1‑300 (MQ=14)                                                                                                                        ggATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCAAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACNTCTAAGCCTACCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACt                                             >  2:237639/1‑293 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCCGTTACTCTTTAGTAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCTGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAc                            <  2:315984/300‑1 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCNTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAc                            <  2:579363/300‑1 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGTAGAc                            <  2:166755/300‑1 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGTAGAc                            <  1:394481/300‑1 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAc                            <  1:394269/300‑1 (MQ=14)                                                                                                                                  aCCTTCGTGCTCCTCAGTTACTCTTTAGTAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTAAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAc                            <  1:593942/300‑1 (MQ=9)                                                                                                                                   ccTTCGTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTNACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACa                           >  1:171379/1‑300 (MQ=14)                                                                                                                                        gTGCTCCTCCGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCt                       >  1:310896/1‑299 (MQ=14)                                                                                                                                            tcctctGTTACTCTTTGTGAGAAGGCCGCCTCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATGACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTGTCCTACACATCAATGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGTTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGcc                   <  2:159223/299‑1 (MQ=11)                                                                                                                                            tcctccGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGcc                   <  1:536035/299‑1 (MQ=14)                                                                                                                                            tcctccGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCca                  <  1:509610/300‑1 (MQ=14)                                                                                                                                            tcctccGTTACTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCGATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGcc                   <  1:537853/299‑1 (MQ=14)                                                                                                                                                     atcatttAGGAGAAGACCGCCCCAGTCAAACTACCCGCCAGACACTGTCCGCAACCTGTATTACGGTTCAACGTGAAAACATCAAACATTAAAGGTTGGTATTTCAAGGTCGGCGCCATGCAGACTAGCGTCCACACTTCTAAGCCTCTCACCTACCCTACACATCGATGCTCAATGTTCAGTGCCAAGCTATAGTAAAAATTCACGGGGTCTGTCCGTCTTGCCGCGGGTACACGGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTACGc         <  2:388882/296‑1 (MQ=11)                                                                                                                                                     aCTCTTTATTAGGAGACCGCCCCAGGCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAAGTCGGCTCCATGCAGACTGGCGTTCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGACTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTACGc         <  2:386742/300‑1 (MQ=9)                                                                                                                                                     aCTCTTTAGGAGAAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGANCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTAAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATATTCACAGCGAGTTCAATTTCACTGAGTCTAGGGTGGAGACAGCCTGGCCATCATTACGc         <  1:561449/300‑1 (MQ=9)                                                                                                                                                             ggagaaGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCANTGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTATTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCGTCATTAAGACATTCGt  >  2:245465/1‑299 (MQ=9)                                                                                                                                                             ggagaaGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCAATCATTACGCCATTCGt  >  2:327571/1‑299 (MQ=14)                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       GATGGCCCTTCCATTCAGAACCACCGGATCACTATGACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTATGCCATTGCACTAACCTCCTGATGTCCGACCAGGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGT  >  NC_000913/3424364‑3424818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑